##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630187.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1323564
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.689322163491905	31.0	31.0	34.0	30.0	34.0
2	31.90805129181513	33.0	31.0	34.0	30.0	34.0
3	32.03903777981269	33.0	31.0	34.0	30.0	34.0
4	35.70627109833752	37.0	35.0	37.0	33.0	37.0
5	35.62827638104391	37.0	35.0	37.0	33.0	37.0
6	35.67552003529863	37.0	35.0	37.0	33.0	37.0
7	35.65433934437625	37.0	35.0	37.0	33.0	37.0
8	35.666326675551765	37.0	35.0	37.0	33.0	37.0
9	37.39728339543838	39.0	37.0	39.0	34.0	39.0
10	37.27429500953486	39.0	37.0	39.0	34.0	39.0
11	37.346478900906945	39.0	37.0	39.0	34.0	39.0
12	37.25263455337256	39.0	37.0	39.0	34.0	39.0
13	37.276550283930355	39.0	37.0	39.0	34.0	39.0
14	38.483415988951045	40.0	38.0	41.0	34.0	41.0
15	38.47889184051546	40.0	38.0	41.0	34.0	41.0
16	38.43818130441747	40.0	38.0	41.0	34.0	41.0
17	38.39829732449659	40.0	38.0	41.0	34.0	41.0
18	38.35151303601488	40.0	38.0	41.0	34.0	41.0
19	38.381655892726	40.0	38.0	41.0	34.0	41.0
20	38.357857270218894	40.0	38.0	41.0	34.0	41.0
21	38.297483914642584	40.0	38.0	41.0	34.0	41.0
22	38.2680792164187	40.0	38.0	41.0	34.0	41.0
23	38.22039810692947	40.0	38.0	41.0	34.0	41.0
24	38.18547573067868	40.0	38.0	41.0	33.0	41.0
25	38.1648783134023	40.0	38.0	41.0	33.0	41.0
26	37.9870221613764	40.0	37.0	41.0	33.0	41.0
27	37.86232550900448	40.0	37.0	41.0	33.0	41.0
28	37.79768262056085	40.0	37.0	41.0	33.0	41.0
29	37.72651190271117	40.0	37.0	41.0	33.0	41.0
30	37.658319507028	40.0	37.0	41.0	32.0	41.0
31	37.570375894176635	40.0	37.0	41.0	32.0	41.0
32	37.48858460943332	40.0	37.0	41.0	32.0	41.0
33	37.392883910411584	39.0	36.0	41.0	32.0	41.0
34	37.30620582004346	39.0	36.0	41.0	31.0	41.0
35	37.18909701382026	39.0	36.0	41.0	31.0	41.0
36	37.08080228836686	39.0	36.0	41.0	31.0	41.0
37	36.99019314517469	39.0	36.0	41.0	31.0	41.0
38	36.90156426134286	39.0	36.0	41.0	31.0	41.0
39	36.77932007821307	39.0	35.0	41.0	30.0	41.0
40	36.664716628738766	39.0	35.0	40.0	30.0	41.0
41	36.52676636717227	39.0	35.0	40.0	30.0	41.0
42	36.47800408593766	39.0	35.0	40.0	30.0	41.0
43	35.26698746717197	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	5.0
11	1.0
12	1.0
13	1.0
14	1.0
15	4.0
16	8.0
17	18.0
18	52.0
19	95.0
20	194.0
21	449.0
22	833.0
23	1485.0
24	2400.0
25	3939.0
26	6089.0
27	8917.0
28	12549.0
29	17408.0
30	22786.0
31	29718.0
32	37874.0
33	48430.0
34	63248.0
35	83373.0
36	118220.0
37	181396.0
38	322440.0
39	361627.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.69875578362663	19.512996727018866	11.714129426306547	25.07411806304795
2	19.398457498088494	21.320767261726672	33.75945553067324	25.52131970951159
3	20.78123913917272	22.43064936791874	29.1741086944039	27.614002798504643
4	14.428316273334724	16.000888510113604	34.43082465222687	35.139970564324805
5	15.323021780586357	35.773487341753025	33.66153808958237	15.241952788078251
6	35.2118220199401	34.67229389738615	15.571366401624703	14.544517681049046
7	30.177535804842076	29.710992441619748	20.88421867019653	19.227253083341644
8	27.39799511017223	32.88960715159977	20.027441060651395	19.684956677576604
9	26.50291183501516	13.43826214674923	20.078817495791665	39.98000852244395
10	17.16501808752731	26.345004850539905	33.49199585361947	22.997981208313313
11	35.92754109359275	22.042606175447503	20.740440205384854	21.28941252557489
12	21.29092359719666	25.202936918804074	28.9606698278285	24.545469656170763
13	30.630857291373896	19.634789099733748	24.812702672481272	24.921650936411083
14	23.077161361294202	21.11291936015183	25.025310449664694	30.78460882888927
15	25.036190165341456	27.738666207300895	22.761876267411324	24.46326735994633
16	25.808347764067317	25.756140239535075	24.04084728807976	24.394664708317844
17	23.693376368653123	26.135645877343293	25.390763121390425	24.780214632613156
18	23.311377462668975	24.785276722546097	26.964393108304545	24.938952706480382
19	25.35918172449538	25.38358553118701	26.445717774131055	22.811514970186558
