FastQCFastQC Report
Fri 10 Feb 2017
ERR1630175.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630175.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1167819
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC78190.6695386870739387No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT43390.3715473031351605No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT32310.2766695866397104No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG29560.2531214169319047No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG29360.251408822771337No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG25530.2186126445964657No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG24760.21201915707828012No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT23500.20122981386670366No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT22910.19617766109302895No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG22740.19472195605654644No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC22290.19086861919526912No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG21690.18573083671356605No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC20990.17973675715157914No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT20870.17870920065523851No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT20810.17819542240706823No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG20490.1754552717501599No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG20380.17451334496184767No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT19770.16928993277211624No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG18430.1578155518963127No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT16550.1417171667869764No Hit
TGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGA15290.13092782357539995No Hit
CTAGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTG15130.1295577482469458No Hit
CACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACGAGGCT14590.12493374401341305No Hit
GGTCCCCAGAGGGCCAGCAGCGCCAGCAGGGGCAGGAGGCGCA13820.11834025649522742No Hit
TTGTGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCT13550.11602825437846105No Hit
GCACAGGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCC13520.1157713652543759No Hit
GCTCACACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACG13410.11482943846606367No Hit
CTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCA13370.11448691963395012No Hit
GAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGA13330.11414440080183659No Hit
GTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGTTC12420.1063520973712536No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12340.10566705970702651No Hit
TTGCAGTAGTTCTCCAGCTGGTAGAGGGAGCAGATGCTGGTAC12330.10558142999899814No Hit
TTCTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGC11810.10112868518152214No Hit
CACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCTAGTGT11760.1007005366413802No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGCGG405.9402108E-527.7510
GGTATCA5250.022.9047621
TCTATCG705.102038E-621.14285929
CAGGACA18950.020.4036944
TACGGTG555.1431626E-420.18181828
TGCATCA18100.020.0331514
CCAGGAC19550.019.9667533
GCTGCAT18650.019.6407512
ATTAGAC851.2453438E-619.5882343
TCCAGGA19550.019.4936072
CTATATC951.6761624E-719.4736842
GCATCAG19200.019.27083415
ACAGGCT19150.019.2245438
TATTAGA1355.638867E-1119.1851862
GACAGGC19200.019.174487
CTGCATC19400.018.78608313
CAACCGT500.007034947618.56
TACGGTC500.007034947618.510
ACGGACC1800.018.58
CATAATA1501.2732926E-1118.52
Produced by FastQC (version 0.11.5)