##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630185.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	365133
Sequences flagged as poor quality	0
Sequence length	43
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.40905094855847	31.0	31.0	34.0	30.0	34.0
2	31.537124828487155	31.0	31.0	34.0	30.0	34.0
3	31.560560124666903	31.0	31.0	34.0	28.0	34.0
4	35.358784333379894	37.0	35.0	37.0	33.0	37.0
5	35.33981042524231	37.0	35.0	37.0	33.0	37.0
6	35.4556312357415	37.0	35.0	37.0	33.0	37.0
7	35.41921984591916	37.0	35.0	37.0	33.0	37.0
8	35.49160169034297	37.0	35.0	37.0	33.0	37.0
9	37.09005485672344	39.0	37.0	39.0	33.0	39.0
10	36.91055040218222	39.0	37.0	39.0	33.0	39.0
11	37.046854159991014	39.0	37.0	39.0	33.0	39.0
12	36.96221102995347	39.0	37.0	39.0	33.0	39.0
13	37.02974258694777	39.0	37.0	39.0	33.0	39.0
14	37.889779888424215	40.0	37.0	41.0	33.0	41.0
15	37.834430193929336	39.0	37.0	41.0	33.0	41.0
16	37.7755037205621	39.0	37.0	41.0	32.0	41.0
17	37.94093385150069	39.0	37.0	41.0	33.0	41.0
18	38.04863159451487	40.0	37.0	41.0	33.0	41.0
19	38.151728274354824	40.0	37.0	41.0	33.0	41.0
20	38.20532518287857	40.0	38.0	41.0	34.0	41.0
21	38.189251587777605	40.0	37.0	41.0	34.0	41.0
22	38.148359638816544	40.0	37.0	41.0	33.0	41.0
23	38.083835205253976	40.0	37.0	41.0	33.0	41.0
24	38.036583929691375	40.0	37.0	41.0	33.0	41.0
25	38.01704310484125	40.0	37.0	41.0	33.0	41.0
26	37.75490848540121	40.0	37.0	41.0	33.0	41.0
27	37.568970758600294	39.0	36.0	41.0	32.0	41.0
28	37.43414317522656	39.0	36.0	41.0	32.0	41.0
29	37.25437580278967	39.0	36.0	40.0	32.0	41.0
30	37.005699293134285	39.0	35.0	40.0	32.0	41.0
31	36.68594457362112	38.0	35.0	40.0	31.0	41.0
32	36.417425979026824	38.0	35.0	40.0	31.0	41.0
33	36.217526216474546	38.0	35.0	40.0	31.0	41.0
34	36.07761829251259	38.0	35.0	40.0	30.0	41.0
35	35.82864600022457	38.0	35.0	40.0	30.0	41.0
36	35.60611612754804	38.0	35.0	40.0	29.0	41.0
37	35.38651669391701	38.0	35.0	40.0	28.0	41.0
38	35.1444268252938	37.0	34.0	40.0	27.0	41.0
39	34.88454070160736	37.0	34.0	40.0	25.0	41.0
40	34.56128588760808	37.0	33.0	40.0	24.0	41.0
41	34.1768506270318	37.0	33.0	40.0	21.0	41.0
42	33.851204903418754	37.0	33.0	40.0	20.0	41.0
43	32.70584143312163	35.0	32.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	10.0
11	9.0
12	6.0
13	2.0
14	5.0
15	3.0
16	3.0
17	6.0
18	14.0
19	39.0
20	84.0
21	180.0
22	302.0
23	586.0
24	989.0
25	1572.0
26	2548.0
27	3686.0
28	5225.0
29	6858.0
30	8799.0
31	10901.0
32	13566.0
33	17410.0
34	22433.0
35	28736.0
36	42206.0
37	65537.0
38	77500.0
39	55912.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.13716097969781	18.80629797909255	15.687434441696588	19.369106599513053
2	17.10226136777558	27.210906710705412	33.90572750203351	21.7811044194855
3	19.04566281327626	29.77709492157652	31.929187446765972	19.248054818381245
4	12.048760314734631	17.71217611117045	34.94562255397348	35.293441020121435
5	10.683230494093934	44.69768550089967	31.618341809696744	13.000742195309654
6	27.02248221880794	37.96671349891683	18.552965631701326	16.4578386505739
7	25.189177642119446	34.62327425896865	22.08482936354698	18.102718735364924
8	33.15723311779543	31.269153979508836	18.483675811279728	17.089937091416004
9	29.272347336450004	12.045747713846735	20.826109938022583	37.85579501168068
10	18.48668841216762	32.04640500858591	28.28667910049215	21.18022747875432
11	31.077716886723465	28.30858892513139	18.546392684309552	22.067301503835587
12	20.51499042814536	34.2061659723991	27.61048713756357	17.668356461891968
13	32.317539088496524	20.675479893627802	26.179501715813142	20.827479302062535
14	23.25864821859432	22.8089490678739	30.39659521325106	23.53580750028072
15	25.615323731352685	29.795992145327865	25.33542572158638	19.253258401733067
16	19.323095967770648	27.252809250327964	29.230170924019465	24.19392385788192
