##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630176.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1745473
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.82194797627921	31.0	31.0	34.0	30.0	34.0
2	32.02925453444425	33.0	31.0	34.0	30.0	34.0
3	32.163955558178216	34.0	31.0	34.0	30.0	34.0
4	35.82640292917736	37.0	35.0	37.0	35.0	37.0
5	35.74810094455772	37.0	35.0	37.0	35.0	37.0
6	35.798329736409556	37.0	35.0	37.0	35.0	37.0
7	35.77474300662342	37.0	35.0	37.0	35.0	37.0
8	35.73984186521361	37.0	35.0	37.0	33.0	37.0
9	37.4626556812967	39.0	37.0	39.0	35.0	39.0
10	37.37076597575557	39.0	37.0	39.0	34.0	39.0
11	37.45292995079271	39.0	37.0	39.0	35.0	39.0
12	37.33295788591402	39.0	37.0	39.0	34.0	39.0
13	37.35018989122146	39.0	37.0	39.0	34.0	39.0
14	38.596017812936665	40.0	38.0	41.0	34.0	41.0
15	38.59149296494417	40.0	38.0	41.0	34.0	41.0
16	38.58567849517008	40.0	38.0	41.0	34.0	41.0
17	38.534576014639015	40.0	38.0	41.0	34.0	41.0
18	38.43208173371917	40.0	38.0	41.0	34.0	41.0
19	38.45081992101854	40.0	38.0	41.0	34.0	41.0
20	38.38378365062078	40.0	38.0	41.0	34.0	41.0
21	38.36585842347604	40.0	38.0	41.0	34.0	41.0
22	38.32387438820308	40.0	38.0	41.0	34.0	41.0
23	38.27173665820096	40.0	38.0	41.0	34.0	41.0
24	38.247673839698464	40.0	38.0	41.0	34.0	41.0
25	38.21912684985674	40.0	38.0	41.0	33.0	41.0
26	38.02890047568768	40.0	38.0	41.0	33.0	41.0
27	37.93698670790095	40.0	37.0	41.0	33.0	41.0
28	37.83472159122484	40.0	37.0	41.0	33.0	41.0
29	37.76367036327689	40.0	37.0	41.0	33.0	41.0
30	37.71202877386244	40.0	37.0	41.0	32.0	41.0
31	37.64512427290482	40.0	37.0	41.0	32.0	41.0
32	37.55345685668011	40.0	37.0	41.0	32.0	41.0
33	37.47815806947458	40.0	37.0	41.0	32.0	41.0
34	37.3689074537389	40.0	37.0	41.0	31.0	41.0
35	37.24144687428565	40.0	36.0	41.0	31.0	41.0
36	37.14616897540094	39.0	36.0	41.0	31.0	41.0
37	37.07248522320311	39.0	36.0	41.0	31.0	41.0
38	36.97476443348021	39.0	36.0	41.0	30.0	41.0
39	36.898356491335015	39.0	36.0	41.0	30.0	41.0
40	36.765171961983945	39.0	35.0	41.0	30.0	41.0
41	36.62164467740263	39.0	35.0	40.0	30.0	41.0
42	36.580855160750126	39.0	35.0	40.0	30.0	41.0
43	35.30946740511025	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	3.0
11	2.0
12	1.0
13	2.0
14	2.0
15	3.0
16	15.0
17	36.0
18	81.0
19	182.0
20	398.0
21	693.0
22	1211.0
23	2120.0
24	3410.0
25	5180.0
26	7709.0
27	10831.0
28	15103.0
29	20849.0
30	28117.0
31	36855.0
32	47839.0
33	62215.0
34	83898.0
35	111197.0
36	150581.0
37	226721.0
38	413237.0
39	516978.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.7587404674836	16.361238472322402	11.118934523765192	28.76108653642881
2	19.041142429587854	18.307014774791703	34.200357152473856	28.451485643146583
3	19.965361824559878	20.825587104469676	28.623530699128548	30.585520371841902
4	15.013580845994182	14.01471119862639	32.30402303558978	38.66768491978965
5	15.436961786289446	36.29188191395685	32.842329844116755	15.428826455636955
6	38.27191827086411	32.38657945439431	14.72523493631812	14.616267338423453
7	32.65561827653593	28.8654135583879	19.35549848092752	19.123469684148652
8	29.81627329669379	32.94791726941637	17.904430489615137	19.331378944274704
9	28.341601388276988	13.221860206373858	16.58633505072837	41.85020335462078
10	18.133136404859886	25.78687839915026	30.731612577221192	25.348372618768668
11	38.71403338808449	20.20432283971164	19.62310502654581	21.458538745658053
12	24.790586849524455	22.7126400694826	27.32892459522433	25.167848485768612
13	31.326809409254686	17.22140646117127	23.581630881715157	27.87015324785889
14	23.862414371348052	17.80789505194294	23.73792089594053	34.59176968076848
