##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630177.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1264629
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.75333635398208	31.0	31.0	34.0	30.0	34.0
2	31.944390805524783	33.0	31.0	34.0	30.0	34.0
3	32.10939255702661	33.0	31.0	34.0	30.0	34.0
4	35.77149187627359	37.0	35.0	37.0	35.0	37.0
5	35.68090720677764	37.0	35.0	37.0	33.0	37.0
6	35.74482713902655	37.0	35.0	37.0	35.0	37.0
7	35.70209523899895	37.0	35.0	37.0	33.0	37.0
8	35.68049364675332	37.0	35.0	37.0	33.0	37.0
9	37.413983864042336	39.0	37.0	39.0	34.0	39.0
10	37.29758846270329	39.0	37.0	39.0	34.0	39.0
11	37.39569312422853	39.0	37.0	39.0	34.0	39.0
12	37.218329644504436	39.0	37.0	39.0	34.0	39.0
13	37.28469851632376	39.0	37.0	39.0	34.0	39.0
14	38.49855649364359	40.0	38.0	41.0	34.0	41.0
15	38.49084435039842	40.0	38.0	41.0	34.0	41.0
16	38.4883171269993	40.0	38.0	41.0	34.0	41.0
17	38.403038361448296	40.0	38.0	41.0	34.0	41.0
18	38.36102920303109	40.0	38.0	41.0	34.0	41.0
19	38.361371595938415	40.0	38.0	41.0	34.0	41.0
20	38.307462504813664	40.0	38.0	41.0	34.0	41.0
21	38.26947428850675	40.0	38.0	41.0	34.0	41.0
22	38.24534705435349	40.0	38.0	41.0	34.0	41.0
23	38.20787203203469	40.0	38.0	41.0	33.0	41.0
24	38.19606066285053	40.0	38.0	41.0	33.0	41.0
25	38.15264002327955	40.0	38.0	41.0	33.0	41.0
26	37.9956121518643	40.0	37.0	41.0	33.0	41.0
27	37.88960635886098	40.0	37.0	41.0	33.0	41.0
28	37.81593415934634	40.0	37.0	41.0	33.0	41.0
29	37.74452744638941	40.0	37.0	41.0	32.0	41.0
30	37.69764887567816	40.0	37.0	41.0	32.0	41.0
31	37.55543799802155	40.0	37.0	41.0	32.0	41.0
32	37.4742007339702	40.0	37.0	41.0	32.0	41.0
33	37.429831990251685	39.0	37.0	41.0	31.0	41.0
34	37.29042351551325	39.0	36.0	41.0	31.0	41.0
35	37.23789822944121	39.0	36.0	41.0	31.0	41.0
36	37.12556884271988	39.0	36.0	41.0	31.0	41.0
37	37.039373602851114	39.0	36.0	41.0	31.0	41.0
38	36.97201867108852	39.0	36.0	41.0	31.0	41.0
39	36.83064519317523	39.0	35.0	40.0	30.0	41.0
40	36.73049882613794	39.0	35.0	40.0	30.0	41.0
41	36.604252314315104	39.0	35.0	40.0	30.0	41.0
42	36.55187568844301	39.0	35.0	40.0	30.0	41.0
43	35.29207380188182	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	0.0
12	1.0
13	0.0
14	0.0
15	3.0
16	8.0
17	18.0
18	47.0
19	77.0
20	183.0
21	384.0
22	725.0
23	1293.0
24	2273.0
25	3500.0
26	5261.0
27	7758.0
28	11306.0
29	15450.0
30	21191.0
31	27814.0
32	36087.0
33	46872.0
34	61247.0
35	82701.0
36	116187.0
37	173103.0
38	303133.0
39	348005.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.63198297682561	17.961789584138906	10.930715648621057	26.475511790414423
2	19.83815016103537	20.012825895974235	32.83990798882518	27.309115954165215
3	21.143750459620964	20.471458427728606	27.901305442149436	30.483485670500993
4	14.417667157719775	14.897807973721939	33.00019215121589	37.6843327173424
5	16.392238356071225	34.73722332794835	33.02691935737675	15.84361895860367
6	38.42502425612571	32.85493215796886	14.78623374918652	13.933809836718911
7	33.07665726469977	27.921232234908423	19.20413022317217	19.797980277219644
8	29.119212037680615	31.99444263890833	18.977107119953757	19.9092382034573
9	27.456431886347694	12.439379454369622	17.625169120746083	42.4790195385366
10	17.176183687073443	25.443430444818205	32.424292025566395	24.956093842541964
