FastQCFastQC Report
Fri 10 Feb 2017
ERR1630182.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630182.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2292384
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT73290.3197108337870095No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT70610.308019947792342No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG57470.25069970825132265No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT50610.22077452992168853No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG38510.16799105210994317No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG36100.15747797925652945No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT35450.15464250317573322No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32120.1401161411002694No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA31720.13837123274285634No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC31590.1378041375266971No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC31050.13544851124418944No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC30540.1332237530884878No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA30020.1309553722238508No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA29140.12711657383754205No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT27870.12157648980275555No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA26800.11690885994667559No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG26490.11555655596968047No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT26020.11350628864972012No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC25320.11045269902424724No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG24650.10752997752558036No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT24510.10691925960048577No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC23840.10399653810181889No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA23410.10212076161759984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC3750.018.2533328
GACGGAC4200.017.1785727
GGTATCA29550.017.0287651
GCGCAAG4700.016.5319141
AAGACGG4400.016.3977285
CGCAAGA4650.016.3118272
CGGACCA4200.016.297629
ACGGTAC700.002593549215.8571423
CGAACGA1802.0190782E-1015.41666616
CAAGACG5550.014.9999994
ACGAACG1752.240995E-914.815
GCTTAGA1803.3360266E-914.3888881
TCTTATA15700.014.14012737
ACCTCGC7850.014.14012729
CTAGCGG2650.013.96226429
GCATACG800.0063011413.8750011
CCGTCGT1753.5706762E-813.7428589
CCGATAA1907.1358954E-913.6315799
AACGAAC1907.1358954E-913.63157914
GTAGTTC5050.013.5544553
Produced by FastQC (version 0.11.5)