##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630173.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1553833
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.251356484255385	31.0	31.0	34.0	28.0	34.0
2	31.462511737104307	31.0	31.0	34.0	30.0	34.0
3	31.59550479362969	31.0	31.0	34.0	30.0	34.0
4	35.36017834606422	37.0	35.0	37.0	33.0	37.0
5	35.170271193879906	37.0	35.0	37.0	32.0	37.0
6	35.24144872711546	37.0	35.0	37.0	32.0	37.0
7	35.23164908970269	37.0	35.0	37.0	32.0	37.0
8	35.20470925768728	37.0	35.0	37.0	32.0	37.0
9	36.81219024180848	38.0	37.0	39.0	33.0	39.0
10	36.64798984189421	38.0	35.0	39.0	32.0	39.0
11	36.76334844220711	38.0	37.0	39.0	32.0	39.0
12	36.618394640865525	38.0	35.0	39.0	32.0	39.0
13	36.67761464713389	38.0	35.0	39.0	32.0	39.0
14	37.718976878467636	39.0	37.0	40.0	33.0	41.0
15	37.68948593574728	39.0	37.0	40.0	33.0	41.0
16	37.655707530989496	39.0	37.0	40.0	32.0	41.0
17	37.618242758391666	39.0	37.0	40.0	32.0	41.0
18	37.583758357558374	39.0	37.0	40.0	32.0	41.0
19	37.59377809584428	39.0	37.0	40.0	32.0	41.0
20	37.55566589202314	39.0	37.0	40.0	32.0	41.0
21	37.491597230847844	39.0	37.0	40.0	32.0	41.0
22	37.45516152636738	39.0	36.0	40.0	32.0	41.0
23	37.378165478529546	39.0	36.0	40.0	32.0	41.0
24	37.354899786527895	39.0	36.0	40.0	32.0	41.0
25	37.317233576581266	39.0	36.0	40.0	32.0	41.0
26	37.1254491312773	39.0	36.0	40.0	31.0	41.0
27	37.00332661231934	39.0	36.0	40.0	31.0	41.0
28	36.87973417992796	39.0	36.0	40.0	31.0	41.0
29	36.79577535037549	39.0	36.0	40.0	30.0	41.0
30	36.71297301576167	39.0	36.0	40.0	30.0	41.0
31	36.576018143519924	38.0	35.0	40.0	30.0	41.0
32	36.45160966461647	38.0	35.0	40.0	30.0	41.0
33	36.36048532886095	38.0	35.0	40.0	30.0	41.0
34	36.25310313270474	38.0	35.0	40.0	30.0	41.0
35	36.13297310586144	38.0	35.0	40.0	30.0	41.0
36	36.00811992022309	38.0	35.0	40.0	29.0	41.0
37	35.95054552194477	38.0	35.0	40.0	29.0	41.0
38	35.805246123618176	38.0	35.0	40.0	28.0	41.0
39	35.69768758933554	38.0	34.0	40.0	28.0	41.0
40	35.563969229640506	38.0	34.0	40.0	27.0	41.0
41	35.424288839276805	38.0	34.0	40.0	27.0	41.0
42	35.33426114646812	38.0	34.0	40.0	26.0	41.0
43	34.139096028981236	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	6.0
11	7.0
12	4.0
13	6.0
14	12.0
15	17.0
16	42.0
17	88.0
18	171.0
19	331.0
20	651.0
21	1119.0
22	1920.0
23	3085.0
24	4887.0
25	7458.0
26	10912.0
27	15275.0
28	20874.0
29	27864.0
30	36572.0
31	46349.0
32	59512.0
33	75694.0
34	97343.0
35	129092.0
36	180741.0
37	267312.0
38	380638.0
39	185850.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.01109964841781	20.710462449954402	13.699026858098652	24.57941104352913
2	18.101623533545755	22.138479489108544	35.42388403386979	24.336012943475907
3	19.683646826911257	23.805067854782337	30.76508221926037	25.74620309904604
4	14.667277628934382	16.354202800429647	35.19207019029715	33.78644938033881
5	13.861206448826868	37.708363768822004	34.31211719663567	14.118312585715454
6	33.317608777777274	36.50881401025722	15.9165109764048	14.257066235560705
7	28.406205814910614	31.955300215660245	21.268887969299147	18.369606000130002
8	26.790266392849166	34.78456179010228	20.166839036112634	18.25833278093592
9	26.42278803449277	14.603757289232497	20.369370453581563	38.604084222693174
