##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630184.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	141321
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.244747772800928	31.0	31.0	34.0	28.0	34.0
2	31.408608770104937	31.0	31.0	34.0	28.0	34.0
3	31.380997870097154	31.0	31.0	34.0	28.0	34.0
4	35.20454851012942	37.0	35.0	37.0	32.0	37.0
5	35.140573587789504	37.0	35.0	37.0	32.0	37.0
6	35.262360158787445	37.0	35.0	37.0	32.0	37.0
7	35.235831900425275	37.0	35.0	37.0	32.0	37.0
8	35.30500067222847	37.0	35.0	37.0	32.0	37.0
9	36.84504072289327	39.0	37.0	39.0	33.0	39.0
10	36.691263152680776	38.0	35.0	39.0	32.0	39.0
11	36.817076018426135	39.0	37.0	39.0	32.0	39.0
12	36.764168099574725	38.0	37.0	39.0	32.0	39.0
13	36.80917910289341	39.0	37.0	39.0	32.0	39.0
14	37.67524996285053	39.0	37.0	40.0	32.0	41.0
15	37.71074362621267	39.0	37.0	40.0	32.0	41.0
16	37.52644688333652	39.0	36.0	40.0	32.0	41.0
17	37.64854480225869	39.0	37.0	40.0	32.0	41.0
18	37.677273724358024	39.0	37.0	40.0	32.0	41.0
19	37.73348617685977	39.0	37.0	40.0	32.0	41.0
20	37.75941296764105	39.0	37.0	41.0	33.0	41.0
21	37.72427310873826	39.0	37.0	41.0	33.0	41.0
22	37.67403995159955	39.0	37.0	41.0	32.0	41.0
23	37.58040206338761	39.0	36.0	40.0	32.0	41.0
24	37.50453931121348	39.0	36.0	40.0	32.0	41.0
25	37.45053459853808	39.0	36.0	40.0	32.0	41.0
26	37.14502444788814	39.0	36.0	40.0	31.0	41.0
27	36.90452232859943	39.0	35.0	40.0	31.0	41.0
28	36.77694044055731	39.0	35.0	40.0	31.0	41.0
29	36.62326901168262	38.0	35.0	40.0	30.0	41.0
30	36.39764790795423	38.0	35.0	40.0	30.0	41.0
31	36.17284055448235	38.0	35.0	40.0	30.0	41.0
32	35.932897446239416	38.0	35.0	40.0	30.0	41.0
33	35.74141139674924	38.0	35.0	40.0	30.0	41.0
34	35.581725292065585	38.0	35.0	40.0	29.0	41.0
35	35.32220972113132	38.0	34.0	40.0	27.0	41.0
36	35.047254123590974	37.0	34.0	40.0	26.0	41.0
37	34.82795904359578	37.0	33.0	40.0	25.0	41.0
38	34.55656979500569	37.0	33.0	40.0	24.0	41.0
39	34.27953382724436	37.0	33.0	40.0	23.0	41.0
40	33.942145894806856	37.0	33.0	40.0	21.0	41.0
41	33.6033710488887	37.0	33.0	40.0	20.0	41.0
42	33.28871859100912	36.0	33.0	40.0	18.0	41.0
43	32.063430063472524	35.0	31.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	1.0
12	1.0
13	1.0
14	0.0
15	0.0
16	5.0
17	4.0
18	15.0
19	23.0
20	59.0
21	130.0
22	214.0
23	370.0
24	575.0
25	856.0
26	1268.0
27	1831.0
28	2451.0
29	3192.0
30	3964.0
31	4852.0
32	5809.0
33	7554.0
34	9363.0
35	12076.0
36	17023.0
37	24787.0
38	30060.0
39	14833.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.560964046390836	20.621139108837326	14.579574160952724	20.238322683819106
2	19.3049865200501	25.18026337203954	32.53232003736175	22.98243007054861
3	22.533098407172325	25.953679920181717	31.251547894509663	20.2616737781363
4	14.921349268686182	18.893158129365062	32.91301363562386	33.27247896632489
5	13.2393628689296	40.0966593782948	31.783669801374177	14.88030795140142
6	29.31057663050785	34.02254442014987	18.1721046412069	18.49477430813538
7	25.082613341258554	34.08693683175183	21.304689324304242	19.525760502685376
8	30.07196382703208	31.6131360519668	20.27228791191684	18.042612209084282
9	27.672461983710843	12.954196474692367	21.904034078445527	37.46930746315126
10	21.435596974264264	28.474890497519826	28.08499798331458	22.004514544901323
11	31.790038281642502	25.07978290558374	19.236348454935925	23.89383035783783
12	20.040899795501023	30.76754339411694	28.229350202729954	20.962206607652085
13	32.24927646988063	19.865412783662727	27.184211829805903	20.701098916650746
14	22.334260301016833	23.77282923273965	28.961725433587365	24.931185032656153
15	28.916438462790385	26.240261532256355	24.885898061859173	19.95740194309409
16	20.776105462033243	26.323759384663287	29.41813318615068	23.482001967152794
17	19.800312763142067	29.43936145371176	28.490457893731293	22.26986788941488
18	19.293664777350852	24.972226349940915	31.821880682984126	23.912228189724104
