FastQCFastQC Report
Fri 10 Feb 2017
ERR1630178.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630178.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394521
Sequences flagged as poor quality0
Sequence length43
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT211965.372591066128293No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT135463.433530787968194No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT122693.1098471310779403No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57491.4572101358356082No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA34350.8706760856836517No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA24050.6095999959444491No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA22560.5718326781083897No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16930.42912798051307793No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13130.33280864643453706No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA12120.3072079813241881No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11720.2970691040527627No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10790.27349621439669874No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA9910.25119068439956305No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA8370.21215600690457542No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8370.21215600690457542No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA8200.20784698406421964No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8200.20784698406421964No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA7830.19846852258815123No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7630.19339908395243854No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7110.18021854349958558No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA6760.1713470258870884No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG6240.15816648543423545No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA6200.1571525977070929No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA5770.14625330464031067No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA5730.1452394169131681No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA5240.13281929225567207No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA4870.12344083077960363No Hit
GTACATGGGAAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA4820.12217347112067545No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4810.12191999918888981No Hit
GTACATGGGAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAA4780.12115958339353292No Hit
GTACATGGGAACATGGTATCAACGCAAAAAAAAAAAAAAAAAA4760.12065263952996165No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4710.11938527987103349No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4680.11862486407567657No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA4610.11685056055317715No Hit
GTACATGGGAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA4560.11558320089424898No Hit
GAACATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA4490.11380889737174953No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA4430.11228806578103573No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT4390.11127417805389321No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4380.11102070612210757No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC4370.11076723419032193No Hit
GAACTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA4230.10721862714532307No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4150.105190851691038No Hit
GAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAA4100.10392349203210982No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4050.10265613237318166No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA4040.10240266044139602No Hit
ACGCAGAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAA3960.10037488498711095No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGAGC200.001840471437.08
GTTGTGC251.2321562E-437.018
GTTCCGA200.001840471437.033
GGTTGTG251.2321562E-437.017
GTTATAC200.001840471437.03
ATACGGG507.3068804E-933.3000033
TCGCAGG303.5952317E-430.83333412
GCCGGAA303.5952317E-430.83333415
AGGTTGT303.5952317E-430.83333416
AGTAATG303.5952317E-430.8333345
TACGGGC502.7131318E-729.64
GCGTTAT250.005492022329.61
GTAGCGA250.005492022329.69
CGGGCGT502.7131318E-729.66
TAAGGTA250.005492022329.65
TGTCGGG250.005492022329.623
TGACTCG405.930395E-527.7514
TATTGAC556.244245E-726.9090930
CGTGGCT358.85903E-426.4285719
CGTGACT358.85903E-426.4285712
Produced by FastQC (version 0.11.5)