##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630181.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	48439
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.41138338941762	31.0	30.0	31.0	27.0	34.0
2	30.614566774706333	31.0	30.0	33.0	27.0	34.0
3	30.794958607733438	31.0	31.0	34.0	27.0	34.0
4	34.66097566010859	35.0	35.0	37.0	32.0	37.0
5	33.94267016247239	35.0	33.0	37.0	30.0	37.0
6	34.05305642147856	35.0	33.0	37.0	30.0	37.0
7	34.11833439996697	35.0	33.0	37.0	30.0	37.0
8	34.041557422737874	35.0	33.0	37.0	30.0	37.0
9	35.60443031441607	37.0	35.0	39.0	30.0	39.0
10	35.25070707487768	37.0	34.0	39.0	30.0	39.0
11	35.43877867007989	37.0	35.0	39.0	30.0	39.0
12	35.19928157063523	37.0	34.0	39.0	30.0	39.0
13	35.318028861041725	37.0	34.0	39.0	30.0	39.0
14	36.17110179813786	38.0	35.0	40.0	30.0	41.0
15	36.10708313549	38.0	34.0	40.0	30.0	41.0
16	36.038811701315055	38.0	34.0	40.0	30.0	41.0
17	35.94407398996676	38.0	34.0	40.0	29.0	41.0
18	35.86894857449576	38.0	34.0	40.0	29.0	41.0
19	35.87922954643985	38.0	34.0	40.0	29.0	41.0
20	35.768182662730446	38.0	34.0	40.0	29.0	41.0
21	35.727409731827656	38.0	34.0	40.0	29.0	40.0
22	35.62623092962283	38.0	34.0	40.0	27.0	40.0
23	35.50508887466711	38.0	34.0	40.0	27.0	40.0
24	35.44802741592518	38.0	34.0	40.0	27.0	41.0
25	35.42118953735626	38.0	34.0	40.0	27.0	40.0
26	35.11040690352815	38.0	33.0	39.0	27.0	40.0
27	34.961869567910156	37.0	33.0	39.0	27.0	40.0
28	34.80808852371023	37.0	33.0	39.0	26.0	40.0
29	34.65571130700469	37.0	33.0	39.0	25.0	40.0
30	34.6238361650736	37.0	33.0	39.0	25.0	40.0
31	34.485497223311796	37.0	33.0	39.0	25.0	40.0
32	34.37864117756353	37.0	33.0	39.0	25.0	40.0
33	34.26808976238155	37.0	32.0	39.0	25.0	40.0
34	34.12820248147154	37.0	32.0	39.0	24.0	40.0
35	33.988294556039556	37.0	32.0	39.0	24.0	40.0
36	33.84192489522905	37.0	31.0	39.0	24.0	40.0
37	33.76762526063709	37.0	31.0	39.0	24.0	40.0
38	33.620182084683826	36.0	31.0	39.0	24.0	40.0
39	33.48264827927909	36.0	31.0	39.0	23.0	40.0
40	33.33268647164475	36.0	31.0	39.0	23.0	40.0
41	33.15555647308987	36.0	31.0	39.0	22.0	40.0
42	33.125167736741055	36.0	31.0	39.0	22.0	40.0
43	31.7163236235265	35.0	29.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	0.0
16	4.0
17	17.0
18	11.0
19	30.0
20	62.0
21	97.0
22	143.0
23	238.0
24	353.0
25	470.0
26	687.0
27	969.0
28	1240.0
29	1550.0
30	2086.0
31	2585.0
32	3180.0
33	3920.0
34	4903.0
35	6043.0
36	6909.0
37	6790.0
38	4769.0
39	1381.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.80309254939202	19.701067321786166	11.930469249984517	26.565370878837303
2	21.17302173868164	21.33404901009517	30.797497883936497	26.695431367286695
3	21.53223642106567	21.49714073370631	28.691756642374944	28.27886620285307
4	16.76128739239043	15.706352319412046	32.26119449204154	35.27116579615599
5	16.220400916616775	35.19478106484444	33.276904973265346	15.307913045273436
6	37.8971489915151	33.857016040793575	14.188979954169161	14.056855013522163
7	32.087780507442346	29.3874770329693	19.35836825698301	19.166374202605336
8	29.44734614669997	31.707921303082227	18.582134230681888	20.26259831953591
9	27.83500898036706	13.175333925142962	17.518941348913067	41.47071574557691
10	17.849253700530564	25.704494312434196	31.54070067507587	24.90555131195937
11	40.05037263362167	19.988026177253865	19.275790169078636	20.68581102004583
12	23.61939759284874	23.856809595573814	27.399409566671483	25.124383244905964
13	32.57499122607816	17.931831788434938	22.772972191828895	26.720204793658002
14	24.377051549371373	18.61310101364603	22.84109911435001	34.168748322632595
15	27.114515163401393	25.54346704102067	20.056153099774974	27.28586469580297
16	27.42005408864758	24.600012386713185	21.379466958442578	26.600466566196662
17	26.598402113999047	24.374987097173765	22.69658746051735	26.33002332830983
18	26.83168521232891	22.985610768182664	23.524432791758706	26.658271227729724
19	27.199157703503374	23.5884308098846	22.839034662152397	26.373376824459633