20	25.192057203127316	25.049034274126527	26.127108322680275	23.631800200065882
21	24.340417237096204	25.280303785838843	25.9966272881402	24.382651688924753
22	25.321556041113237	24.422392872577376	26.24436748052984	24.011683605779545
23	23.286520334490817	25.137583071162407	26.58964734610491	24.98624924824187
24	24.156595374307553	25.735060790411346	25.914651652659032	24.193692182622073
25	24.43931687474123	25.296698912935074	26.239154283434722	24.02482992888897
26	25.004231000540965	25.507795618496726	25.158133645218516	24.329839735743793
27	24.74742437842069	25.252953389484755	25.250309014146655	24.749313217947904
28	24.250584029181816	25.586598003572174	26.22691460329837	23.935903363947645
29	24.423752837036968	25.92583358266015	26.6469169605701	23.003496619732783
30	24.632885149490317	25.424611125718137	25.505151243158625	24.437352481632924
31	23.709847049330442	25.134787588662128	26.35996445959546	24.795400902411973
32	23.39645079497478	25.738687362303597	26.13194375186995	24.73291809085167
33	23.927516916446805	24.957463333847095	26.415269680952335	24.699750068753758
34	24.81444040484631	25.15881362744831	26.372808568380524	23.653937399324853
35	25.020248359731756	25.111743746430093	25.5558476960691	24.312160197769053
36	23.86526076562977	25.23542495867219	25.292467912394113	25.606846363303926
37	23.831110546977705	24.76004182646249	27.370266945912704	24.0385806806471
38	23.719140139804345	24.41377976433327	26.455086418186045	25.411993677676335
39	23.140248601503213	25.314227343747643	27.65472617871142	23.890797876037727
40	24.357039024935702	24.1921811110003	27.0950252500068	24.355754614057197
41	22.83123445485069	24.795174241668708	27.76163449595184	24.611956807528763
42	22.7720004472772	25.584709164044956	27.092683088993052	24.550607299684792
43	23.454702605994118	24.07129538125848	27.034657938716975	25.439344074030423
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	207.0
1	214.5
2	222.0
3	533.0
4	844.0
5	844.0
6	1176.0
7	1508.0
8	1422.0
9	1336.0
10	1840.0
11	2344.0
12	2344.0
13	4023.0
14	5702.0
15	7717.5
16	9733.0
17	9446.0
18	9159.0
19	9159.0
20	12041.0
21	14923.0
22	14601.5
23	14280.0
24	16591.0
25	18902.0
26	18902.0
27	20758.5
28	22615.0
29	25625.5
30	28636.0
31	32402.5
32	36169.0
33	36169.0
34	44849.0
35	53529.0
36	58090.5
37	62652.0
38	62442.5
39	62233.0
40	62233.0
41	65851.0
42	69469.0
43	73660.0
44	77851.0
45	77719.5
46	77588.0
47	77588.0
48	78635.5
49	79683.0
50	82488.5
51	85294.0
52	91874.5
53	98455.0
54	98455.0
55	96877.5
56	95300.0
57	87409.5
58	79519.0
59	78123.0
60	76727.0
61	76727.0
62	72571.0
63	68415.0
64	56447.5
65	44480.0
66	39583.0
67	34686.0
68	34686.0
69	31743.5
70	28801.0
71	29042.5
72	29284.0
73	25626.5
74	21969.0
75	21969.0
76	13389.5
77	4810.0
78	3788.5
79	2767.0
80	2216.0
81	1665.0
82	1665.0
83	1238.5
84	812.0
85	640.5
86	469.0
87	387.0
88	305.0
89	305.0
90	243.5
91	182.0
92	105.0
93	28.0
94	17.5
95	7.0
96	7.0
97	5.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1323564.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.415684382195046
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.42595788399372	42.05976761961723
2	9.913086759732668	9.995501066639974
3	2.5915252341663124	3.919605548226688
4	1.03305199022826	2.083280923589856
5	0.5481171764392039	1.3816851285909406
6	0.3743111696344941	1.1322692273413755
7	0.2627967316272886	0.9274353954875657
8	0.21149655579588683	0.8530194883941387
9	0.17110323455982884	0.7763658003306917
>10	1.217907546582788	12.121166702894863
>50	0.1395341610370575	4.834910626900446
>100	0.09030641433036099	9.389150111452327
>500	0.01432238027436745	5.052380010610693
>1k	0.006482761597871584	5.473462349923235