15	27.088015684000844	25.02198544463306	20.307160294086472	27.582838577279627
16	26.530516370061296	23.97693920215323	20.76205131789492	28.73049310989056
17	25.33427901777914	25.20079084580512	23.67851006575295	25.786420070662796
18	25.845659027667573	22.6921871607295	24.478694313804912	26.98345949779802
19	26.646645350572594	22.73498358324649	23.278045549830907	27.34032551635001
20	26.050761025807905	24.167260106572833	23.37887781707308	26.403101050546184
21	29.117952554980796	22.72467119227854	22.13835447468967	26.019021778050995
22	27.96502724476403	23.631703268970647	22.528449308582832	25.874820177682494
23	26.965183649360373	23.687046433831977	22.329763909267	27.018006007540652
24	27.359231566457915	23.077469545504282	23.117687870279287	26.44561101775851
25	26.94862653275072	22.877237287543263	23.616120100396856	26.55801607930916
26	27.959699176097253	23.077469545504282	23.763931037604134	25.19890024079433
27	28.453834576644844	22.803389110000555	22.087422721520184	26.65535359183442
28	26.3258154093475	22.014262036708672	24.348414441243147	27.311508112700682
29	26.445897473063173	22.259467777502145	24.562110098523437	26.732524650911245
30	25.933371641956075	23.23668140383724	23.994355684676876	26.835591269529807
31	26.039417395743158	23.139229309190114	23.915236729528328	26.906116565538397
32	25.86645568278627	23.021496178972694	23.92165332835283	27.190394809888208
33	27.168337751428982	23.41508576758277	23.11826078088862	26.29831570009963
34	27.604150851946724	23.569026848309885	23.03220960736717	25.794612692376223
35	27.827013078976297	22.936361662426176	24.08922968158201	25.147395577015512
36	27.43886614115486	23.818242963368668	21.688619646365197	27.054271249111274
37	26.150161016526752	23.40591919783348	23.48240276417911	26.961517021460658
38	27.584442726985753	23.179218469721388	23.426028360220982	25.810310443071877
39	25.47280880311526	23.6345678220173	23.85834670602181	27.034276668845635
40	26.638510019920105	22.00761627364044	25.071656794462015	26.28221691197744
41	24.897377387103667	22.484106027420648	25.417236474010195	27.201280111465486
42	25.379309791672515	23.51729302028734	23.875419442179858	27.227977745860294
43	25.781149293056956	20.76205131789492	25.946777750214412	27.510021638833713
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	61.0
1	60.0
2	59.0
3	162.5
4	266.0
5	266.0
6	365.5
7	465.0
8	425.5
9	386.0
10	549.5
11	713.0
12	713.0
13	1287.0
14	1861.0
15	3168.5
16	4476.0
17	4265.5
18	4055.0
19	4055.0
20	4933.5
21	5812.0
22	6017.5
23	6223.0
24	7796.5
25	9370.0
26	9370.0
27	12000.5
28	14631.0
29	18048.5
30	21466.0
31	25814.5
32	30163.0
33	30163.0
34	36410.0
35	42657.0
36	49869.5
37	57082.0
38	63398.5
39	69715.0
40	69715.0
41	76477.0
42	83239.0
43	87575.5
44	91912.0
45	95658.5
46	99405.0
47	99405.0
48	102127.5
49	104850.0
50	113716.0
51	122582.0
52	131557.5
53	140533.0
54	140533.0
55	147363.5
56	154194.0
57	144595.0
58	134996.0
59	120960.0
60	106924.0
61	106924.0
62	106653.0
63	106382.0
64	92473.5
65	78565.0
66	66025.0
67	53485.0
68	53485.0
69	46679.0
70	39873.0
71	37220.5
72	34568.0
73	41548.0
74	48528.0
75	48528.0
76	47095.5
77	45663.0
78	34004.0
79	22345.0
80	13485.5
81	4626.0
82	4626.0
83	3098.5
84	1571.0
85	1214.0
86	857.0
87	673.0
88	489.0
89	489.0
90	409.0
91	329.0
92	197.0
93	65.0
94	45.0
95	25.0
96	25.0
97	15.5
98	6.0
99	3.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1745473.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.06530139159027
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.72560688137233	33.1161638070078
2	11.872732689724193	9.988601578700687
3	3.6549957925495153	4.612454987957691
4	1.6530113588236555	2.7813768405055694