11	39.14697512076664	20.254477795464123	19.08045758874737	21.51808949502186
12	22.816335858184495	23.487204547736926	27.635773021178544	26.06068657290004
13	32.90791212284393	17.719900460925693	23.011570982477867	26.360616433752508
14	24.704557621247023	18.874389247755666	22.738526476935135	33.68252665406218
15	26.176372675306354	26.308031841749635	20.724259842214593	26.791335640729415
16	28.530739054695093	23.910411670141997	21.612109164031505	25.946740111131405
17	25.85422285903613	24.301119142452055	22.900945652835734	26.943712345676087
18	25.645149684215685	22.886633154862018	24.726698502090336	26.741518658831957
19	28.260699382981098	23.678327794159394	23.10179507191437	24.959177750945138
20	28.024108256255392	23.249269153245734	23.027227748217065	25.69939484228181
21	26.80169440998111	23.57292138643033	22.83420671200803	26.791177491580537
22	27.766325143579657	22.715357626624094	23.07024431671265	26.4480729130836
23	25.292477082211462	23.46909647019007	23.59300632833819	27.645420119260272
24	26.497652671257736	23.97169446533331	23.013310623115554	26.517342240293395
25	26.800666440513382	23.53053741453027	23.294420735251208	26.374375409705138
26	27.450580367839105	23.742536348605007	21.969842538799917	26.837040744755974
27	27.52135211196327	23.176125171888355	22.046861174304873	27.255661541843494
28	26.596574963882684	23.831574319424906	23.116107569888086	26.455743146804316
29	26.847162290284345	24.137513847934848	23.615147209181504	25.4001766525993
30	27.202128054947337	23.438257386158313	22.362764099194308	26.99685045970004
31	25.96714135133703	23.24855748207577	23.214950787938594	27.5693503786486
32	25.55690245914019	24.01803216595539	23.060280920333156	27.36478445457126
33	26.315939299193676	22.915495374532767	23.591662060572705	27.17690326570085
34	27.345964705854442	22.90632272389768	23.806744903050618	25.940967667197256
35	27.737462923908907	22.934552346972907	22.742401131082712	26.585583598035473
36	25.952591629639997	23.571972491537043	22.332557611758073	28.142878267064887
37	25.98596110005385	23.241361695801693	24.666997198387826	26.105680005756625
38	25.85311581499396	22.932338258888578	23.324469073538566	27.8900768525789
39	25.40587002195901	23.723321227015987	24.975862486152064	25.894946264872935
40	27.154841459431978	22.17108733075076	24.06263022593978	26.611440983877483
41	24.827597659076297	23.04391248342399	25.212611762026647	26.91587809547306
42	25.084985398879827	23.61182607705501	24.767342833352707	26.535845690712456
43	25.4624083426839	22.074379126210136	24.61520335212936	27.8480091789766
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	9.0
1	13.5
2	18.0
3	39.5
4	61.0
5	61.0
6	99.0
7	137.0
8	136.0
9	135.0
10	181.0
11	227.0
12	227.0
13	402.5
14	578.0
15	874.5
16	1171.0
17	1380.5
18	1590.0
19	1590.0
20	2186.5
21	2783.0
22	3584.5
23	4386.0
24	5684.0
25	6982.0
26	6982.0
27	8521.0
28	10060.0
29	12477.5
30	14895.0
31	18114.0
32	21333.0
33	21333.0
34	28024.5
35	34716.0
36	39337.5
37	43959.0
38	46041.0
39	48123.0
40	48123.0
41	51447.0
42	54771.0
43	60686.0
44	66601.0
45	69354.0
46	72107.0
47	72107.0
48	74566.0
49	77025.0
50	82429.5
51	87834.0
52	99085.5
53	110337.0
54	110337.0
55	109408.0
56	108479.0
57	100915.0
58	93351.0