20	26.77181609859824	23.470757034620863	23.049608786308553	26.707818080472347
21	28.12816119242759	22.9938685769731	22.46949771878032	26.408472511818985
22	27.508825533144783	23.423274634075845	22.64291170337951	26.424988129399864
23	26.69956027168191	22.940192819835257	23.1920559879436	27.16819092053924
24	27.42005408864758	23.497594913189783	22.18666776770784	26.895683230454797
25	27.376700592497784	23.136315778608147	23.080575569272693	26.406408059621377
26	27.83088007597184	22.791552261607382	22.678007390738866	26.69956027168191
27	27.01335700571853	22.925741654451993	22.931935011044818	27.12896632878466
28	27.003034744730485	22.56446251987035	23.735006915914862	26.697495819484303
29	26.204091744255663	23.289085241231238	23.792811577447925	26.714011437065178
30	25.931584054171225	24.06531926753236	23.367534424740395	26.635562253556017
31	27.323024835359938	23.53681950494436	22.603687111624932	26.53646854807077
32	26.676851297508207	23.689588967567456	22.2444724292409	27.389087305683436
33	26.76149383761019	23.49140155659696	23.13425132641054	26.612853279382314
34	27.34573380953364	23.0599310472966	23.142509135200974	26.451826007968787
35	26.92458556122133	23.043415429715726	23.763909246681393	26.268089762381553
36	26.55917752224447	23.466628130225644	23.299407502219285	26.674786845310592
37	26.93077891781416	22.73374760007432	23.927000970292532	26.408472511818985
38	26.24950969260307	22.86793699291893	23.99719234501125	26.885360969466753
39	26.57362868762774	22.85761473193088	24.236668799933938	26.33208778050744
40	25.82836144429076	23.19824934453643	24.725943970767357	26.247445240405458
41	25.892359462416646	22.53349573690621	25.33908627345734	26.235058527219802
42	26.695431367286695	22.455046553397057	24.80852205867173	26.04100002064452
43	25.405148743780835	21.82332418092859	25.378310865211915	27.39321621007866
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	1.0
4	1.0
5	1.0
6	1.5
7	2.0
8	4.0
9	6.0
10	6.5
11	7.0
12	7.0
13	14.0
14	21.0
15	36.5
16	52.0
17	50.0
18	48.0
19	48.0
20	68.0
21	88.0
22	85.5
23	83.0
24	108.0
25	133.0
26	133.0
27	195.5
28	258.0
29	310.5
30	363.0
31	455.0
32	547.0
33	547.0
34	722.0
35	897.0
36	1086.0
37	1275.0
38	1554.5
39	1834.0
40	1834.0
41	2017.5
42	2201.0
43	2522.0
44	2843.0
45	3089.5
46	3336.0
47	3336.0
48	3579.0
49	3822.0
50	4004.5
51	4187.0
52	4285.5
53	4384.0
54	4384.0
55	4129.0
56	3874.0
57	3776.0
58	3678.0
59	3627.0
60	3576.0
61	3576.0
62	3450.5
63	3325.0
64	2983.5
65	2642.0
66	2269.0
67	1896.0
68	1896.0
69	1571.5
70	1247.0
71	1013.0
72	779.0
73	624.0
74	469.0
75	469.0
76	356.0
77	243.0
78	195.5
79	148.0
80	116.0
81	84.0
82	84.0
83	64.0
84	44.0
85	31.0
86	18.0
87	18.5
88	19.0
89	19.0
90	12.0
91	5.0
92	3.5
93	2.0
94	1.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	48439.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.19696938417391
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.09353944725534	73.96519333594831
2	4.914698329561923	7.981172195957803
3	1.6628105057079658	4.050455211709573
4	0.7347893519107066	2.386506740436425
5	0.42205893569957537	1.7134953240157724
6	0.2593374183214258	1.2634447449369308
7	0.19831684930461976	1.1271908998947129
8	0.14746637512394803	0.9579058196907451
9	0.08898832981617553	0.6503024422469498
>10	0.4729094098802471	5.679307995623361
>50	0.005085047418067173	0.2250252895394207
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	56	0.1156093230661244	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	53	0.10941596647329631	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0020644521976093642	0.0
26	0.0	0.0	0.0	0.006193356592828093	0.0
27	0.0	0.0	0.0	0.010322260988046821	0.0
28	0.0	0.0	0.0	0.016515617580874914	0.0
29	0.0	0.0	0.0	0.037160139556968556	0.0
30	0.0	0.0	0.0	0.0639980181258903	0.0
31	0.0	0.0	0.0	0.1321249406469993	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGCTGA	50	0.0069403374	18.499998	6
>>END_MODULE