17	18.106826827484777	30.610216003483664	30.754820846102653	20.528136322928905
18	17.392840417053513	25.83168324966519	34.136328406361514	22.63914792691978
19	18.264029819271336	28.05224397685227	37.47182533487798	16.21190086899842
20	19.39512451627215	26.665899822804295	36.93530850402456	17.003667156898995
21	20.218933922707617	26.32684528651204	35.49692851645839	17.957292274321958
22	20.43392407698017	26.71793565632249	34.2551892050294	18.59295106166794
23	18.495726214831308	28.579175259426016	34.45237762678257	18.472720898960105
24	16.90123872671054	29.41941703434091	35.10912462034382	18.570219618604728
25	17.73435980861768	28.570411329570323	35.22606830935577	18.46916055245623
26	18.211993985753136	28.67229201414279	35.12227051512736	17.993443484976705
27	16.93875930140524	29.82502266297486	34.490172074285255	18.746045961334637
28	16.74129700684408	30.06520911558254	36.52121281834291	16.672281059230475
29	17.415571860116724	30.165720436115063	35.91650165829986	16.502206045468363
30	16.487690786644865	31.667365042327045	35.45557372245182	16.389370448576273
31	17.211810490971782	31.24943513733352	35.05024196662586	16.48851240506884
32	16.08975359663465	31.781022257643105	35.19265582678093	16.936568318941315
33	16.331309413282284	31.576165397266205	35.44927464786804	16.64325054158348
34	17.084185762448204	30.98679111447062	34.280659376172515	17.64836374690866
35	16.62846140995199	32.37368301413458	34.234100998814135	16.763754577099306
36	16.169998329375872	32.53198149715309	33.31279287273404	17.98522730073699
37	16.273248377988295	32.28084013222579	34.150022046761045	17.29588944302487
38	16.277630342916144	31.967529639884646	34.22698030580638	17.527859711392836
39	16.162056017944145	31.92234062656621	34.653948013463584	17.261655342026057
40	16.22422514535799	31.302292589275687	34.650661539767704	17.822820725598618
41	15.6293734064026	31.798002371738516	34.46251092067822	18.110113301180668
42	15.148726628379247	31.949454034557274	34.19822366096737	18.703595676096107
43	15.07012513248597	31.719674748653233	34.94781353643741	18.26238658242339
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	27.0
1	63.0
2	99.0
3	475.5
4	852.0
5	852.0
6	1348.0
7	1844.0
8	1846.5
9	1849.0
10	2361.0
11	2873.0
12	2873.0
13	6075.0
14	9277.0
15	18068.5
16	26860.0
17	21571.5
18	16283.0
19	16283.0
20	17117.5
21	17952.0
22	12048.0
23	6144.0
24	6309.5
25	6475.0
26	6475.0
27	6655.5
28	6836.0
29	7697.5
30	8559.0
31	9111.5
32	9664.0
33	9664.0
34	11141.5
35	12619.0
36	13869.5
37	15120.0
38	16768.5
39	18417.0
40	18417.0
41	21348.0
42	24279.0
43	25612.0
44	26945.0
45	28112.5
46	29280.0
47	29280.0
48	31694.5
49	34109.0
50	30850.5
51	27592.0
52	23777.5
53	19963.0
54	19963.0
55	17422.5
56	14882.0
57	12324.5
58	9767.0
59	8137.0
60	6507.0
61	6507.0
62	5510.0
63	4513.0
64	3424.5
65	2336.0
66	1836.5
67	1337.0
68	1337.0
69	1017.5
70	698.0
71	558.0
72	418.0
73	346.0
74	274.0
75	274.0
76	226.0
77	178.0
78	156.0
79	134.0
80	98.0
81	62.0
82	62.0
83	53.5
84	45.0
85	40.0
86	35.0
87	28.0
88	21.0
89	21.0
90	13.5
91	6.0
92	4.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	365133.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.572903313872864
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.43588963677195	18.869761681535866
2	12.024980607899098	6.631272553055958
3	5.2621500266323835	4.3527826172228465
4	2.875998057161211	3.171984654439691
5	1.9239969533496	2.6525090985447237
6	1.3549978160895644	2.2416734264127864
7	1.0069983760911325	1.9436108202831444
8	0.8389986469884566	1.850690285910629
9	0.6739989130747785	1.6725696177479643
>10	4.99099195127022	27.087576532732534
>50	0.4419992872092641	8.38463064722988
>100	0.1509997564900427	7.393110643039061
>500	0.009999983873512763	1.7533580941739229
>1k	0.004999991936756381	2.7914762298186497
>5k	0.0019999967747025525	4.877638730293551
