FastQCFastQC Report
Fri 10 Feb 2017
ERR1630179.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630179.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1562871
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT351762.250729586766918No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT301621.929909762226057No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT237461.5193832376440537No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126260.8078721788298586No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA45360.2902350865810422No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT37650.2409028000391587No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36450.23322462314548034No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30210.19329810329835284No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29400.18811533389511995No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA26510.16962372454284455No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA26120.16712831705239908No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18710.11971557473393517No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17340.11094965611365237No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCA551.9032266E-523.54545413
CATAGCG400.001931496223.1250024
ACCGTCC400.001931496223.1250028
CGACGAG502.7027048E-422.224
CAATGCG502.7027048E-422.219
GTACTAG705.1038605E-621.1428591
GCGTTAT1550.020.2903231
AGCCGTC656.9041605E-519.92307712
CGTTATT801.6172768E-518.52
TGCGACG609.2388416E-418.522
ACGAGTC609.2388416E-418.526
CTTATAC14300.018.3706337
AACAGTG13750.017.4909082
AAGCCGT752.0678659E-417.26666611
GAACAGT14150.017.257951
TTTAGCG1005.880511E-616.65000226
CAGTCGG1901.8189894E-1216.55263110
CCGGTTA1251.6587182E-716.27999923
CCGTCAG803.3835392E-416.187514
CGGTTAT1302.5904956E-715.65384624
Produced by FastQC (version 0.11.5)