5	0.900369689594632	1.8937161178325386
6	0.5430680606574764	1.3706592988621904
7	0.3795863744870673	1.1177190672857675
8	0.2923199591579833	0.983722174780634
9	0.22558234680354772	0.8540270466222127
>10	1.4413937172841789	11.846695171914162
>50	0.15971425984738127	4.697358126700157
>100	0.12329308426417883	9.906214163631903
>500	0.014504991574860942	4.2628089728126275
>1k	0.013136596143270287	10.442373859875117
>5k	5.473581726362619E-4	1.2960652321466068
>10k+	1.3683954315906548E-4	0.830043553364455
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	14420	0.826137098654634	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	7082	0.4057352935278862	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	5188	0.2972260241206825	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	5149	0.2949916727442934	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	5097	0.2920125375757746	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	4916	0.2816428555468919	No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG	4578	0.26227847695151973	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	4303	0.24652343519492997	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	4055	0.23231525208353265	No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG	3804	0.21793519578933618	No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC	3595	0.20596136405432794	No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG	3511	0.20114891493595147	No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC	3491	0.2000030937172904	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	3464	0.19845623507209792	No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG	3446	0.19742499597530297	No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT	3352	0.19203963624759593	No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT	3252	0.18631053015429055	No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG	3187	0.18258661119364206	No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG	3068	0.17576897494260868	No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT	2976	0.17049819733676774	No Hit
GCACAGGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCC	2669	0.15290984163032026	No Hit
GCCCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGCTGGCCC	2606	0.1493005047915379	No Hit
GGTCCCCAGAGGGCCAGCAGCGCCAGCAGGGGCAGGAGGCGCA	2472	0.1416235026265087	No Hit
CACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACGAGGCT	2451	0.14042039034691456	No Hit
CTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCA	2430	0.13921727806732043	No Hit
CTAGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTG	2377	0.13618085183786857	No Hit
GCTCACACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACG	2325	0.1332017166693498	No Hit
TGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGA	2302	0.13188402226788956	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2273	0.13022258150083102	No Hit
TTGTGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCT	2263	0.12964967089150048	No Hit
GCCATGGCCCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGC	2240	0.12833197649004024	No Hit
GAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGA	2213	0.1267851178448478	No Hit
GTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGTTC	1963	0.11246235261158438	No Hit
CACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCTAGTGT	1946	0.11148840457572247	No Hit
CCACAGGGCCATGGCAGAAGGACAGTGATCTGCTTGATGGCCT	1930	0.1105717476007936	No Hit
TTCTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGC	1921	0.11005612805239612	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1907	0.10925405319933336	No Hit
