##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630179.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1562871
Sequences flagged as poor quality	0
Sequence length	43
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.636708339971758	31.0	31.0	34.0	30.0	34.0
2	31.77714027581291	31.0	31.0	34.0	30.0	34.0
3	31.864230637077533	33.0	31.0	34.0	30.0	34.0
4	35.58143506405839	37.0	35.0	37.0	33.0	37.0
5	35.533248745417886	37.0	35.0	37.0	33.0	37.0
6	35.64053719084941	37.0	35.0	37.0	33.0	37.0
7	35.608132724965785	37.0	35.0	37.0	33.0	37.0
8	35.63617854576609	37.0	35.0	37.0	33.0	37.0
9	37.26228396329576	39.0	37.0	39.0	34.0	39.0
10	37.16833954945738	39.0	37.0	39.0	33.0	39.0
11	37.286356327553584	39.0	37.0	39.0	34.0	39.0
12	37.17164692415433	39.0	37.0	39.0	33.0	39.0
13	37.23775218812045	39.0	37.0	39.0	34.0	39.0
14	38.264531109733305	40.0	38.0	41.0	33.0	41.0
15	38.24327663639546	40.0	38.0	41.0	33.0	41.0
16	38.18803791227811	40.0	37.0	41.0	33.0	41.0
17	38.28274374532511	40.0	38.0	41.0	34.0	41.0
18	38.340289121750935	40.0	38.0	41.0	34.0	41.0
19	38.39647802025887	40.0	38.0	41.0	34.0	41.0
20	38.39078465209221	40.0	38.0	41.0	34.0	41.0
21	38.36172915103038	40.0	38.0	41.0	34.0	41.0
22	38.31028088690621	40.0	38.0	41.0	34.0	41.0
23	38.23955016120972	40.0	38.0	41.0	34.0	41.0
24	38.212377093182994	40.0	38.0	41.0	34.0	41.0
25	38.16734970448616	40.0	37.0	41.0	33.0	41.0
26	37.951406737984136	40.0	37.0	41.0	33.0	41.0
27	37.78750325522708	40.0	37.0	41.0	33.0	41.0
28	37.67529053901442	40.0	36.0	41.0	33.0	41.0
29	37.542278921292926	39.0	36.0	41.0	33.0	41.0
30	37.367337419403135	39.0	36.0	41.0	33.0	41.0
31	37.11153063816528	39.0	35.0	41.0	32.0	41.0
32	36.88327955410267	39.0	35.0	40.0	31.0	41.0
33	36.72201480480475	39.0	35.0	40.0	31.0	41.0
34	36.569390563904506	39.0	35.0	40.0	31.0	41.0
35	36.40524969751182	38.0	35.0	40.0	30.0	41.0
36	36.19213421965089	38.0	35.0	40.0	30.0	41.0
37	36.02457080590784	38.0	35.0	40.0	30.0	41.0
38	35.81554459709087	38.0	35.0	40.0	29.0	41.0
39	35.58135316350486	38.0	35.0	40.0	27.0	41.0
40	35.332191844368474	38.0	35.0	40.0	26.0	41.0
41	35.030528431329266	38.0	34.0	40.0	24.0	41.0
42	34.78486964055255	38.0	34.0	40.0	23.0	41.0
43	33.70474530527471	37.0	33.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	8.0
10	12.0
11	29.0
12	23.0
13	7.0
14	6.0
15	11.0
16	16.0
17	32.0
18	58.0
19	164.0
20	308.0
21	653.0
22	1239.0
23	2078.0
24	3593.0
25	5665.0
26	8855.0
27	12940.0
28	18222.0
29	25120.0
30	32509.0
31	41436.0
32	52366.0
33	66809.0
34	86177.0
35	112330.0
36	157287.0
37	249477.0
38	338788.0
39	346649.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.67732781528354	20.543346187881152	14.337651667987952	20.44167432884736
2	18.223960902723256	24.69359275333665	34.38505161334493	22.697394730595168
3	20.790903407894827	26.418047298849363	30.871453881990263	21.91959541126555
4	13.855910052717082	17.023030051744513	34.356834313260656	34.76422558227774
5	12.476461588960317	40.64686080936942	32.56058881379205	14.316088787878206
6	30.477243483307326	35.71555169940449	16.919630602909645	16.887574214378535
7	26.049942701604934	33.82646424432983	21.598391677880006	18.525201376185237
8	29.206313252981214	32.585414919081614	20.163148462029177	18.045123365908
9	27.40021409316572	13.154060699827433	22.350149180578562	37.09557602642828
10	18.974630663695212	29.55938142047552	30.730047457531683	20.735940458297584
11	31.41314926183927	24.90237518003725	20.398484583820416	23.28599097430306
12	20.232635962916966	31.42178721084466	28.60415223009449	19.74142459614389
13	30.972421908142127	21.031358314281857	26.348303858731782	21.64791591884423
