##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630178.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	394521
Sequences flagged as poor quality	0
Sequence length	43
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.657721642193952	31.0	31.0	34.0	30.0	34.0
2	31.7112295669939	31.0	31.0	34.0	30.0	34.0
3	31.733000271214966	33.0	31.0	34.0	30.0	34.0
4	35.49576575137952	37.0	35.0	37.0	33.0	37.0
5	35.49288631023444	37.0	35.0	37.0	33.0	37.0
6	35.63144420702574	37.0	35.0	37.0	33.0	37.0
7	35.575992152508995	37.0	35.0	37.0	33.0	37.0
8	35.65412487548191	37.0	35.0	37.0	33.0	37.0
9	37.22235318272031	39.0	37.0	39.0	34.0	39.0
10	37.115220736031795	39.0	37.0	39.0	33.0	39.0
11	37.251287003733644	39.0	37.0	39.0	34.0	39.0
12	37.14662844309935	39.0	37.0	39.0	33.0	39.0
13	37.23854243500346	39.0	37.0	39.0	34.0	39.0
14	38.085067715026575	40.0	37.0	41.0	33.0	41.0
15	38.0289718418031	40.0	37.0	41.0	33.0	41.0
16	37.971646629710456	40.0	37.0	41.0	33.0	41.0
17	38.19509988061472	40.0	37.0	41.0	33.0	41.0
18	38.35863236684486	40.0	38.0	41.0	34.0	41.0
19	38.445248795374646	40.0	38.0	41.0	34.0	41.0
20	38.51547319407585	40.0	38.0	41.0	34.0	41.0
21	38.50722521741555	40.0	38.0	41.0	34.0	41.0
22	38.46404627383587	40.0	38.0	41.0	34.0	41.0
23	38.377249880234515	40.0	38.0	41.0	34.0	41.0
24	38.32355945564368	40.0	38.0	41.0	34.0	41.0
25	38.26843184519962	40.0	37.0	41.0	34.0	41.0
26	37.98493616309398	40.0	37.0	41.0	33.0	41.0
27	37.76266916083048	40.0	36.0	41.0	33.0	41.0
28	37.58008572420733	39.0	36.0	41.0	33.0	41.0
29	37.33727228715328	39.0	35.0	41.0	33.0	41.0
30	36.99345028528266	38.0	35.0	40.0	32.0	41.0
31	36.548057010906895	38.0	35.0	40.0	32.0	41.0
32	36.20177886601727	37.0	35.0	40.0	31.0	41.0
33	35.93073119048162	37.0	35.0	40.0	31.0	41.0
34	35.68473414596435	37.0	35.0	40.0	30.0	41.0
35	35.42691517054859	37.0	35.0	40.0	30.0	41.0
36	35.07502515708923	36.0	35.0	40.0	27.0	41.0
37	34.76958894456822	36.0	34.0	40.0	25.0	41.0
38	34.40856887212595	35.0	33.0	40.0	24.0	41.0
39	34.007231554213845	35.0	33.0	40.0	22.0	41.0
40	33.53659501015155	35.0	33.0	40.0	20.0	41.0
41	33.0078931159558	35.0	33.0	40.0	15.0	41.0
42	32.49517769649778	35.0	33.0	40.0	12.0	41.0
43	31.39964666012709	35.0	31.0	39.0	9.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	4.0
11	15.0
12	12.0
13	5.0
14	9.0
15	2.0
16	3.0
17	8.0
18	15.0
19	47.0
20	89.0
21	180.0
22	327.0
23	681.0
24	1201.0
25	1862.0
26	2861.0
27	4220.0
28	5945.0
29	7834.0
30	9979.0
31	12104.0
32	14966.0
33	19111.0
34	24786.0
35	31434.0
36	43977.0
37	75553.0
38	73474.0
39	63812.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.88276923154915	18.159743080849942	15.558107173002197	16.399380514598715
2	16.551463673670096	30.064305829094017	32.8945734194124	20.489657077823487
3	21.063771003317946	31.160064990203313	32.142015253940855	15.634148752537888
4	12.39756565556713	18.00208353927928	31.49439446822856	38.105956336925026
5	11.401674435581375	45.53876726460695	28.76627606641978	14.293282233391885
6	27.375222104780228	34.68839428065933	19.403783322053833	18.53260029250661
7	23.099911031351944	36.427211732708784	21.10888900717579	19.36398822876349
8	34.66355403134434	29.822747078102307	19.369311139330986	16.144387751222368
9	30.07672595375151	10.94162287939045	21.61050996017956	37.37114120667847
10	20.40753217192494	33.522169922513626	24.527464951168632	21.542832954392797
11	30.249847283161095	29.10668887080789	16.998841633271738	23.64462221275927
12	20.123897080256818	35.30534496262556	27.50956222862661	17.061195728491004
13	33.68718015010608	20.988489839577614	26.22978244504095	19.09454756527536
14	23.431705790059336	24.344458216419405	31.80033508989382	20.42350090362744
15	26.905031671317875	28.77971007880442	27.192975785826356	17.122282464051345
16	17.107327620075992	28.470220850094165	30.576572603232783	23.845878926597063
17	16.9907305314546	31.164374013043666	31.563845777537825	20.28104967796391
18	15.536055114936847	24.948228357932784	36.91894728037291	22.59676924675746
19	16.109155152704165	29.52365019859526	39.02479208964795	15.342402559052623
20	17.881684371681104	26.731656869976504	39.99204098134193	15.394617777000462
21	19.77359887052907	26.610243814651184	37.64590478073411	15.970252534085638
22	19.66967537849696	28.03450259935466	35.459709369083015	16.836112653065364
23	17.54735489365585	29.55660154972739	36.127861381269945	16.768182175346812
24	15.927922721477437	30.97021451329587	36.924270190940405	16.177592574286287