GTTCCACAATGCCACGCTTCTGCAGGGACCCCTCCAGGGCCAA	1900	0.10885301577280197	No Hit
CACCAGGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCG	1880	0.1077071945541409	No Hit
CCATCAAGCAGATCACTGTCCTTCTGCCATGGCCCTGTGGATG	1866	0.10690511970107815	No Hit
GCTCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACAC	1836	0.10518638787308655	No Hit
GTTCAAGGGCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCG	1828	0.10472805938562212	No Hit
TTGCAGTAGTTCTCCAGCTGGTAGAGGGAGCAGATGCTGGTAC	1816	0.10404056665442547	No Hit
GTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTACTGCAA	1794	0.10278016331389829	No Hit
GCCCAGCTCCACCTGCCCCACCTGCAGGTCCTCTGCCTCCCGG	1764	0.1010614314859067	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	2.864553046652684E-4	0.0	0.0	0.0	0.0
6	2.864553046652684E-4	0.0	0.0	0.0	0.0
7	2.864553046652684E-4	0.0	0.0	0.0	0.0
8	2.864553046652684E-4	0.0	0.0	5.729106093305368E-5	0.0
9	2.864553046652684E-4	0.0	0.0	1.1458212186610736E-4	0.0
10	3.4374636559832204E-4	5.729106093305368E-5	0.0	1.7187318279916102E-4	0.0
11	5.729106093305368E-4	5.729106093305368E-5	0.0	1.7187318279916102E-4	0.0
12	5.729106093305368E-4	5.729106093305368E-5	0.0	2.291642437322147E-4	0.0
13	5.729106093305368E-4	5.729106093305368E-5	0.0	2.864553046652684E-4	0.0
14	5.729106093305368E-4	5.729106093305368E-5	0.0	2.864553046652684E-4	0.0
15	6.302016702635904E-4	5.729106093305368E-5	0.0	3.4374636559832204E-4	0.0
16	6.302016702635904E-4	5.729106093305368E-5	0.0	5.156195483974831E-4	5.729106093305368E-5
17	6.874927311966441E-4	5.729106093305368E-5	0.0	5.729106093305368E-4	5.729106093305368E-5
18	8.020748530627515E-4	5.729106093305368E-5	0.0	6.302016702635904E-4	5.729106093305368E-5
19	8.020748530627515E-4	5.729106093305368E-5	0.0	8.593659139958051E-4	5.729106093305368E-5
20	8.020748530627515E-4	5.729106093305368E-5	0.0	9.166569749288588E-4	5.729106093305368E-5
21	8.593659139958051E-4	5.729106093305368E-5	0.0	0.0010885301577280198	5.729106093305368E-5
22	9.166569749288588E-4	5.729106093305368E-5	0.0	0.0015468586451924493	5.729106093305368E-5
23	9.739480358619125E-4	1.1458212186610736E-4	0.0	0.0023489334982552007	5.729106093305368E-5
24	0.0010312390967949661	1.1458212186610736E-4	0.0	0.0034374636559832204	5.729106093305368E-5
25	0.0010885301577280198	1.1458212186610736E-4	0.0	0.004296829569979026	5.729106093305368E-5
26	0.0010885301577280198	1.1458212186610736E-4	0.0	0.005843688215171475	5.729106093305368E-5
27	0.0010885301577280198	1.1458212186610736E-4	0.0	0.011916540674075165	1.1458212186610736E-4
28	0.0011458212186610736	1.7187318279916102E-4	0.0	0.029676769563321805	1.1458212186610736E-4
29	0.0012031122795941273	1.7187318279916102E-4	0.0	0.05591607547066039	1.1458212186610736E-4
30	0.0012604033405271809	1.7187318279916102E-4	0.0	0.09418650417394024	1.1458212186610736E-4
31	0.0012604033405271809	1.7187318279916102E-4	0.0	0.19805519764556656	1.1458212186610736E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCATCA	2455	0.0	24.114054	14
CCAGGAC	2685	0.0	23.908754	3
CAGGACA	2705	0.0	23.731977	4
GACAGGC	2620	0.0	23.230917	7
GCATCAG	2580	0.0	22.874031	15
TCCAGGA	2810	0.0	22.384342	2
GCTGCAT	2650	0.0	22.339622	12
CTGCATC	2725	0.0	21.92844	13
GGACAGG	2880	0.0	21.776041	6
ACAGGCT	2805	0.0	21.566845	8
TAGACGG	45	0.0038265914	20.555555	5
ATCAGAA	3035	0.0	19.932455	17
CATCAGA	3010	0.0	19.913622	16
GGCCATC	2980	0.0	19.865772	26
ATCAAGC	3020	0.0	19.786425	30
AGAGGCC	3005	0.0	19.762064	23
AGGCCAT	2980	0.0	19.74161	25
TAACGCT	95	1.6773629E-7	19.473684	4
AAGAGGC	3065	0.0	19.314844	22
TTTAGCG	165	0.0	19.060608	26
>>END_MODULE