10	17.014891561705795	27.41034589946281	32.678029106087976	22.896733432743417
11	35.44151784651246	22.52745307893448	21.38099782923905	20.650031245314008
12	21.013455113902204	26.78119205860604	29.412620275151834	22.792732552339924
13	29.628151802671198	19.86114337898603	25.805668948979715	24.70503586936305
14	22.343649542775832	21.696926246256837	25.670583647019985	30.28884056394735
15	24.882017565594243	28.56639033924495	22.761004560979202	23.7905875341816
16	24.177630414594102	27.381385258261343	24.419677018057925	24.021307309086627
17	22.751222299951156	27.38093475939821	25.995908183183136	23.8719347574675
18	22.72773200208774	25.530285429643985	27.898493596158662	23.84348897210962
19	23.269939562359664	26.114839883050493	27.77055191902862	22.84466863556122
20	24.110827868889384	25.320932172247595	27.33607794402616	23.232162014836856
21	24.782521673822092	26.094116935346335	26.160018483324787	22.963342907506792
22	24.181041334557833	25.789129205004656	26.803266502899604	23.226562957537908
23	23.219548046669107	25.815579923968663	27.071570754386087	23.89330127497614
24	23.615600904344287	26.204617870775042	27.076204456978324	23.103576767902343
25	23.759374398664466	25.68654417817101	26.90739609726399	23.64668532590053
26	23.27605347550219	26.71033502313312	26.92316355747368	23.09044794389101
27	23.95534140412773	25.751222943520958	26.615279763011856	23.678155889339457
28	23.019590908418085	26.18614741738655	27.559589737121044	23.23467193707432
29	22.727667645107292	26.527239413759396	27.507460582958398	23.237632358174913
30	22.71267246866298	27.247394024969225	27.32764717958751	22.71228632678029
31	23.652413097160377	26.727711407854	26.66245342968002	22.957422065305604
32	22.704563489126567	26.37162423503684	26.65196324186705	24.271849033969545
33	22.446170212629028	26.10492890806155	27.647565729393055	23.80133514991637
34	23.49441671016126	25.653722118142685	27.327968964489752	23.52389220720631
35	23.58561055145566	25.82935231778447	27.704264229167485	22.880772901592387
36	22.61774592250261	26.615022335090067	27.296627115011717	23.47060462739561
37	23.73260189479822	25.681459976715644	27.1174572814453	23.468480847040833
38	22.788999847473956	25.841515787089087	27.98132102999486	23.388163335442098
39	23.209637071680163	25.333288712493555	27.79623035422726	23.660843861599027
40	22.572181180345634	25.38985849830709	28.638663228287726	23.399297093059552
41	21.92294796158918	25.747361524694096	28.936571690780156	23.39311882293657
42	22.88534224720417	25.329942149510277	28.67405956753396	23.11065603575159
43	22.136355708753772	24.959052871190146	28.624826477491467	24.279764942564615
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	265.0
1	234.5
2	204.0
3	474.5
4	745.0
5	745.0
6	1086.5
7	1428.0
8	1467.0
9	1506.0
10	2134.5
11	2763.0
12	2763.0
13	4812.0
14	6861.0
15	11630.0
16	16399.0
17	15438.0
18	14477.0
19	14477.0
20	16635.0
21	18793.0
22	18827.5
23	18862.0
24	21843.0
25	24824.0
26	24824.0
27	28481.0
28	32138.0
29	38937.0
30	45736.0
31	49417.0
32	53098.0
33	53098.0
34	57881.5
35	62665.0
36	68516.0
37	74367.0
38	79950.5
39	85534.0
40	85534.0
41	90816.0
42	96098.0
43	94774.5
44	93451.0
45	96810.5
46	100170.0
47	100170.0
48	103103.0
49	106036.0
50	105033.0
51	104030.0
