##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630183.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	99182
Sequences flagged as poor quality	0
Sequence length	43
%GC	54
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.727198483595814	31.0	31.0	34.0	30.0	34.0
2	31.96140428706822	33.0	31.0	34.0	30.0	34.0
3	32.06931701316771	33.0	31.0	34.0	30.0	34.0
4	35.73246153535924	37.0	35.0	37.0	33.0	37.0
5	35.64711338750983	37.0	35.0	37.0	33.0	37.0
6	35.71744873061644	37.0	35.0	37.0	33.0	37.0
7	35.690730172813616	37.0	35.0	37.0	33.0	37.0
8	35.675646790748324	37.0	35.0	37.0	33.0	37.0
9	37.431217357988345	39.0	37.0	39.0	34.0	39.0
10	37.29541650702748	39.0	37.0	39.0	34.0	39.0
11	37.39202677905265	39.0	37.0	39.0	34.0	39.0
12	37.29355124921861	39.0	37.0	39.0	34.0	39.0
13	37.30315984755298	39.0	37.0	39.0	34.0	39.0
14	38.51049585610292	40.0	38.0	41.0	34.0	41.0
15	38.52491379484181	40.0	38.0	41.0	34.0	41.0
16	38.46889556572765	40.0	38.0	41.0	34.0	41.0
17	38.42009638845758	40.0	38.0	41.0	34.0	41.0
18	38.3124458066988	40.0	38.0	41.0	33.0	41.0
19	38.33147143634934	40.0	38.0	41.0	34.0	41.0
20	38.291988465649005	40.0	38.0	41.0	34.0	41.0
21	38.230989494061426	40.0	38.0	41.0	33.0	41.0
22	38.19350285334033	40.0	38.0	41.0	33.0	41.0
23	38.1246798814301	40.0	38.0	41.0	33.0	41.0
24	38.07969187957492	40.0	37.0	41.0	33.0	41.0
25	38.047649775160814	40.0	37.0	41.0	33.0	41.0
26	37.83624044685528	40.0	37.0	41.0	33.0	41.0
27	37.71107660664234	40.0	37.0	41.0	32.0	41.0
28	37.620213345163435	40.0	37.0	41.0	32.0	41.0
29	37.532374826077316	40.0	37.0	41.0	32.0	41.0
30	37.47830251456917	40.0	37.0	41.0	32.0	41.0
31	37.44711742049969	40.0	37.0	41.0	31.0	41.0
32	37.361275231392796	40.0	37.0	41.0	31.0	41.0
33	37.24369341211107	40.0	36.0	41.0	31.0	41.0
34	37.1921719666875	39.0	36.0	41.0	31.0	41.0
35	36.98231533947692	39.0	36.0	41.0	30.0	41.0
36	36.93558306950858	39.0	36.0	41.0	30.0	41.0
37	36.832005807505396	39.0	35.0	41.0	30.0	41.0
38	36.72711782379867	39.0	35.0	41.0	30.0	41.0
39	36.66097679014337	39.0	35.0	41.0	30.0	41.0
40	36.545068661652316	39.0	35.0	40.0	30.0	41.0
41	36.42169950192575	39.0	35.0	40.0	30.0	41.0
42	36.37030912867254	39.0	35.0	40.0	30.0	41.0
43	34.96540702950132	38.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	1.0
17	3.0
18	10.0
19	12.0
20	28.0
21	44.0
22	90.0
23	122.0
24	222.0
25	364.0
26	527.0
27	699.0
28	1016.0
29	1356.0
30	1745.0
31	2297.0
32	2894.0
33	3750.0
34	5048.0
35	6409.0
36	8716.0
37	12674.0
38	23400.0
39	27754.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.950515214454235	16.546349135931923	11.275231392793048	28.227904256820796
2	21.65211429493255	18.411606944808533	31.678127079510393	28.258151680748522
3	21.768062753322177	20.219394648222462	27.492891855376982	30.51965074307838
4	16.672380068964127	14.013631505716765	31.014700248028877	38.29928817729024
5	17.356980097195056	34.492145752253435	31.882801314754694	16.26807283579682
6	39.60093565364683	30.83422395192676	14.130588211570647	15.43425218285576
7	33.07152507511444	27.400133088665278	19.125446149502935	20.402895686717347
8	29.99637030912867	30.8594301385332	18.256336835312858	20.887862717025264
9	28.73908572119941	12.795668568893548	16.80748522917465	41.65776048073239
10	19.515637918170636	24.38345667560646	30.414793006795588	25.686112399427312
11	39.698735657679826	19.205097699179284	18.89859047004497	22.197576173095925
12	24.972273194732917	22.12397410820512	26.459438204512914	26.444314492549054
