FastQCFastQC Report
Fri 10 Feb 2017
ERR1630183.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630183.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99182
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC5240.5283216712709968No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT2860.28835877477768146No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT1770.17845980117360005No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG1670.16837732653102377No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT1600.16131959428122036No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG1480.14922062471012884No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG1430.14417938738884073No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG1420.14317113992458308No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT1350.1361134076747797No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT1340.13510516021052207No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC1340.13510516021052207No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT1330.13409691274626445No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG1290.13006392288923393No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG1280.1290556754249763No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG1210.12199794317517292No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC1160.11695670585388479No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG1140.11494021092536952No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG1080.10889072613982376No Hit
TGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGA1040.10485773628279324No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT1030.10384948881853563No Hit
GCCGCAGCCTTTGTGAACCAACACCTGTGCGGCTCACACCTGG1020.102841241354278No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCAGT200.001833837137.06
GAGCACT200.001833837137.06
GGTATCA551.8198989E-830.2727281
GCACTGC250.005472453329.68
CTTATAC451.3113375E-424.66666837
GCTGCAT851.9226718E-923.94117712
TGCATCA851.9226718E-923.94117714
AGATGTC551.8800298E-523.5454545
CTGCATC952.728484E-1023.36842213
GCTGGGG400.001918835623.1250023
TTAGATG502.6780504E-422.23
CTTAGAT502.6780504E-422.22
TCAGAGC450.003801571420.5555573
GCATCAG1001.2543751E-820.3515
AGCCCTT555.097687E-420.1818185
ATGTCCG555.097687E-420.1818187
CAGGACA1152.9522198E-919.3043484
GACAGGC1103.747118E-818.57
CCTTAGA609.1559277E-418.51
GATGTCC609.1559277E-418.56
Produced by FastQC (version 0.11.5)