FastQCFastQC Report
Fri 10 Feb 2017
ERR1630176.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630176.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1745473
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC144200.826137098654634No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT70820.4057352935278862No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG51880.2972260241206825No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG51490.2949916727442934No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG50970.2920125375757746No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT49160.2816428555468919No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG45780.26227847695151973No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT43030.24652343519492997No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT40550.23231525208353265No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG38040.21793519578933618No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC35950.20596136405432794No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG35110.20114891493595147No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC34910.2000030937172904No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT34640.19845623507209792No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG34460.19742499597530297No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT33520.19203963624759593No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT32520.18631053015429055No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG31870.18258661119364206No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG30680.17576897494260868No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT29760.17049819733676774No Hit
GCACAGGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCC26690.15290984163032026No Hit
GCCCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGCTGGCCC26060.1493005047915379No Hit
GGTCCCCAGAGGGCCAGCAGCGCCAGCAGGGGCAGGAGGCGCA24720.1416235026265087No Hit
CACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACGAGGCT24510.14042039034691456No Hit
CTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCA24300.13921727806732043No Hit
CTAGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTG23770.13618085183786857No Hit
GCTCACACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACG23250.1332017166693498No Hit
TGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGA23020.13188402226788956No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22730.13022258150083102No Hit
TTGTGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCT22630.12964967089150048No Hit
GCCATGGCCCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGC22400.12833197649004024No Hit
GAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGA22130.1267851178448478No Hit
GTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGTTC19630.11246235261158438No Hit
CACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCTAGTGT19460.11148840457572247No Hit
CCACAGGGCCATGGCAGAAGGACAGTGATCTGCTTGATGGCCT19300.1105717476007936No Hit
TTCTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGC19210.11005612805239612No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19070.10925405319933336No Hit
GTTCCACAATGCCACGCTTCTGCAGGGACCCCTCCAGGGCCAA19000.10885301577280197No Hit
CACCAGGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCG18800.1077071945541409No Hit
CCATCAAGCAGATCACTGTCCTTCTGCCATGGCCCTGTGGATG18660.10690511970107815No Hit
GCTCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACAC18360.10518638787308655No Hit
GTTCAAGGGCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCG18280.10472805938562212No Hit
TTGCAGTAGTTCTCCAGCTGGTAGAGGGAGCAGATGCTGGTAC18160.10404056665442547No Hit
GTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTACTGCAA17940.10278016331389829No Hit
GCCCAGCTCCACCTGCCCCACCTGCAGGTCCTCTGCCTCCCGG17640.1010614314859067No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCATCA24550.024.11405414
CCAGGAC26850.023.9087543
CAGGACA27050.023.7319774
GACAGGC26200.023.2309177
GCATCAG25800.022.87403115
TCCAGGA28100.022.3843422
GCTGCAT26500.022.33962212
CTGCATC27250.021.9284413
GGACAGG28800.021.7760416
ACAGGCT28050.021.5668458
TAGACGG450.003826591420.5555555
ATCAGAA30350.019.93245517
CATCAGA30100.019.91362216
GGCCATC29800.019.86577226
ATCAAGC30200.019.78642530
AGAGGCC30050.019.76206423
AGGCCAT29800.019.7416125
TAACGCT951.6773629E-719.4736844
AAGAGGC30650.019.31484422
TTTAGCG1650.019.06060826
Produced by FastQC (version 0.11.5)