13	32.81139722933597	16.83269141578109	22.503075154765988	27.85283620011696
14	24.53368554778085	18.13333064467343	22.70472464761751	34.62825915992821
15	28.87217438648142	24.29473089875179	19.462200802564983	27.37089391220181
16	27.96475166864955	23.02736383617995	20.44423383275191	28.56365066241858
17	26.777036155754068	23.75330201044544	22.42140711016112	27.04825472363937
18	26.651005222721864	21.713617390252264	23.586941178842935	28.048436208182935
19	27.89619084108004	22.331673085842187	22.368978242019722	27.403157831058056
20	27.64816196487266	22.415357625375574	22.567602992478474	27.368877417273296
21	29.935875461273216	21.946522554495775	21.772095743179207	26.3455062410518
22	28.65540118166603	22.532314331229458	21.98281946320905	26.829465023895466
23	28.06759291000383	22.430481337339437	21.814442136678025	27.68748361597871
24	28.107922808574138	22.77126898025851	22.00399265995846	27.11681555120889
25	28.164384666572563	22.002984412494204	22.52626484644391	27.306366074489326
26	29.248250690649513	22.07658647738501	22.68455969833236	25.990603133633115
27	29.203887802222177	21.92736585267488	21.844689560605755	27.024056784497187
28	27.557419693089475	21.428283357867357	22.989050432538164	28.02524651650501
29	26.983726885926885	21.682361718860278	23.765400980016533	27.568510415196307
30	27.006916577604805	22.819664858542883	22.92553084228993	27.247887721562382
31	27.3164485491319	22.35587102498437	22.76118650561594	27.566493920267792
32	26.82039079671715	22.035248331350445	23.310681373636346	27.83367949829606
33	27.713698050049405	22.204633905345727	22.76521949547297	27.3164485491319
34	28.09784033393156	22.380068964126558	22.83277207557823	26.689318626363658
35	28.485007360206488	21.98181121574479	23.30866487870783	26.224516545340887
36	27.739912484120104	22.784376197293863	21.634974088040167	27.840737230545866
37	26.8143413119316	21.903167913532698	23.56778447702204	27.714706297513665
38	27.44852896694965	21.823516363856346	23.736161803553063	26.991792865640946
39	26.30416809501724	21.822508116392086	24.258433990038515	27.614889798552156
40	26.611683571615817	20.8394668387409	25.788953640781592	26.75989594886169
41	25.35843197354359	20.932225605452604	26.040007259381742	27.669335161622065
42	25.862555705672403	21.534149341614405	25.228368050654353	27.374926902058842
43	24.80389586820189	19.808029682805348	26.394910366800428	28.993164082192337
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	1.0
2	2.0
3	2.5
4	3.0
5	3.0
6	5.0
7	7.0
8	8.5
9	10.0
10	9.0
11	8.0
12	8.0
13	18.0
14	28.0
15	48.5
16	69.0
17	77.0
18	85.0
19	85.0
20	105.0
21	125.0
22	168.0
23	211.0
24	249.5
25	288.0
26	288.0
27	372.0
28	456.0
29	597.0
30	738.0
31	953.0
32	1168.0
33	1168.0
34	1483.5
35	1799.0
36	2180.5
37	2562.0
38	2874.5
39	3187.0
40	3187.0
41	3656.0
42	4125.0
43	4461.0
44	4797.0
45	5144.5
46	5492.0
47	5492.0
48	5821.0
49	6150.0
50	6727.5
51	7305.0
52	8034.0
53	8763.0
54	8763.0
55	8953.0
56	9143.0
57	8758.5
58	8374.0
59	7691.0
60	7008.0
61	7008.0
62	6865.5
63	6723.0
64	6053.0
65	5383.0
66	4686.5
67	3990.0
68	3990.0
69	3396.0
70	2802.0
71	2549.5
72	2297.0
73	2309.5
74	2322.0
75	2322.0
76	2205.0
77	2088.0
78	1590.0
79	1092.0
80	713.5
81	335.0
82	335.0
83	229.0
84	123.0
85	97.5
86	72.0
87	49.5
88	27.0
89	27.0
90	24.5
91	22.0
92	11.5
93	1.0
94	1.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	99182.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.23718013349196
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.1609066327812	61.523260269000424
