FastQCFastQC Report
Fri 10 Feb 2017
ERR1630184.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630184.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141321
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31312.215523524458502No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT31052.197125692572229No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT17801.2595438752910042No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11240.7953524246219599No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4530.32054684017237356No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3850.27242943370058237No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA3760.26606095343225705No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2670.18893158129365062No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA2650.187516363456245No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2580.18256310102532533No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC2420.17124135832608034No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA2380.1684109226512691No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT2320.16416526913905222No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2260.15991961562683535No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2250.15921200670813257No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA2220.1570891799520241No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA2150.15213591752110445No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2120.150013090764996No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2090.14789026400888755No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2030.1436446104966707No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG1860.1316152588787229No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1820.12878482320391169No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA1800.12736960536650604No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT1790.12666199644780324No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1780.1259543875291004No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1730.12241634293558637No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC1720.12170873401688355No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC1620.11463264482985544No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC1580.11180220915504419No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA1580.11180220915504419No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA1570.11109460023634138No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC1520.10755655564282732No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1490.10543372888671888No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA1480.10472611996801608No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCAC200.00183647937.021
TTGATCA200.00183647937.020
ACGCCTA251.2281534E-437.06
ACGTTTA200.00183647937.026
TTTGTTA200.00183647937.036
ATCACGT200.00183647937.023
TATTGTA200.00183647937.031
CTAACGC251.2281534E-437.03
GCCTACC251.2281534E-437.08
TTTTGCG352.373621E-531.7142872
CGCCTAC303.583662E-430.8333347
CTACCAG303.583662E-430.83333410
AGTTTTT250.005480244329.615
GATCACG250.005480244329.622
TGACCTT250.005480244329.628
CAGATAA250.005480244329.618
AGAGGGC250.005480244329.68
ATGCCCC250.005480244329.637
TAAGCCG250.005480244329.636
AGGGCAA250.005480244329.610
Produced by FastQC (version 0.11.5)