>10k+	9.999983873512763E-4	4.325354367558787
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	15687	4.296242738947178	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9055	2.4799182763540957	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8635	2.36489169699808	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3909	1.0705688064349155	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	2219	0.6077237609309485	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	1443	0.39519846193031033	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1291	0.3535697951157523	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1262	0.34562748368402746	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	839	0.22977928590404043	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	813	0.22265859289628712	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	748	0.20485686037690376	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	688	0.18842449189747298	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	575	0.157476864594545	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	575	0.157476864594545	No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA	541	0.14816518912286755	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	535	0.14652195227492448	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	529	0.14487871542698139	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	516	0.14131836892310473	No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	453	0.12406438201970241	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	450	0.12324276359573086	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	449	0.12296889078774036	No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA	437	0.1196824170918542	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	431	0.11803918024391113	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	431	0.11803918024391113	No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA	400	0.1095491231962052	No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	396	0.10845363196424318	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.73872807990513E-4	0.0
10	0.0	0.0	0.0	2.73872807990513E-4	0.0
11	0.0	0.0	0.0	2.73872807990513E-4	0.0
12	0.0	0.0	0.0	8.216184239715392E-4	0.0
13	0.0	0.0	0.0	0.0016432368479430783	0.0
14	0.0	0.0	0.0	0.002190982463924104	0.0
15	0.0	0.0	0.0	0.0030126008878956436	0.0
16	0.0	0.0	0.0	0.004655837735838722	0.0
17	0.0	0.0	0.0	0.006846820199762826	0.0
18	0.0	0.0	0.0	0.006846820199762826	0.0
19	0.0	0.0	0.0	0.009311675471677444	0.0
20	0.0	0.0	0.0	0.010954912319620522	0.0
21	0.0	0.0	0.0	0.013967513207516165	0.0
22	0.0	0.0	0.0	0.018349478135364375	0.0
23	0.0	0.0	0.0	0.02848277203101336	0.0
24	0.0	0.0	0.0	0.04025930277460542	0.0
25	0.0	0.0	0.0	0.041628666814557985	0.0
26	0.0	0.0	0.0	0.04601063174240619	0.0
27	0.0	0.0	0.0	0.0545006887901121	0.0
28	0.0	0.0	0.0	0.07668438623734365	0.0
29	0.0	0.0	0.0	0.12269501797974984	0.0
30	0.0	0.0	0.0	0.24182968945562303	0.0
31	0.0	0.0	0.0	0.5110466597102974	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCTGACT	20	0.0018402919	37.0	36
TTAACGG	25	1.2319765E-4	36.999996	35
AGCTAGT	35	2.3838786E-5	31.714287	6
ATGATCG	30	3.5947113E-4	30.833332	15
AACGGCC	30	3.5947113E-4	30.833332	37
TGATCGC	25	0.005491494	29.599998	9
CGGAATC	25	0.005491494	29.599998	20
CGTGCGG	25	0.005491494	29.599998	10
GACACAT	25	0.005491494	29.599998	26
TGACACA	25	0.005491494	29.599998	25
GTGACAC	25	0.005491494	29.599998	24
CTTAGAA	25	0.005491494	29.599998	2
GACGTCC	45	3.998415E-6	28.777777	7
AATTGCA	45	3.998415E-6	28.777777	17
ACGTCCG	45	3.998415E-6	28.777777	8
TTGCATC	45	3.998415E-6	28.777777	19
AATAGAC	45	3.998415E-6	28.777777	19
GATACAT	40	5.9292026E-5	27.75	1
AAGCTAG	40	5.9292026E-5	27.75	5
TTTAGAC	50	9.068925E-6	25.899998	3
>>END_MODULE