##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630180.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3071741
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.8253928309711	31.0	31.0	34.0	30.0	34.0
2	32.01088275346131	33.0	31.0	34.0	30.0	34.0
3	32.170093116574606	34.0	31.0	34.0	30.0	34.0
4	35.814953799815804	37.0	35.0	37.0	35.0	37.0
5	35.72769090883639	37.0	35.0	37.0	35.0	37.0
6	35.78656696642067	37.0	35.0	37.0	35.0	37.0
7	35.76159122790626	37.0	35.0	37.0	33.0	37.0
8	35.74206484205537	37.0	35.0	37.0	33.0	37.0
9	37.447980151972445	39.0	37.0	39.0	35.0	39.0
10	37.35547951471169	39.0	37.0	39.0	34.0	39.0
11	37.439455344705166	39.0	37.0	39.0	34.0	39.0
12	37.31453140092215	39.0	37.0	39.0	34.0	39.0
13	37.35485869414121	39.0	37.0	39.0	34.0	39.0
14	38.57172040220839	40.0	38.0	41.0	34.0	41.0
15	38.55915781962086	40.0	38.0	41.0	34.0	41.0
16	38.550464052796116	40.0	38.0	41.0	34.0	41.0
17	38.51415076987285	40.0	38.0	41.0	34.0	41.0
18	38.49527841051703	40.0	38.0	41.0	34.0	41.0
19	38.50641313834728	40.0	38.0	41.0	34.0	41.0
20	38.45273836563695	40.0	38.0	41.0	34.0	41.0
21	38.4133483259168	40.0	38.0	41.0	34.0	41.0
22	38.37618503643374	40.0	38.0	41.0	34.0	41.0
23	38.324233716319185	40.0	38.0	41.0	34.0	41.0
24	38.30679572268625	40.0	38.0	41.0	34.0	41.0
25	38.28253749258157	40.0	38.0	41.0	34.0	41.0
26	38.13449148219202	40.0	38.0	41.0	33.0	41.0
27	38.03447946946048	40.0	38.0	41.0	33.0	41.0
28	37.94714756224565	40.0	37.0	41.0	33.0	41.0
29	37.88685536964217	40.0	37.0	41.0	33.0	41.0
30	37.822804396594634	40.0	37.0	41.0	33.0	41.0
31	37.72745879291255	40.0	37.0	41.0	33.0	41.0
32	37.62148045684841	40.0	37.0	41.0	32.0	41.0
33	37.5484062621165	40.0	37.0	41.0	32.0	41.0
34	37.47190143960705	40.0	37.0	41.0	32.0	41.0
35	37.3917260602375	40.0	37.0	41.0	32.0	41.0
36	37.2895133411313	39.0	36.0	41.0	31.0	41.0
37	37.23324622746514	39.0	36.0	41.0	31.0	41.0
38	37.128445399530754	39.0	36.0	41.0	31.0	41.0
39	37.04569005004003	39.0	36.0	41.0	31.0	41.0
40	36.94026905263171	39.0	36.0	41.0	30.0	41.0
41	36.82716511580892	39.0	35.0	41.0	30.0	41.0
42	36.750847809108905	39.0	35.0	41.0	30.0	41.0
43	35.59759790945916	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	3.0
11	7.0
12	4.0
13	8.0
14	4.0
15	10.0
16	28.0
17	70.0
18	143.0
19	241.0
20	478.0
21	977.0
22	1791.0
23	3093.0
24	5014.0
25	7914.0
26	12075.0
27	17977.0
28	25385.0
29	35924.0
30	48226.0
31	63391.0
32	83145.0
33	106967.0
34	142372.0
35	189701.0
36	266708.0
37	401716.0
38	725754.0
39	932610.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.516038624350166	18.905369951437965	11.579296561786947	25.999294862424925
2	18.255575584009197	21.04379894007991	34.269686148669436	26.43093932724146
3	19.329722134776336	22.516677024527784	30.269837203071482	27.883763637624398
4	13.750671036392717	15.171396286340547	35.5763067263809	35.50162595088583
5	14.392652245094883	36.732751882401544	34.21929127488287	14.655304597620697
6	34.83001984867865	35.94795264314277	15.484899280245306	13.73712822793328
7	29.90717641884521	30.891992521504907	20.791629242178946	18.409201817470937
8	27.6860256121854	33.57226406783645	19.728486223285103	19.013224096693047
9	26.628872681648613	13.750052494660196	19.325197013680516	40.29587781001068
10	16.56129211414634	26.74883722292993	33.10279089285197	23.58707977007176
11	36.65686657826946	21.478112900794695	21.522159583115894	20.342860937819953
