FastQCFastQC Report
Fri 10 Feb 2017
ERR1630173.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630173.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1553833
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT74090.47682086813705205No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT60450.3890379468063814No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT47210.30382930469361896No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22330.14370913733972698No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT18690.12028319645676208No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG451.3235054E-424.6666645
ATACCGA451.3235054E-424.6666646
CAAGACG4900.019.6326524
TTCGGGC2550.018.86274535
GGTATCA22500.018.7466681
ACCGACC1002.8765498E-718.58
AAGACGG5000.018.1300015
CGCAAGA5000.018.1300012
CTTATAC6950.018.1007237
GACGGAC5200.017.7884627
CGAACGA2300.017.69565216
CGGACCA5150.017.6019429
ACGGACC5400.017.129638
GCGCAAG5300.017.1037731
TCGTAGC650.001580272217.0769232
CGAGCCG3800.017.03947415
ATAACGA2600.016.36538512
CGGTCCA2400.016.187510
TACCACG1151.2428063E-616.0869564
GCTTCGG3000.016.03333333
Produced by FastQC (version 0.11.5)