19	19.98995195335442	27.961166422541588	33.1981800298611	18.850701594242896
20	21.622405728801805	24.080639112375373	34.62684243672207	19.67011272210075
21	23.089279017272734	24.897219804558414	32.15941013720537	19.854091040963482
22	22.89680939138557	25.898486424522893	31.074645664833962	20.130058519257577
23	21.720763368501498	26.75823126074681	31.269238117477233	20.251767253274462
24	20.26450421381111	27.331394484896087	31.979677471854856	20.424423829437945
25	20.71737392178091	27.499097798628654	31.94358941700101	19.839938862589424
26	21.85520906305503	26.772383439120865	31.475859921738454	19.89654757608565
27	20.511459726438392	27.37668145569307	32.073081849123625	20.038776968744916
28	19.31630826274934	28.825156912277723	32.00444378400945	19.854091040963482
29	19.762101881532114	28.56263400343898	31.5883697398122	20.086894375216705
30	18.903772263145605	30.340855216139147	32.99651148803079	17.758861032684457
31	20.32748140757566	28.446586140771718	31.466661005795316	19.759271445857305
32	18.088606788799964	29.573099539346593	31.87565896080554	20.462634711047897
33	19.326214787611182	28.735290579602463	32.670303776508796	19.268190856277553
34	18.09568287798699	29.49172451369577	31.45463165417737	20.957960954139864
35	19.297202821944367	30.151216025926793	31.185032656151595	19.36654849597724
36	18.279661196849727	30.38897262261094	31.171588086696246	20.159778093843094
37	18.905895089901716	30.72508685899477	30.625314001457678	19.743704049645842
38	18.792677662909263	29.624047381493195	30.87156190516625	20.71171305043129
39	19.087042973089634	28.598722058292825	32.122614473432826	20.19162049518472
40	18.757297216974123	29.85331267115291	31.310279434761995	20.079110677110975
41	18.309380771435244	28.767132980944094	31.921653540521223	21.001832707099442
42	17.451051153048734	29.302085323483418	32.027794878326645	21.219068645141203
43	18.022799159360606	29.92973443437281	31.17300330453365	20.874463101732935
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9.0
1	34.0
2	59.0
3	156.0
4	253.0
5	253.0
6	412.0
7	571.0
8	610.0
9	649.0
10	881.0
11	1113.0
12	1113.0
13	2180.0
14	3247.0
15	5027.0
16	6807.0
17	5653.5
18	4500.0
19	4500.0
20	5181.5
21	5863.0
22	4134.0
23	2405.0
24	2341.5
25	2278.0
26	2278.0
27	2470.5
28	2663.0
29	2703.5
30	2744.0
31	2853.5
32	2963.0
33	2963.0
34	3350.5
35	3738.0
36	3901.0
37	4064.0
38	4570.5
39	5077.0
40	5077.0
41	5678.5
42	6280.0
43	7341.5
44	8403.0
45	9761.0
46	11119.0
47	11119.0
48	13116.0
49	15113.0
50	13458.0
51	11803.0
52	10598.0
53	9393.0
54	9393.0
55	9012.5
56	8632.0
57	7852.0
58	7072.0
59	6008.5
60	4945.0
61	4945.0
62	4140.5
63	3336.0
64	2744.0
65	2152.0
66	1791.0
67	1430.0
68	1430.0
69	1323.5
70	1217.0
71	925.0
72	633.0
73	504.0
74	375.0
75	375.0
76	299.0
77	223.0
78	166.5
79	110.0
80	78.0
81	46.0
82	46.0
83	34.5
84	23.0
85	14.5
86	6.0
87	6.0
88	6.0
89	6.0
90	3.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	141321.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.754176661642646
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.10758085473736	30.255942145894803
2	10.453712109076022	8.311574359083222
3	3.962193624178993	4.725412359097374
4	2.1519730869867035	3.4219967308467956
5	1.2655524109574412	2.5155497059884944
6	0.9807586194620956	2.339355085231494
7	0.7102045175415176	1.976351709936952
8	0.5446681262348481	1.732226632984482
9	0.4503301828020149	1.6112255078863011
>10	3.00635446147274	22.554326674733407
>50	0.2580943735426568	7.197090312126295
>100	0.10145778822021678	6.89140325924668
>500	0.0	0.0
>1k	0.007119844787383634	6.467545516943696
