FastQCFastQC Report
Fri 10 Feb 2017
ERR1630174.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630174.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2456945
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT105140.42792980713853995No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT100950.4108761083377935No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT79770.32467149244285076No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG77780.316572003036291No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG60850.24766529165284532No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT52940.215470838785565No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52150.21225546359401617No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG49750.20248723516399428No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC46020.1873057801456687No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC45690.1859626487365407No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC45350.18457881637562096No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA43980.17900278598015015No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC39470.16064665672206746No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA34370.13988917130827105No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT33610.13679589897209748No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC33510.13638888945417987No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA33410.1359818799362623No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT32860.13374332758771565No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT32530.13240019617858764No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG31950.1300395409746657No Hit
GTATTTACAGTTTTCAGTTTCTAATGCAAGGGTCTCGCTGAAG30170.12279477155573283No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG29310.11929448970164166No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA29300.11925378874984992No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG29110.11848047066580653No Hit
ATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTGCG28880.1175443487745961No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA28800.11721874116026204No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG28140.11453247834200603No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC27960.1137998612097544No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA27770.113026543125711No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG27650.11253813170420991No Hit
GATCTGCTAACTCAAACCCGGCTATGGCACCCCGAGAACGCAA26450.10765401748919898No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA26380.10736911082665668No Hit
GTGTGGGGGCGAGGGATCAGAGGTCTGATATGGACAATACTAG26090.10618878322469572No Hit
GATCAGAGGTCTGATATGGACAATACTAGTTAAGAAAGCTAAC25270.10285130517777158No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC24910.1013860709132683No Hit
AGGGAAGAGAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTG24670.10040924807026613No Hit
TTCTAATGCAAGGGTCTCGCTGAAGACTTGGAGGATTAGGGAA24660.10036854711847436No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAC502.7033145E-422.228
TCTAGCG1601.8189894E-1218.528
CTAGCGG1900.017.52631629
ATACGCC752.0684724E-417.2666666
GGTATCA44150.017.2219721
CTTATAC14400.016.57291637
CGAACGC700.002593620215.85714230
CGCAATA2109.094947E-1214.9761936
ATGGTCG2101.364242E-1014.09523836
GCTTTAC2009.858923E-1013.8750011
TAACGGC800.00630131213.87536
TATGACG800.00630131213.8754
TCGTTTA2405.456968E-1213.87499930
CGGTCCA2304.0017767E-1113.67391310
TCTTATA23500.013.46170137
CGAGCCG2800.013.21428515
TTAACGG850.0094087113.05882435
AATACGC1000.001833511712.9500015
TAGCGGC3000.012.9530
GTATTAG5200.012.8076931
Produced by FastQC (version 0.11.5)