FastQCFastQC Report
Fri 10 Feb 2017
ERR1630186.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630186.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1546291
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT83280.5385790902229917No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT60580.39177619219150855No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT58670.3794240540752032No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27100.17525808531511858No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG22220.1436986957823592No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA16160.10450814238717034No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA15920.1029560412626084No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT15620.10101591485690596No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTGCG603.727358E-521.58333229
ACCGTCG1650.021.303038
TAAACGT450.003826396320.5555574
GGTATCA24350.019.6776181
ATACCGT1800.019.5277796
AGGTCGC4750.019.0842110
TACCGTC1950.018.974367
GCGTTAT1601.8189894E-1218.51
TAAGACG609.238783E-418.54
CGCCAAT701.2195429E-418.514
CGAATAC500.007035955318.4999983
ACGAACG1156.409209E-817.6956515
AAGACGG2700.017.129635
CCGTCGT1950.017.0769239
CGTCGTA1950.017.07692310
GTCGCCA5000.017.0212
ACACCGT1107.8118865E-716.8181826
CGGTGAT904.4475535E-516.44444514
ATAACGA1352.2208042E-816.44444312
TCACTCG803.38351E-416.187522
Produced by FastQC (version 0.11.5)