2	4.952791855671626	6.759291000383135
3	1.5159798460379144	3.103385694984977
4	0.8230027039406611	2.246375350365994
5	0.5481759482261854	1.870299046197899
6	0.3649581110832016	1.4942227420298038
7	0.23788767564532576	1.136294892218346
8	0.1787851475346858	0.9759835454013833
9	0.1492338834793658	0.9164969450101833
>10	0.9545058289868349	12.798693311286321
>50	0.08274353935489591	3.840414591357303
>100	0.029551264055319968	2.8069609404932345
>500	0.0014775632027659983	0.5283216712709968
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	524	0.5283216712709968	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	286	0.28835877477768146	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	177	0.17845980117360005	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	167	0.16837732653102377	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	160	0.16131959428122036	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	148	0.14922062471012884	No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG	143	0.14417938738884073	No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG	142	0.14317113992458308	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	135	0.1361134076747797	No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT	134	0.13510516021052207	No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC	134	0.13510516021052207	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	133	0.13409691274626445	No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG	129	0.13006392288923393	No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG	128	0.1290556754249763	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	121	0.12199794317517292	No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC	116	0.11695670585388479	No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG	114	0.11494021092536952	No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG	108	0.10889072613982376	No Hit
TGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGA	104	0.10485773628279324	No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT	103	0.10384948881853563	No Hit
GCCGCAGCCTTTGTGAACCAACACCTGTGCGGCTCACACCTGG	102	0.102841241354278	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0010082474642576274	0.0
21	0.0	0.0	0.0	0.0010082474642576274	0.0
22	0.0	0.0	0.0	0.002016494928515255	0.0
23	0.0	0.0	0.0	0.0030247423927728823	0.0
24	0.0	0.0	0.0	0.00403298985703051	0.0
25	0.0	0.0	0.0	0.005041237321288137	0.0
26	0.0	0.0	0.0	0.010082474642576274	0.0
27	0.0	0.0	0.0	0.021173196749410175	0.0
28	0.0	0.0	0.0	0.05646185799842714	0.0
29	0.0	0.0	0.0	0.1068742312113085	0.0
30	0.0	0.0	0.0	0.18955052328043395	0.0
31	0.0	0.0	0.0	0.3609525922042306	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCAGT	20	0.0018338371	37.0	6
GAGCACT	20	0.0018338371	37.0	6
GGTATCA	55	1.8198989E-8	30.272728	1
GCACTGC	25	0.0054724533	29.6	8
CTTATAC	45	1.3113375E-4	24.666668	37
GCTGCAT	85	1.9226718E-9	23.941177	12
TGCATCA	85	1.9226718E-9	23.941177	14
AGATGTC	55	1.8800298E-5	23.545454	5
CTGCATC	95	2.728484E-10	23.368422	13
GCTGGGG	40	0.0019188356	23.125002	3
TTAGATG	50	2.6780504E-4	22.2	3
CTTAGAT	50	2.6780504E-4	22.2	2
TCAGAGC	45	0.0038015714	20.555557	3
GCATCAG	100	1.2543751E-8	20.35	15
AGCCCTT	55	5.097687E-4	20.181818	5
ATGTCCG	55	5.097687E-4	20.181818	7
CAGGACA	115	2.9522198E-9	19.304348	4
GACAGGC	110	3.747118E-8	18.5	7
CCTTAGA	60	9.1559277E-4	18.5	1
GATGTCC	60	9.1559277E-4	18.5	6
>>END_MODULE