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4632	0.349964187602564	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3854	0.29118350151560485	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2911	0.21993647454902066	No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	2725	0.2058835084665343	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	2677	0.20225693657427973	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	2356	0.17800423704482746	No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC	2342	0.17694648690958656	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	2317	0.17505764738237065	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	1968	0.14868944758243652	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1884	0.14234294677099105	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	1855	0.1401518929194206	No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA	1784	0.13478758866212742	No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT	1770	0.1337298385268865	No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA	1716	0.12964994514810013	No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC	1700	0.12844108785068195	No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG	1608	0.1214901583905274	No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA	1552	0.11725915784956377	No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT	1544	0.11665472920085466	No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG	1529	0.11552142548452511	No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC	1484	0.11212151433553648	No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA	1476	0.11151708568682737	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	1473	0.11129042494356148	No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC	1456	0.11000601406505464	No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA	1439	0.10872160318654783	No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG	1383	0.1044906026455842	No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG	1382	0.10441504906449556	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	7.555358108863644E-5	0.0
2	0.0	0.0	0.0	7.555358108863644E-5	0.0
3	0.0	0.0	0.0	7.555358108863644E-5	0.0
4	0.0	0.0	0.0	7.555358108863644E-5	0.0
5	0.0	0.0	0.0	7.555358108863644E-5	0.0
6	0.0	0.0	0.0	7.555358108863644E-5	0.0
7	0.0	0.0	0.0	7.555358108863644E-5	0.0
8	0.0	0.0	0.0	7.555358108863644E-5	0.0
9	0.0	0.0	0.0	7.555358108863644E-5	0.0
10	0.0	0.0	0.0	1.5110716217727287E-4	0.0
11	0.0	0.0	0.0	2.2666074326590932E-4	0.0
12	0.0	0.0	0.0	3.0221432435454575E-4	0.0
13	0.0	0.0	0.0	3.0221432435454575E-4	0.0
14	0.0	0.0	0.0	3.0221432435454575E-4	0.0
15	0.0	0.0	0.0	3.0221432435454575E-4	0.0
16	0.0	0.0	0.0	4.5332148653181865E-4	0.0
17	0.0	0.0	0.0	6.044286487090915E-4	0.0
18	0.0	0.0	0.0	8.310893919750009E-4	0.0
19	0.0	0.0	0.0	9.821965541522736E-4	0.0
20	0.0	0.0	0.0	0.0011333037163295467	0.0
21	0.0	0.0	0.0	0.0018132859461272746	0.0
22	7.555358108863644E-5	0.0	0.0	0.0030221432435454574	0.0
23	7.555358108863644E-5	0.0	0.0	0.0044576612842295495	0.0
24	7.555358108863644E-5	0.0	0.0	0.007328697365597735	0.0
25	7.555358108863644E-5	0.0	0.0	0.009217536892813646	0.0
26	7.555358108863644E-5	0.0	0.0	0.01465739473119547	0.0
27	7.555358108863644E-5	0.0	0.0	0.027728164259529575	0.0
28	7.555358108863644E-5	0.0	0.0	0.06316279379010006	0.0
29	7.555358108863644E-5	0.0	0.0	0.10622833501062283	0.0
30	7.555358108863644E-5	0.0	0.0	0.16085357413770698	0.0
31	7.555358108863644E-5	0.0	0.0	0.3205738445590844	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATACGG	50	0.007035431	18.5	34
AATGCGT	105	4.7986214E-7	17.619047	35
GGTATCA	1815	0.0	17.429754	1
ATCGGCT	65	0.0015801048	17.076923	25
CGCAAGA	195	0.0	17.076923	2
ATGCGTT	110	7.8097946E-7	16.818182	36
TCTAGCG	135	2.2200766E-8	16.444445	28
ACCCGTT	90	4.446775E-5	16.444445	30
TGCGTTA	125	1.6581544E-7	16.279999	37
GTACTAC	115	1.2424662E-6	16.086956	1
TTAGTCG	70	0.0025927715	15.857143	5
GACGGAC	200	3.6379788E-12	15.725	7
TACGTTA	130	2.5896225E-7	15.653846	19
GTATAGA	120	1.9359413E-6	15.416667	1
CTAGCGG	145	5.3467375E-8	15.310345	29
CGGTTAC	100	1.0935624E-4	14.8	22
CACAACG	100	1.0935624E-4	14.8	17
ACGGTTA	100	1.0935624E-4	14.8	21
TAGACGG	75	0.004104844	14.799999	2
CCCCGTA	75	0.004104844	14.799999	15
>>END_MODULE