14	22.505376323445763	23.721599543404416	29.0717532029195	24.701270930230326
15	25.62214027901215	28.40138437529393	25.686636964919053	20.289838380774867
16	20.749569222283863	27.73696613476096	28.314876915625153	23.198587727330022
17	19.16709696449675	29.2471355601326	29.291668986115937	22.29409848925471
18	18.71689985929741	26.013471361359958	32.18627769022523	23.0833510891174
19	19.732978601560845	27.365342373106927	34.06672719629451	18.834951829037713
20	20.987784660410234	26.495788839897855	33.86024822266201	18.656178277029902
21	21.994905529631044	26.543969399905688	32.03277813715911	19.428346933304155
22	21.560896580715873	26.878162049203034	32.54043359944615	19.02050777063494
23	20.69204688038872	27.524664543650758	31.96431439319048	19.818974182770045
24	19.419005151416847	28.46626497004551	32.4687066302977	19.64602324823994
25	19.740592793647078	28.375406543470316	32.42423718912181	19.45976347376079
26	19.708152496271286	28.282436618249363	32.2027217857392	19.806689099740158
27	18.971943301782424	28.799433862423708	32.4866863611904	19.74193647460347
28	18.965288881807904	28.83712091401018	33.09486195597717	19.102728248204745
29	18.929457389637406	28.96240316699203	33.2064514601653	18.901687983205267
30	18.988707321333624	30.16442175969738	32.32723622103168	18.51963469793732
31	19.632266514638765	29.891206631897322	31.78931594482206	18.687210908641852
32	18.07468434694866	30.016488884879173	32.40990459225361	19.49892217591855
33	18.219034072549814	29.63795476402083	32.85741433554017	19.285596827889186
34	18.682220093660963	28.715741734282613	32.83796295407618	19.764075217980242
35	18.458977100477263	29.476649064446136	32.54683208019088	19.51754175488572
36	18.421929896965263	30.100244997827712	31.517380513170952	19.960444592036065
37	18.584579277496353	29.433011425767063	32.159787979942045	19.82262131679454
38	18.537614428830018	29.44919958205124	32.19849878844767	19.81468720067107
39	17.896166734170638	29.00789636508707	33.0298533916107	20.06608350913159
40	17.736332685167234	28.755092390862714	33.62376037433672	19.884814549633333
41	17.447889173194717	28.760531099495733	33.51255477899328	20.27902494831627
42	17.079144727875814	28.980382897884727	33.412290585723326	20.528181788516132
43	17.03704272457548	28.510670426413952	33.38893613100505	21.063350718005516
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2056.0
1	1493.0
2	930.0
3	2317.5
4	3705.0
5	3705.0
6	5465.0
7	7225.0
8	7004.5
9	6784.0
10	9639.0
11	12494.0
12	12494.0
13	23487.5
14	34481.0
15	52750.0
16	71019.0
17	60846.5
18	50674.0
19	50674.0
20	55447.0
21	60220.0
22	44068.5
23	27917.0
24	29316.0
25	30715.0
26	30715.0
27	32132.5
28	33550.0
29	36733.0
30	39916.0
31	44119.0
32	48322.0
33	48322.0
34	54405.5
35	60489.0
36	65554.0
37	70619.0
38	74266.5
39	77914.0
40	77914.0
41	84029.0
42	90144.0
43	93658.5
44	97173.0
45	100975.0
46	104777.0
47	104777.0
48	107640.0
49	110503.0
50	104528.5
51	98554.0
52	95353.5
53	92153.0
54	92153.0
55	89684.0
56	87215.0
57	81100.5
58	74986.0
59	67749.0
60	60512.0
61	60512.0
62	51643.0
63	42774.0
64	34822.0
65	26870.0
66	21463.5
67	16057.0
68	16057.0
69	12611.5
70	9166.0
71	7198.0
72	5230.0
73	4169.5
74	3109.0
75	3109.0
76	2573.0
77	2037.0
78	1738.0
79	1439.0
80	1006.5
81	574.0
82	574.0
83	414.5
84	255.0
85	198.5
86	142.0
87	117.5
88	93.0
89	93.0
90	75.5
91	58.0
92	34.5
93	11.0
94	9.0
95	7.0
96	7.0
97	4.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1562871.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.25977047217361