12	21.877104873099654	25.554140143976984	29.278445025150234	23.290309957773133
13	29.975378783562807	19.285447568658945	25.455075802289322	25.28409784548893
14	22.74091467998116	20.057941082923332	25.081834698954108	32.1193095381414
15	24.922739254383753	27.73436953180623	22.17130285398411	25.171588359825908
16	25.028379671332967	26.504838786863864	23.882742718217454	24.584038823585715
17	23.674945250917965	26.490091449767412	25.335371699632226	24.499591599682393
18	24.090800624141163	25.180182834425167	26.381944311060078	24.34707223037359
19	24.374906608337096	25.09661459087859	26.171509902690364	24.356968898093946
20	24.365465708209122	25.25964916964028	26.028463988337556	24.34642113381304
21	25.04967052886295	25.156515474449183	25.891375607513783	23.902438389174087
22	24.959037887634405	24.990388187024884	25.80494253910079	24.24563138623992
23	24.207932895384083	24.981240280349155	26.124240292394447	24.686586531872315
24	24.13562862233502	25.53704885926255	25.841664385115802	24.485658133286627
25	24.757523502144224	24.904768989312576	25.99877398517648	24.338933523366716
26	24.727214957250627	25.453741054340195	25.677750825997375	24.141293162411806
27	24.47227809896733	24.992211257394423	25.765127984423163	24.770382659215084
28	23.95491677195441	24.940351416346626	26.656348956503823	24.448382855195145
29	23.838989029348504	25.245780812900563	26.340502014981084	24.57472814276985
30	23.51243154940472	25.946393266880246	26.47570221577926	24.065472967935772
31	24.592210085420614	25.608409042298813	25.623091269739213	24.17628960254136
32	23.95771648716477	25.423497619102655	25.683806024010487	24.934979869722092
33	23.58125245585484	25.096028603974098	26.710748074137765	24.6119708660333
34	24.653608491080465	24.779139907954477	26.204292614514046	24.362958986451005
35	24.241008600660017	25.119435525325866	26.73477353722205	23.904782336792067
36	24.045809851807167	25.116603255287473	26.354793584485147	24.48279330842021
37	24.114826087225453	24.931138400014845	26.636783504859295	24.317252007900407
38	23.802690396097848	24.729461240384527	27.054657277420198	24.413191086097427
39	24.018431241436044	24.68821427327369	26.959662289235975	24.33369219605429
40	23.70798840136587	25.00051273854143	27.16970603966936	24.121792820423337
41	23.594013948441617	24.43558880778034	27.52256782065936	24.44782942311868
42	23.759425029649307	24.61955614096371	27.49098963747269	24.130029191914293
43	23.14319468991689	24.02930455399723	27.717538685716015	25.109962070369868
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	467.0
1	440.5
2	414.0
3	938.5
4	1463.0
5	1463.0
6	1937.0
7	2411.0
8	2224.0
9	2037.0
10	2815.0
11	3593.0
12	3593.0
13	6114.0
14	8635.0
15	12701.0
16	16767.0
17	16622.0
18	16477.0
19	16477.0
20	20029.0
21	23581.0
22	23822.5
23	24064.0
24	28340.5
25	32617.0
26	32617.0
27	38423.5
28	44230.0
29	52836.5
30	61443.0
31	70974.5
32	80506.0
33	80506.0
34	95045.0
35	109584.0
36	123897.5
37	138211.0
38	150644.0
39	163077.0
40	163077.0
41	175035.5
42	186994.0
43	194070.5
44	201147.0
45	210538.0
46	219929.0
47	219929.0
48	225022.5
49	230116.0
50	232671.0
51	235226.0
52	232353.0
53	229480.0
54	229480.0
55	218612.5
56	207745.0
57	196354.5
58	184964.0
59	178806.0
60	172648.0
61	172648.0
62	158327.0
63	144006.0
64	128063.5
65	112121.0
66	96973.5
67	81826.0
68	81826.0
69	66977.0
70	52128.0
71	44092.0