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3131	2.215523524458502	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3105	2.197125692572229	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1780	1.2595438752910042	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1124	0.7953524246219599	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	453	0.32054684017237356	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	385	0.27242943370058237	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	376	0.26606095343225705	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	267	0.18893158129365062	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	265	0.187516363456245	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	258	0.18256310102532533	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	242	0.17124135832608034	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	238	0.1684109226512691	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT	232	0.16416526913905222	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	226	0.15991961562683535	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	225	0.15921200670813257	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	222	0.1570891799520241	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	215	0.15213591752110445	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	212	0.150013090764996	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	209	0.14789026400888755	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	203	0.1436446104966707	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	186	0.1316152588787229	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	182	0.12878482320391169	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	180	0.12736960536650604	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	179	0.12666199644780324	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	178	0.1259543875291004	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	173	0.12241634293558637	No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC	172	0.12170873401688355	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	162	0.11463264482985544	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	158	0.11180220915504419	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	158	0.11180220915504419	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	157	0.11109460023634138	No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC	152	0.10755655564282732	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	149	0.10543372888671888	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	148	0.10472611996801608	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	7.076089187028113E-4	0.0
15	0.0	0.0	0.0	7.076089187028113E-4	0.0
16	0.0	0.0	0.0	0.0035380445935140566	0.0
17	0.0	0.0	0.0	0.007076089187028113	0.0
18	0.0	0.0	0.0	0.008491307024433736	0.0
19	0.0	0.0	0.0	0.012029351617947793	0.0
20	0.0	0.0	0.0	0.014152178374056227	0.0
21	0.0	0.0	0.0	0.017690222967570283	0.0
22	0.0	0.0	0.0	0.027596747829409643	0.0
23	0.0	0.0	0.0	0.037503272691249	0.0
24	0.0	0.0	0.0	0.05802393133363053	0.0
25	0.0	0.0	0.0	0.06580762943936146	0.0
26	0.0	0.0	0.0	0.08208263456952611	0.0
27	0.0	0.0	0.0	0.0941119861874739	0.0
28	0.0	0.0	0.0	0.12383156077299198	0.0
29	0.0	0.0	0.0	0.1811478831879197	0.0
30	0.0	0.0	0.0	0.29648813693647796	0.0
31	0.0	0.0	0.0	0.5646719171248434	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCAC	20	0.001836479	37.0	21
TTGATCA	20	0.001836479	37.0	20
ACGCCTA	25	1.2281534E-4	37.0	6
ACGTTTA	20	0.001836479	37.0	26
TTTGTTA	20	0.001836479	37.0	36
ATCACGT	20	0.001836479	37.0	23
TATTGTA	20	0.001836479	37.0	31
CTAACGC	25	1.2281534E-4	37.0	3
GCCTACC	25	1.2281534E-4	37.0	8
TTTTGCG	35	2.373621E-5	31.714287	2
CGCCTAC	30	3.583662E-4	30.833334	7
CTACCAG	30	3.583662E-4	30.833334	10
AGTTTTT	25	0.0054802443	29.6	15
GATCACG	25	0.0054802443	29.6	22
TGACCTT	25	0.0054802443	29.6	28
CAGATAA	25	0.0054802443	29.6	18
AGAGGGC	25	0.0054802443	29.6	8
ATGCCCC	25	0.0054802443	29.6	37
TAAGCCG	25	0.0054802443	29.6	36
AGGGCAA	25	0.0054802443	29.6	10
>>END_MODULE