25	15.960367128745997	30.81052719627092	36.75723218789367	16.471873487089407
26	17.42619531026232	30.419673477457476	36.54735742837517	15.606773783905039
27	15.718554905822504	31.477918792662496	36.93415559628004	15.869370705234957
28	14.834951751617783	32.97188235860702	37.3706342628149	14.82253162696029
29	15.630346673561101	32.82943113294349	36.78410021266295	14.756121980832452
30	14.574129133810368	34.70512342815718	37.45504041609952	13.265707021932926
31	15.20324646850231	33.426611004230445	36.017094147079625	15.35304838018762
32	13.091825276727981	34.432641101487626	36.64367676245371	15.831856859330681
33	14.1130636898923	33.82177374588425	37.773401162422275	14.29176140180117
34	13.612456624615673	34.33733565513623	36.0969378055921	15.953269914656001
35	13.899133379465226	35.00447377959602	36.20745156785063	14.888941273088124
36	13.357970805102898	35.02196334288922	35.68682021996295	15.933245632044935
37	13.78101545925312	35.59455643679297	35.249074193769154	15.375353910184755
38	13.736404399258847	34.414644594330845	35.34083103307555	16.508119973334754
39	13.879362568785947	33.58933998443682	36.89664175037578	15.63465569640146
40	13.407904775664667	34.66152625589005	36.11163917763566	15.818929790809616
41	13.12807176297333	34.25875935628268	36.37448957089737	16.238679309846624
42	12.718714593139529	34.45241191216691	36.15371551831208	16.675157976381485
43	12.757495798702731	34.9253905368789	35.63587236167403	16.68124130274434
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	24.0
1	80.0
2	136.0
3	851.5
4	1567.0
5	1567.0
6	2327.0
7	3087.0
8	2964.5
9	2842.0
10	3916.0
11	4990.0
12	4990.0
13	10064.0
14	15138.0
15	27090.5
16	39043.0
17	31859.5
18	24676.0
19	24676.0
20	26085.5
21	27495.0
22	18587.0
23	9679.0
24	10240.0
25	10801.0
26	10801.0
27	10659.5
28	10518.0
29	10017.0
30	9516.0
31	9392.0
32	9268.0
33	9268.0
34	9607.5
35	9947.0
36	10031.5
37	10116.0
38	11005.5
39	11895.0
40	11895.0
41	13811.0
42	15727.0
43	17873.5
44	20020.0
45	23317.5
46	26615.0
47	26615.0
48	30257.0
49	33899.0
50	29198.0
51	24497.0
52	21664.5
53	18832.0
54	18832.0
55	17625.5
56	16419.0
57	14941.0
58	13463.0
59	11333.0
60	9203.0
61	9203.0
62	7480.5
63	5758.0
64	4629.5
65	3501.0
66	2962.5
67	2424.0
68	2424.0
69	2084.0
70	1744.0
71	1301.0
72	858.0
73	657.0
74	456.0
75	456.0
76	331.0
77	206.0
78	149.0
79	92.0
80	71.0
81	50.0
82	50.0
83	32.0
84	14.0
85	9.0
86	4.0
87	2.5
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	394521.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.44555347937234
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.85897743591455	18.284914533185738
2	11.642996344039759	5.925249722648006
3	4.799029588363235	3.6634189211936072
4	2.496999517477995	2.5415013903981296
5	1.6129994971399824	2.0521832483338054
6	1.1399996420821017	1.7404753115439269
7	0.7989997491106274	1.4231693592199688
8	0.5809998175631021	1.182708954344599
9	0.45399985744172916	1.0397049886944842
>10	3.8449987926507667	19.874497759904884
>50	0.43899986215180403	7.6023656258504895
>100	0.30499990422847434	14.954347084085859
>500	0.01399999560392997	2.5842495998974986
>1k	0.007999997487959981	3.706143700522555
>5k	9.999996859949977E-4	1.4628644101822292
>10k+	0.0029999990579849933	11.962205389994221
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	21196	5.372591066128293	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13546	3.433530787968194	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12269	3.1098471310779403	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5749	1.4572101358356082	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	3435	0.8706760856836517	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	2405	0.6095999959444491	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2256	0.5718326781083897	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1693	0.42912798051307793	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1313	0.33280864643453706	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	1212	0.3072079813241881	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1172	0.2970691040527627	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1079	0.27349621439669874	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	991	0.25119068439956305	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	837	0.21215600690457542	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	837	0.21215600690457542	