FastQCFastQC Report
Fri 10 Feb 2017
ERR1630185.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630185.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365133
Sequences flagged as poor quality0
Sequence length43
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT156874.296242738947178No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT90552.4799182763540957No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT86352.36489169699808No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39091.0705688064349155No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA22190.6077237609309485No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA14430.39519846193031033No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT12910.3535697951157523No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA12620.34562748368402746No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA8390.22977928590404043No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8130.22265859289628712No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7480.20485686037690376No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6880.18842449189747298No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA5750.157476864594545No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA5750.157476864594545No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA5410.14816518912286755No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA5350.14652195227492448No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5290.14487871542698139No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA5160.14131836892310473No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4530.12406438201970241No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4500.12324276359573086No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4490.12296889078774036No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA4370.1196824170918542No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4310.11803918024391113No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG4310.11803918024391113No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA4000.1095491231962052No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA3960.10845363196424318No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGACT200.001840291937.036
TTAACGG251.2319765E-436.99999635
AGCTAGT352.3838786E-531.7142876
ATGATCG303.5947113E-430.83333215
AACGGCC303.5947113E-430.83333237
TGATCGC250.00549149429.5999989
CGGAATC250.00549149429.59999820
CGTGCGG250.00549149429.59999810
GACACAT250.00549149429.59999826
TGACACA250.00549149429.59999825
GTGACAC250.00549149429.59999824
CTTAGAA250.00549149429.5999982
GACGTCC453.998415E-628.7777777
AATTGCA453.998415E-628.77777717
ACGTCCG453.998415E-628.7777778
TTGCATC453.998415E-628.77777719
AATAGAC453.998415E-628.77777719
GATACAT405.9292026E-527.751
AAGCTAG405.9292026E-527.755
TTTAGAC509.068925E-625.8999983
Produced by FastQC (version 0.11.5)