##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630182.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2292384
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.95534735890671	31.0	31.0	33.0	28.0	34.0
2	31.168785421639654	31.0	31.0	34.0	28.0	34.0
3	31.30590991736114	31.0	31.0	34.0	28.0	34.0
4	35.11248638971481	37.0	35.0	37.0	32.0	37.0
5	34.75751270293284	35.0	35.0	37.0	32.0	37.0
6	34.8257137547636	35.0	35.0	37.0	32.0	37.0
7	34.82921927565364	35.0	35.0	37.0	32.0	37.0
8	34.795310471544035	35.0	35.0	37.0	32.0	37.0
9	36.39441428661167	38.0	35.0	39.0	32.0	39.0
10	36.14874689406312	37.0	35.0	39.0	32.0	39.0
11	36.30728926741767	38.0	35.0	39.0	32.0	39.0
12	36.12907436101456	37.0	35.0	39.0	32.0	39.0
13	36.23560843209515	38.0	35.0	39.0	32.0	39.0
14	37.20715028546701	39.0	36.0	40.0	32.0	41.0
15	37.1468549771766	38.0	36.0	40.0	32.0	41.0
16	37.11435693147396	38.0	36.0	40.0	32.0	41.0
17	37.027838267934165	38.0	36.0	40.0	31.0	41.0
18	36.97749460823317	38.0	36.0	40.0	31.0	41.0
19	36.99091382595586	39.0	36.0	40.0	31.0	41.0
20	36.920889344891606	39.0	36.0	40.0	31.0	41.0
21	36.86825723787987	39.0	36.0	40.0	31.0	41.0
22	36.826317056828174	38.0	36.0	40.0	31.0	41.0
23	36.76690423593953	38.0	36.0	40.0	31.0	41.0
24	36.74176883105099	38.0	36.0	40.0	31.0	41.0
25	36.70556939849519	38.0	36.0	40.0	31.0	41.0
26	36.47906502575485	38.0	35.0	40.0	30.0	41.0
27	36.34427652609685	38.0	35.0	40.0	30.0	41.0
28	36.22996190865056	38.0	35.0	40.0	30.0	41.0
29	36.12342696511579	38.0	35.0	40.0	30.0	41.0
30	36.039023566732276	38.0	35.0	40.0	29.0	41.0
31	35.86709512891383	38.0	34.0	40.0	29.0	41.0
32	35.74354558398593	38.0	34.0	40.0	29.0	41.0
33	35.67349580175049	38.0	34.0	40.0	28.0	41.0
34	35.50882923628851	38.0	34.0	40.0	27.0	41.0
35	35.42537855786814	38.0	34.0	40.0	27.0	41.0
36	35.291668411575024	38.0	34.0	40.0	27.0	41.0
37	35.18887760514818	38.0	34.0	40.0	27.0	41.0
38	35.08450591174952	38.0	33.0	40.0	26.0	41.0
39	34.91912044404428	38.0	33.0	40.0	26.0	41.0
40	34.797364664907796	38.0	33.0	40.0	25.0	41.0
41	34.62446038709047	37.0	33.0	40.0	25.0	41.0
42	34.548313458827145	37.0	33.0	40.0	25.0	41.0
43	33.32832152030375	36.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	3.0
10	6.0
11	4.0
12	9.0
13	11.0
14	18.0
15	48.0
16	81.0
17	177.0
18	300.0
19	657.0
20	1202.0
21	2299.0
22	3722.0
23	6270.0
24	9447.0
25	14475.0
26	20732.0
27	29010.0
28	39303.0
29	50994.0
30	66135.0
31	84188.0
32	105748.0
33	134690.0
34	174151.0
35	230569.0
36	315239.0
37	414913.0
38	434137.0
39	153843.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.572055990619376	20.600257199491885	12.396265198151793	24.43142161173695
2	19.420350168209165	22.186160782835685	33.89519382442034	24.498295224534807
3	20.984137038122757	23.219015662297416	29.191880592431286	26.60496670714854
4	14.81850335720368	16.555603249717326	34.52192128369418	34.10397210938482
5	15.190823177966692	36.396214595809425	33.56632222175691	14.846640004466966
6	35.076060555299634	35.31851557156218	15.548573013945308	14.056850859192876
7	30.16056646704915	30.27119365690914	20.82421618716585	18.74402368887586
8	27.187286248726217	34.08456000390859	19.85827854321091	18.869875204154276
9	26.614999930203666	13.780631866214385	19.908793640158017	39.69557456342393
10	16.824711741139357	27.023875581054487	33.536135307173666	22.615277370632494
11	36.179889582199145	22.221364308946495	20.801706869355222	20.797039239499142
12	21.217780267180366	25.419694082666776	29.51473226126164	23.847793388891215
13	30.456153942794923	19.901857629437302	25.080047670896327	24.56194075687145
14	22.888573642112316	21.183841799628684	25.187315912168295	30.74026864609071
15	24.743323980624538	28.29076629395424	22.84830988176501	24.117599843656212
16	25.595798958638692	26.46214595809428	24.015740818292223	23.926314264974806
17	23.655940714993648	26.509040370199756	25.352689601742117	24.482329313064476
18	23.232451456649496	25.088466853720842	27.252458575875593	24.426623113754065
19	25.115120328880323	25.78512151541801	26.552619456426147	22.547138699275514
20	24.94800173094909	25.495423105378507	26.138378212376285	23.418196951296117
21	24.442370911679717	25.690852841408766	26.0270094364644	23.839766810447117