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	820	0.20784698406421964	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	820	0.20784698406421964	No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA	783	0.19846852258815123	No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	763	0.19339908395243854	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	711	0.18021854349958558	No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA	676	0.1713470258870884	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	624	0.15816648543423545	No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	620	0.1571525977070929	No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA	577	0.14625330464031067	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	573	0.1452394169131681	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	524	0.13281929225567207	No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	487	0.12344083077960363	No Hit
GTACATGGGAAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	482	0.12217347112067545	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	481	0.12191999918888981	No Hit
GTACATGGGAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	478	0.12115958339353292	No Hit
GTACATGGGAACATGGTATCAACGCAAAAAAAAAAAAAAAAAA	476	0.12065263952996165	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	471	0.11938527987103349	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	468	0.11862486407567657	No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA	461	0.11685056055317715	No Hit
GTACATGGGAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	456	0.11558320089424898	No Hit
GAACATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	449	0.11380889737174953	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	443	0.11228806578103573	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT	439	0.11127417805389321	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	438	0.11102070612210757	No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC	437	0.11076723419032193	No Hit
GAACTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	423	0.10721862714532307	No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	415	0.105190851691038	No Hit
GAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAA	410	0.10392349203210982	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	405	0.10265613237318166	No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	404	0.10240266044139602	No Hit
ACGCAGAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAA	396	0.10037488498711095	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.5347193178563374E-4	0.0
5	0.0	0.0	0.0	2.5347193178563374E-4	0.0
6	0.0	0.0	0.0	2.5347193178563374E-4	0.0
7	0.0	0.0	0.0	7.604157953569012E-4	0.0
8	0.0	0.0	0.0	7.604157953569012E-4	0.0
9	0.0	0.0	0.0	0.0015208315907138024	0.0
10	0.0	0.0	0.0	0.001774303522499436	0.0
11	0.0	0.0	0.0	0.00202777545428507	0.0
12	0.0	0.0	0.0	0.00202777545428507	0.0
13	0.0	0.0	0.0	0.002534719317856337	0.0
14	0.0	0.0	0.0	0.0030416631814276047	0.0
15	0.0	0.0	0.0	0.003548607044998872	0.0
16	0.0	0.0	0.0	0.007857629885354645	0.0
17	0.0	0.0	0.0	0.011152764998567884	0.0
18	0.0	0.0	0.0	0.013180540452852954	0.0
19	0.0	0.0	0.0	0.015968731702494923	0.0
20	0.0	0.0	0.0	0.020277754542850697	0.0
21	0.0	0.0	0.0	0.029149272155347877	0.0
22	0.0	0.0	0.0	0.042583284539986464	0.0
23	0.0	0.0	0.0	0.05905896010605265	0.0
24	0.0	0.0	0.0	0.08263184976211659	0.0
25	0.0	0.0	0.0	0.09302419896532757	0.0
26	0.0	0.0	0.0	0.10392349203210982	0.0
27	0.0	0.0	0.0	0.11963875180281912	0.0
28	0.0	0.0	0.0	0.15284357486673714	0.0
29	0.0	0.0	0.0	0.2195066929263588	0.0
30	0.0	0.0	0.0	0.3916141346088041	0.0
31	0.0	0.0	0.0	0.8856309296590043	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGAGC	20	0.0018404714	37.0	8
GTTGTGC	25	1.2321562E-4	37.0	18
GTTCCGA	20	0.0018404714	37.0	33
GGTTGTG	25	1.2321562E-4	37.0	17
GTTATAC	20	0.0018404714	37.0	3
ATACGGG	50	7.3068804E-9	33.300003	3
TCGCAGG	30	3.5952317E-4	30.833334	12
GCCGGAA	30	3.5952317E-4	30.833334	15
AGGTTGT	30	3.5952317E-4	30.833334	16
AGTAATG	30	3.5952317E-4	30.833334	5
TACGGGC	50	2.7131318E-7	29.6	4
GCGTTAT	25	0.0054920223	29.6	1
GTAGCGA	25	0.0054920223	29.6	9
CGGGCGT	50	2.7131318E-7	29.6	6
TAAGGTA	25	0.0054920223	29.6	5
TGTCGGG	25	0.0054920223	29.6	23
TGACTCG	40	5.930395E-5	27.75	14
TATTGAC	55	6.244245E-7	26.90909	30
CGTGGCT	35	8.85903E-4	26.42857	19
CGTGACT	35	8.85903E-4	26.42857	12
>>END_MODULE