FastQCFastQC Report
Fri 10 Feb 2017
ERR1630177.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630177.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1264629
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG44900.3550448392374364No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT32120.2539875331025937No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC29030.22955348960050737No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG28680.22678587949509305No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA26650.21073374088369No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG25530.2018773885463642No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA23990.1896999040825412No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT23760.18788118887041177No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA23280.18408560929727216No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC23000.18187152121294073No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC21950.17356869089669777No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT21450.1696149621746773No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC19390.15332559983995306No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG19170.15158595920226406No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG18280.14454832207706766No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA18160.14359942718378274No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG17800.14075274250392802No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC17300.13679901378190756No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA17240.1363245663352651No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA17090.135138447718659No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG16990.1343477019742549No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC16810.13292435963432755No Hit
GAGTTTGACCAGCCACTCTCCAGCTCGGCTTTCGCGGCGCCGA16240.12841710889122424No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG16090.1272309902746181No Hit
GTTCGGAGCCATCGCTGCTGCCTGCTGATCCGCGCCTAGAGTT16030.12675654282797563No Hit
ATGCTGTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCA15990.126440244530214No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC14980.11845371251173269No Hit
CTCCAGCTCGGCTTTCGCGGCGCCGAGATGCTGTCCTGCCGCC14960.11829556336285187No Hit
CGGCAGGACAGCATCTCGGCGCCGCGAAAGCCGAGCTGGAGAG14940.11813741421397106No Hit
AGTCTGGGGTCCGAGGGAGCGCCGGTGACACAGCCCAGGGCCA14710.11631869900184165No Hit
CCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGGCTGCTGCC13890.1098345838977281No Hit
GCCAGGGACTTCTGCAGAAACTGACGGAGTCTGGGGTCCGAGG13760.10880661443000278No Hit
GTCCCTGGCTGCTGCCGCGGGGAAGCAGGAACTGGCCAAGTAC13590.10746234666451583No Hit
GTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCATCGTC13550.1071460483667542No Hit
TAGCAGATCTCTGCAGCTCAAGCCTCATTTCATCCTGCTCAGC13500.10675067549455217No Hit
AGGGAAGAGAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTG12890.1019271264536872No Hit
GGACAGCATCTCGGCGCCGCGAAAGCCGAGCTGGAGAGTGGCT12840.10153175358148517No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG250.005496548529.61
TCTCGTA400.001931294223.1252
TAGACCG450.00382601620.5555575
GGTATCA3700.019.51
TCTTATA5900.019.12712137
CCTTTAG1005.8787246E-616.6500021
GTAAGAC1005.8787246E-616.6500023
CGCGCAA1452.9795046E-916.58620621
CGATGCG1452.9795046E-916.58620633
TGTGCGC1352.2197128E-816.44444510
TCTAGCG803.382902E-416.187528
CGAACGA700.00259268615.85714316
GGTACCT1455.34601E-815.3103458
GACGTTA855.364681E-415.23529315
TACCTTC855.364681E-415.2352937
CCGACGT855.364681E-415.23529313
CTAGACT750.004104709714.7999994
TCCAACG750.004104709714.79999931
TACACAG1904.5474735E-1014.6052645
CGACCGG1651.645094E-814.57575726
Produced by FastQC (version 0.11.5)