22	25.348021971886038	24.857310119072544	26.310208062872537	23.48445984616888
23	23.22568993676452	25.539961891201475	26.749227005597664	24.48512116643634
24	24.0159589318369	26.105224953585438	26.04685776902997	23.83195834554769
25	24.34182056758379	25.81823115149992	26.209265114396192	23.630683166520093
26	24.733814230076636	25.935968842916367	25.448572315981966	23.88164461102503
27	24.819401985007747	25.36734683194439	25.289305805659083	24.523945377388777
28	24.131297374261905	25.750179725560812	26.503282172620295	23.615240727556987
29	24.389761924703716	26.136895040272485	26.66189434230914	22.81144869271466
30	24.54846133981043	25.72876097547357	25.69242325893044	24.030354425785557
31	23.67160126750143	25.51487883356366	26.50938935187124	24.304130547063668
32	23.3229685776903	25.97496754470455	26.17855472730572	24.52350915029943
33	23.874926713848986	25.22382811954716	26.587953850663766	24.313291315940084
34	24.80321795999274	25.28311138099027	26.636200566746233	23.277470092270754
35	24.899973128411297	25.380215531080307	25.836901670924245	23.882909669584155
36	23.69048990047043	25.654864106537122	25.597805603249718	25.056840389742728
37	23.729270488713933	25.211395647500595	27.311741837318703	23.747592026466773
38	23.553383726286693	24.684084341890365	26.72017428144674	25.0423576503762
39	23.314593017574715	25.24411267920209	27.65500893393079	23.786285369292404
40	24.21692875190195	24.682732037913368	27.133455825900022	23.966883384284657
41	22.768436701704424	25.350115861914936	27.734009659812664	24.147437776567976
42	22.997804905286372	25.869226098245317	27.10723857782989	24.025730418638414
43	23.433334031296678	24.359444141993663	27.090312966762987	25.116908859946673
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	445.0
1	432.0
2	419.0
3	914.0
4	1409.0
5	1409.0
6	2003.0
7	2597.0
8	2567.5
9	2538.0
10	3284.0
11	4030.0
12	4030.0
13	7154.0
14	10278.0
15	14345.0
16	18412.0
17	17697.0
18	16982.0
19	16982.0
20	21162.5
21	25343.0
22	25286.0
23	25229.0
24	29166.5
25	33104.0
26	33104.0
27	37434.0
28	41764.0
29	47846.5
30	53929.0
31	60735.5
32	67542.0
33	67542.0
34	83548.0
35	99554.0
36	106196.5
37	112839.0
38	112972.0
39	113105.0
40	113105.0
41	117811.0
42	122517.0
43	130097.5
44	137678.0
45	136937.5
46	136197.0
47	136197.0
48	137050.5
49	137904.0
50	142801.5
51	147699.0
52	158326.5
53	168954.0
54	168954.0
55	166456.5
56	163959.0
57	150256.5
58	136554.0
59	132952.0
60	129350.0
61	129350.0
62	119803.5
63	110257.0
64	90506.0
65	70755.0
66	63089.5
67	55424.0
68	55424.0
69	51201.0
70	46978.0
71	46532.5
72	46087.0
73	40066.0
74	34045.0
75	34045.0
76	21072.0
77	8099.0
78	6180.0
79	4261.0
80	3406.5
81	2552.0
82	2552.0
83	1965.5
84	1379.0
85	1127.5
86	876.0
87	770.0
88	664.0
89	664.0
90	572.5
91	481.0
92	305.5
93	130.0
94	87.0
95	44.0
96	44.0
97	29.5
98	15.0
99	10.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2292384.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.98702620187414
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.81407476564058	46.08686725224116
2	10.289255448697485	11.315511179106096
3	2.689507786717319	4.4366410541510914
4	1.0151709499765829	2.2328492650297522
5	0.5360221682926177	1.473713250634578
6	0.32409752265413566	1.069269538208727
7	0.20679417734746708	0.7959697793740124
8	0.14424631406607455	0.6345340680860004
9	0.1095541956419098	0.5421653483658245
>10	0.7006356235992108	7.424527235537112
>50	0.08142182943934234	3.1418827910097757
>100	0.0692372698946744	7.721965071604521
>500	0.01182931323522378	4.612060235233379
>1k	0.007832923628729248	7.4045939480235985
>5k	3.1971116851956113E-4	1.1074499833942628