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.4300378564882	33.82775932707707
2	12.847773420472851	11.372790053372423
3	4.389178881070275	5.827921495124463
4	1.9166886866126907	3.393288053443583
5	1.006455514480045	2.2272745030670094
6	0.5987443143646344	1.5900171555178606
7	0.3900600848045665	1.208477887666468
8	0.29349318795187335	1.0391952907117137
9	0.21942315745187324	0.8740456726589568
>10	1.5944206828429262	14.361404732846328
>50	0.19674444443353267	6.023111943243917
>100	0.10975882795973477	8.217572242900841
>500	0.004043726997367102	1.1253017208008331
>1k	0.0025995387840217084	2.410593797021404
>5k	0.0	0.0
>10k+	5.776752853381574E-4	6.501246124547159
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	35176	2.250729586766918	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	30162	1.929909762226057	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	23746	1.5193832376440537	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12626	0.8078721788298586	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	4536	0.2902350865810422	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3765	0.2409028000391587	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3645	0.23322462314548034	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3021	0.19329810329835284	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2940	0.18811533389511995	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2651	0.16962372454284455	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	2612	0.16712831705239908	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1871	0.11971557473393517	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1734	0.11094965611365237	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	6.39848074473197E-5	0.0
9	0.0	0.0	0.0	6.39848074473197E-5	0.0
10	0.0	0.0	0.0	6.39848074473197E-5	0.0
11	0.0	0.0	0.0	1.9195442234195913E-4	0.0
12	0.0	0.0	0.0	5.758632670258773E-4	0.0
13	0.0	0.0	0.0	0.001087741726604435	0.0
14	0.0	0.0	0.0	0.001983529030866911	0.0
15	0.0	0.0	0.0	0.003071270757471346	0.0
16	0.0	0.0	0.0	0.006142541514942692	0.0
17	0.0	0.0	0.0	0.010429523613913113	0.0
18	0.0	0.0	0.0	0.011645234955412188	0.0
19	0.0	0.0	0.0	0.013500794371384458	0.0
20	0.0	0.0	0.0	0.016060186669277245	0.0
21	0.0	0.0	0.0	0.02213874337677262	0.0
22	0.0	6.39848074473197E-5	0.0	0.031864434108765216	0.0
23	0.0	6.39848074473197E-5	0.0	0.04530124367270235	0.0
24	0.0	6.39848074473197E-5	0.0	0.06366488341008311	0.0
25	0.0	6.39848074473197E-5	0.0	0.07076719703673559	0.0
26	0.0	6.39848074473197E-5	0.0	0.08202852314746387	0.0
27	0.0	6.39848074473197E-5	0.0	0.10307952479763205	0.0
28	0.0	6.39848074473197E-5	0.0	0.15695473266827525	0.0
29	0.0	6.39848074473197E-5	0.0	0.24403805560407738	0.0
30	0.0	6.39848074473197E-5	0.0	0.4043839830670606	0.0
31	0.0	6.39848074473197E-5	0.0	0.8150384772639584	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGTCA	55	1.9032266E-5	23.545454	13
CATAGCG	40	0.0019314962	23.125002	4
ACCGTCC	40	0.0019314962	23.125002	8
CGACGAG	50	2.7027048E-4	22.2	24
CAATGCG	50	2.7027048E-4	22.2	19
GTACTAG	70	5.1038605E-6	21.142859	1
GCGTTAT	155	0.0	20.290323	1
AGCCGTC	65	6.9041605E-5	19.923077	12
CGTTATT	80	1.6172768E-5	18.5	2
TGCGACG	60	9.2388416E-4	18.5	22
ACGAGTC	60	9.2388416E-4	18.5	26
CTTATAC	1430	0.0	18.37063	37
AACAGTG	1375	0.0	17.490908	2
AAGCCGT	75	2.0678659E-4	17.266666	11
GAACAGT	1415	0.0	17.25795	1
TTTAGCG	100	5.880511E-6	16.650002	26
CAGTCGG	190	1.8189894E-12	16.552631	10
CCGGTTA	125	1.6587182E-7	16.279999	23
CCGTCAG	80	3.3835392E-4	16.1875	14
CGGTTAT	130	2.5904956E-7	15.653846	24
>>END_MODULE