59	94816.0
60	96281.0
61	96281.0
62	91065.5
63	85850.0
64	70932.0
65	56014.0
66	50769.5
67	45525.0
68	45525.0
69	41535.5
70	37546.0
71	38564.5
72	39583.0
73	34488.0
74	29393.0
75	29393.0
76	17564.0
77	5735.0
78	4442.5
79	3150.0
80	2500.5
81	1851.0
82	1851.0
83	1413.5
84	976.0
85	751.0
86	526.0
87	416.0
88	306.0
89	306.0
90	243.5
91	181.0
92	102.5
93	24.0
94	20.5
95	17.0
96	17.0
97	10.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1264629.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.55632649877288
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.46602334032777	50.03313300083639
2	7.0774056106670455	8.005441249622649
3	1.7920236096230582	3.0405081707805355
4	0.7228927906870475	1.6353664277482294
5	0.40549537237058186	1.1466664336766055
6	0.2595122614938116	0.8806236114887358
7	0.1722172604501371	0.6817982927519511
8	0.13557792753140852	0.6134231628394646
9	0.10323602298634753	0.5254785200205623
>10	0.684864985191568	7.637564218596128
>50	0.08161951421595769	3.235178559492495
>100	0.07526116594825398	8.824761223371262
>500	0.016147446637174184	6.399987771047816
>1k	0.0077226918699528704	7.340069357727189
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	4490	0.3550448392374364	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	3212	0.2539875331025937	No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC	2903	0.22955348960050737	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	2868	0.22678587949509305	No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA	2665	0.21073374088369	No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG	2553	0.2018773885463642	No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA	2399	0.1896999040825412	No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT	2376	0.18788118887041177	No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA	2328	0.18408560929727216	No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC	2300	0.18187152121294073	No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC	2195	0.17356869089669777	No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT	2145	0.1696149621746773	No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC	1939	0.15332559983995306	No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG	1917	0.15158595920226406	No Hit
GATCAGCAGGCAGCAGCGATGGCTCCGAACCTCGCTCCTAAAG	1828	0.14454832207706766	No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA	1816	0.14359942718378274	No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG	1780	0.14075274250392802	No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC	1730	0.13679901378190756	No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA	1724	0.1363245663352651	No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA	1709	0.135138447718659	No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG	1699	0.1343477019742549	No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC	1681	0.13292435963432755	No Hit
GAGTTTGACCAGCCACTCTCCAGCTCGGCTTTCGCGGCGCCGA	1624	0.12841710889122424	No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG	1609	0.1272309902746181	No Hit
GTTCGGAGCCATCGCTGCTGCCTGCTGATCCGCGCCTAGAGTT	1603	0.12675654282797563	No Hit
ATGCTGTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCA	1599	0.126440244530214	No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC	1498	0.11845371251173269	No Hit
CTCCAGCTCGGCTTTCGCGGCGCCGAGATGCTGTCCTGCCGCC	1496	0.11829556336285187	No Hit
CGGCAGGACAGCATCTCGGCGCCGCGAAAGCCGAGCTGGAGAG	1494	0.11813741421397106	No Hit
AGTCTGGGGTCCGAGGGAGCGCCGGTGACACAGCCCAGGGCCA	1471	0.11631869900184165	No Hit
CCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGGCTGCTGCC	1389	0.1098345838977281	No Hit
GCCAGGGACTTCTGCAGAAACTGACGGAGTCTGGGGTCCGAGG	1376	0.10880661443000278	No Hit
GTCCCTGGCTGCTGCCGCGGGGAAGCAGGAACTGGCCAAGTAC	1359	0.10746234666451583	No Hit
GTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCATCGTC	1355	0.1071460483667542	No Hit
TAGCAGATCTCTGCAGCTCAAGCCTCATTTCATCCTGCTCAGC	1350	0.10675067549455217	No Hit
AGGGAAGAGAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTG	1289	0.1019271264536872	No Hit
GGACAGCATCTCGGCGCCGCGAAAGCCGAGCTGGAGAGTGGCT	1284	0.10153175358148517	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	7.9074574440409E-5	0.0	0.0	0.0	0.0
6	7.9074574440409E-5	0.0	0.0	0.0	0.0
7	7.9074574440409E-5	0.0	0.0	0.0	0.0
8	7.9074574440409E-5	0.0	0.0	0.0	0.0
9	7.9074574440409E-5	0.0	0.0	0.0	0.0
10	7.9074574440409E-5	0.0	0.0	0.0	0.0
11	7.9074574440409E-5	0.0	0.0	0.0	0.0
12	7.9074574440409E-5	0.0	0.0	0.0	0.0
13	7.9074574440409E-5	0.0	0.0	0.0	0.0
14	7.9074574440409E-5	0.0	0.0	0.0	0.0
15	7.9074574440409E-5	0.0	0.0	0.0	0.0
16	7.9074574440409E-5	0.0	0.0	3.16298297761636E-4	0.0
17	7.9074574440409E-5	0.0	0.0	6.32596595523272E-4	0.0
18	7.9074574440409E-5	7.9074574440409E-5	0.0	9.488948932849081E-4	0.0
19	7.9074574440409E-5	7.9074574440409E-5	0.0	0.0011861186166061352	0.0
20	7.9074574440409E-5	7.9074574440409E-5	0.0	0.001344267765486953	0.0
21	7.9074574440409E-5	7.9074574440409E-5	0.0	0.0017396406376889982	0.0
22	7.9074574440409E-5	7.9074574440409E-5	0.0	0.0023722372332122704	0.0
23	7.9074574440409E-5	7.9074574440409E-5	0.0	0.00316298297761636	0.0
24	7.9074574440409E-5	7.9074574440409E-5	0.0	0.0041118778709012685	0.0
25	7.9074574440409E-5	7.9074574440409E-5	0.0	0.004823549040864949	0.0
26	7.9074574440409E-5	7.9074574440409E-5	0.0	0.006246891380792312	0.0
27	7.9074574440409E-5	7.9074574440409E-5	0.0	0.01122858957053808	0.0
28	7.9074574440409E-5	7.9074574440409E-5	0.0	0.025936460416454154	0.0
29	7.9074574440409E-5	1.58149148880818E-4	0.0	0.0491843853019344	0.0
30	1.58149148880818E-4	1.58149148880818E-4	0.0	0.08057699135477678	0.0
31	1.58149148880818E-4	1.58149148880818E-4	0.0	0.1931001107834788	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATACG	25	0.0054965485	29.6	1
TCTCGTA	40	0.0019312942	23.125	2
TAGACCG	45	0.003826016	20.555557	5
GGTATCA	370	0.0	19.5	1
TCTTATA	590	0.0	19.127121	37
CCTTTAG	100	5.8787246E-6	16.650002	1
GTAAGAC	100	5.8787246E-6	16.650002	3
CGCGCAA	145	2.9795046E-9	16.586206	21
CGATGCG	145	2.9795046E-9	16.586206	33
TGTGCGC	135	2.2197128E-8	16.444445	10
TCTAGCG	80	3.382902E-4	16.1875	28
CGAACGA	70	0.002592686	15.857143	16
GGTACCT	145	5.34601E-8	15.310345	8
GACGTTA	85	5.364681E-4	15.235293	15
TACCTTC	85	5.364681E-4	15.235293	7
CCGACGT	85	5.364681E-4	15.235293	13
CTAGACT	75	0.0041047097	14.799999	4
TCCAACG	75	0.0041047097	14.799999	31
TACACAG	190	4.5474735E-10	14.605264	5
CGACCGG	165	1.645094E-8	14.575757	26
>>END_MODULE