FastQCFastQC Report
Fri 10 Feb 2017
ERR1630180.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630180.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3071741
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT74070.24113361119964216No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT66490.2164570515548023No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT56030.1824047014380444No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT51280.16694115812498514No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA36480.1187600126442952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGAC10500.022.728573
GTTCTAG10850.022.3364051
GTCTAGA7150.020.6992991
GGTATCA25300.020.0355741
AAGACGG7300.019.7671225
CTAGAAC7550.019.602653
GACGGAC7050.018.8936187
CTCTAGT11650.018.579427
CTAGACA14700.018.24834
CTAGCGG5100.018.13725529
ACGGACC7450.018.1275168
CCTCTAG17450.017.70487226
GACATGT12650.017.6956527
TAGAACT9750.017.4564114
TCTAGCG5300.017.45283128
ACTGATC9350.017.4117648
TATACTG5000.017.025
CAAGACG8450.016.8579864
GCGCAAG8300.016.7168661
CTAGTTT13100.016.52290229
Produced by FastQC (version 0.11.5)