72	36056.0
73	28533.5
74	21011.0
75	21011.0
76	15747.5
77	10484.0
78	8256.0
79	6028.0
80	4996.5
81	3965.0
82	3965.0
83	2969.5
84	1974.0
85	1810.0
86	1646.0
87	1533.0
88	1420.0
89	1420.0
90	1226.5
91	1033.0
92	595.5
93	158.0
94	100.0
95	42.0
96	42.0
97	24.5
98	7.0
99	8.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3071741.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.30285997757641
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.90119618919515	39.88660347734327
2	10.593291030130372	10.445590887204677
3	3.2633027987543133	4.826704828542513
4	1.4990868057803226	2.9563706751847807
5	0.8101840720548444	1.9972195930291334
6	0.5479835308547061	1.62102931750485
7	0.37031282935634324	1.2780237101559155
8	0.2667982475991788	1.0523133314916058
9	0.1976605806166114	0.8770708736304521
>10	1.2921994532750627	12.195926655593732
>50	0.1341027434333586	4.631671241401193
>100	0.1079876548999688	10.70758625910983
>500	0.01178809750370851	3.8508204624516975
>1k	0.003841065478675913	2.863749574920257
>5k	2.6490106749489054E-4	0.8093191124361336
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7407	0.24113361119964216	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6649	0.2164570515548023	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5603	0.1824047014380444	No Hit
GTTCTAGACATGTATTTTCCAGCTGCCTCTAGTTTTTGAACTT	5128	0.16694115812498514	No Hit
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA	3648	0.1187600126442952	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.510965605498641E-5	0.0
2	0.0	0.0	0.0	6.510965605498641E-5	0.0
3	0.0	0.0	0.0	6.510965605498641E-5	0.0
4	0.0	0.0	0.0	6.510965605498641E-5	0.0
5	0.0	0.0	0.0	6.510965605498641E-5	0.0
6	0.0	0.0	0.0	6.510965605498641E-5	0.0
7	0.0	0.0	0.0	6.510965605498641E-5	0.0
8	0.0	0.0	0.0	6.510965605498641E-5	0.0
9	0.0	0.0	0.0	6.510965605498641E-5	0.0
10	0.0	0.0	0.0	1.3021931210997282E-4	0.0
11	0.0	0.0	0.0	2.2788379619245242E-4	0.0
12	0.0	0.0	0.0	2.2788379619245242E-4	0.0
13	0.0	0.0	0.0	2.6043862421994563E-4	0.0
14	0.0	0.0	0.0	2.6043862421994563E-4	0.0
15	0.0	0.0	0.0	3.581031083024252E-4	0.0
16	0.0	0.0	0.0	5.534320764673845E-4	0.0
17	0.0	0.0	0.0	7.487610446323436E-4	0.0
18	0.0	0.0	0.0	9.115351847698097E-4	0.0
19	0.0	0.0	0.0	0.001106864152934769	0.0
20	0.0	0.0	0.0	0.001269638293072235	0.0
21	0.0	0.0	0.0	0.0016277414013746601	0.0
22	0.0	0.0	0.0	0.002213728305869538	0.0
23	0.0	0.0	0.0	0.0029299345224743883	0.0
24	0.0	0.0	0.0	0.004557675923849048	0.0
25	0.0	0.0	0.0	0.005924978701003763	0.0
26	0.0	0.0	0.0	0.008106152178845808	0.0
27	0.0	0.0	0.0	0.020704870625485677	0.0
28	0.0	0.0	0.0	0.051143634831191824	0.0
29	0.0	0.0	0.0	0.09584141371293999	0.0
30	0.0	0.0	0.0	0.1638158946343458	0.0
31	0.0	0.0	0.0	0.3185164374209935	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGAC	1050	0.0	22.72857	3
GTTCTAG	1085	0.0	22.336405	1
GTCTAGA	715	0.0	20.699299	1
GGTATCA	2530	0.0	20.035574	1
AAGACGG	730	0.0	19.767122	5
CTAGAAC	755	0.0	19.60265	3
GACGGAC	705	0.0	18.893618	7
CTCTAGT	1165	0.0	18.5794	27
CTAGACA	1470	0.0	18.2483	4
CTAGCGG	510	0.0	18.137255	29
ACGGACC	745	0.0	18.127516	8
CCTCTAG	1745	0.0	17.704872	26
GACATGT	1265	0.0	17.695652	7
TAGAACT	975	0.0	17.456411	4
TCTAGCG	530	0.0	17.452831	28
ACTGATC	935	0.0	17.411764	8
TATACTG	500	0.0	17.02	5
CAAGACG	845	0.0	16.857986	4
GCGCAAG	830	0.0	16.716866	1
CTAGTTT	1310	0.0	16.522902	29
>>END_MODULE