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7329	0.3197108337870095	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7061	0.308019947792342	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	5747	0.25069970825132265	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5061	0.22077452992168853	No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	3851	0.16799105210994317	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	3610	0.15747797925652945	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	3545	0.15464250317573322	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3212	0.1401161411002694	No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA	3172	0.13837123274285634	No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC	3159	0.1378041375266971	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	3105	0.13544851124418944	No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC	3054	0.1332237530884878	No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA	3002	0.1309553722238508	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	2914	0.12711657383754205	No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT	2787	0.12157648980275555	No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA	2680	0.11690885994667559	No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG	2649	0.11555655596968047	No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT	2602	0.11350628864972012	No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC	2532	0.11045269902424724	No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG	2465	0.10752997752558036	No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT	2451	0.10691925960048577	No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC	2384	0.10399653810181889	No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA	2341	0.10212076161759984	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	4.362270893532672E-5	0.0
2	0.0	4.362270893532672E-5	0.0	4.362270893532672E-5	0.0
3	0.0	4.362270893532672E-5	0.0	4.362270893532672E-5	0.0
4	0.0	4.362270893532672E-5	0.0	8.724541787065344E-5	0.0
5	0.0	8.724541787065344E-5	0.0	8.724541787065344E-5	0.0
6	0.0	8.724541787065344E-5	0.0	8.724541787065344E-5	0.0
7	0.0	8.724541787065344E-5	0.0	8.724541787065344E-5	0.0
8	0.0	8.724541787065344E-5	0.0	8.724541787065344E-5	0.0
9	0.0	8.724541787065344E-5	0.0	1.7449083574130688E-4	0.0
10	0.0	8.724541787065344E-5	0.0	2.1811354467663358E-4	0.0
11	0.0	8.724541787065344E-5	0.0	2.1811354467663358E-4	0.0
12	0.0	8.724541787065344E-5	0.0	2.1811354467663358E-4	0.0
13	0.0	8.724541787065344E-5	0.0	2.1811354467663358E-4	0.0
14	0.0	8.724541787065344E-5	0.0	2.1811354467663358E-4	0.0
15	0.0	8.724541787065344E-5	0.0	3.4898167148261376E-4	0.0
16	0.0	8.724541787065344E-5	0.0	5.670952161592473E-4	0.0
17	0.0	8.724541787065344E-5	0.0	8.288314697712076E-4	0.0
18	4.362270893532672E-5	1.3086812680598015E-4	0.0	8.288314697712076E-4	0.0
19	4.362270893532672E-5	1.3086812680598015E-4	0.0	9.596995965771878E-4	0.0
20	4.362270893532672E-5	1.3086812680598015E-4	0.0	0.0011778131412538214	4.362270893532672E-5
21	4.362270893532672E-5	1.3086812680598015E-4	0.0	0.001395926685930455	1.3086812680598015E-4
22	4.362270893532672E-5	1.3086812680598015E-4	0.0	0.0023992489914429695	1.3086812680598015E-4
23	4.362270893532672E-5	1.7449083574130688E-4	0.0	0.003577062132696791	1.3086812680598015E-4
24	4.362270893532672E-5	1.7449083574130688E-4	0.0	0.006150801959881067	1.3086812680598015E-4
25	4.362270893532672E-5	1.7449083574130688E-4	0.0	0.007241369683264235	1.3086812680598015E-4
26	4.362270893532672E-5	1.7449083574130688E-4	0.0	0.010382204726607759	1.3086812680598015E-4
27	4.362270893532672E-5	2.1811354467663358E-4	0.0	0.021462372796180743	1.3086812680598015E-4
28	4.362270893532672E-5	2.1811354467663358E-4	0.0	0.050951324036461604	1.3086812680598015E-4
29	4.362270893532672E-5	2.1811354467663358E-4	0.0	0.08654745452768821	1.3086812680598015E-4
30	4.362270893532672E-5	2.1811354467663358E-4	0.0	0.13784776023563242	1.3086812680598015E-4
31	4.362270893532672E-5	2.1811354467663358E-4	0.0	0.30405028127922723	1.3086812680598015E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGACC	375	0.0	18.253332	8
GACGGAC	420	0.0	17.178572	7
GGTATCA	2955	0.0	17.028765	1
GCGCAAG	470	0.0	16.531914	1
AAGACGG	440	0.0	16.397728	5
CGCAAGA	465	0.0	16.311827	2
CGGACCA	420	0.0	16.29762	9
ACGGTAC	70	0.0025935492	15.857142	3
CGAACGA	180	2.0190782E-10	15.416666	16
CAAGACG	555	0.0	14.999999	4
ACGAACG	175	2.240995E-9	14.8	15
GCTTAGA	180	3.3360266E-9	14.388888	1
TCTTATA	1570	0.0	14.140127	37
ACCTCGC	785	0.0	14.140127	29
CTAGCGG	265	0.0	13.962264	29
GCATACG	80	0.00630114	13.875001	1
CCGTCGT	175	3.5706762E-8	13.742858	9
CCGATAA	190	7.1358954E-9	13.631579	9
AACGAAC	190	7.1358954E-9	13.631579	14
GTAGTTC	505	0.0	13.554455	3
>>END_MODULE