52	108885.5
53	113741.0
54	113741.0
55	108380.5
56	103020.0
57	98069.5
58	93119.0
59	85998.0
60	78877.0
61	78877.0
62	71646.0
63	64415.0
64	55857.0
65	47299.0
66	40069.0
67	32839.0
68	32839.0
69	27667.0
70	22495.0
71	19399.0
72	16303.0
73	12759.5
74	9216.0
75	9216.0
76	6868.5
77	4521.0
78	3667.5
79	2814.0
80	2230.0
81	1646.0
82	1646.0
83	1286.0
84	926.0
85	850.0
86	774.0
87	737.5
88	701.0
89	701.0
90	614.0
91	527.0
92	317.0
93	107.0
94	69.5
95	32.0
96	32.0
97	19.0
98	6.0
99	5.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1553833.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.93100462797831
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.98057649542724	46.97118012626028
2	10.076325688799885	11.27158037466566
3	2.568419031395729	4.309627701947464
4	0.9872343679712721	2.208680400156018
5	0.528534846677197	1.478074247777505
6	0.3258267854427531	1.093429166671075
7	0.2158611535520725	0.8451331824825192
8	0.15651410868758034	0.7003193069879164
9	0.12020395554788385	0.6050815194644992
>10	0.8082344925158205	8.971355193593896
>50	0.1172257776824313	4.6025268328497
>100	0.10581221740486241	11.906342068645673
>500	0.007154086143854034	2.627920360607744
>1k	0.0018462157790591056	1.5404560791607418
>5k	2.307769723823882E-4	0.8682934387292565
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7409	0.47682086813705205	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6045	0.3890379468063814	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4721	0.30382930469361896	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2233	0.14370913733972698	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	1869	0.12028319645676208	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	6.435698044770578E-5	0.0	0.0	0.0
15	0.0	6.435698044770578E-5	0.0	6.435698044770578E-5	0.0
16	0.0	6.435698044770578E-5	0.0	5.79212824029352E-4	0.0
17	0.0	6.435698044770578E-5	0.0	7.722837653724692E-4	0.0
18	0.0	6.435698044770578E-5	0.0	7.722837653724692E-4	0.0
19	0.0	6.435698044770578E-5	0.0	0.0010297116871632924	0.0
20	0.0	6.435698044770578E-5	0.0	0.0012227826285064096	0.0
21	0.0	6.435698044770578E-5	0.0	0.00167328149164035	0.0
22	0.0	6.435698044770578E-5	0.0	0.0028960641201467596	0.0
23	0.0	6.435698044770578E-5	0.0	0.004376274670443992	0.0
24	0.0	6.435698044770578E-5	0.0	0.006564412005665989	0.0
25	0.0	6.435698044770578E-5	0.0	0.007207981810143046	0.0
26	0.0	6.435698044770578E-5	0.0	0.009009977262678808	0.0
27	0.0	6.435698044770578E-5	0.0	0.01551003228789709	0.0
28	0.0	6.435698044770578E-5	0.0	0.04009439881892069	6.435698044770578E-5
29	0.0	6.435698044770578E-5	0.0	0.07793630332217169	6.435698044770578E-5
30	0.0	6.435698044770578E-5	0.0	0.1347635170574959	6.435698044770578E-5
31	0.0	6.435698044770578E-5	0.0	0.34604748386731393	6.435698044770578E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	45	1.3235054E-4	24.666664	5
ATACCGA	45	1.3235054E-4	24.666664	6
CAAGACG	490	0.0	19.632652	4
TTCGGGC	255	0.0	18.862745	35
GGTATCA	2250	0.0	18.746668	1
ACCGACC	100	2.8765498E-7	18.5	8
AAGACGG	500	0.0	18.130001	5
CGCAAGA	500	0.0	18.130001	2
CTTATAC	695	0.0	18.10072	37
GACGGAC	520	0.0	17.788462	7
CGAACGA	230	0.0	17.695652	16
CGGACCA	515	0.0	17.601942	9
ACGGACC	540	0.0	17.12963	8
GCGCAAG	530	0.0	17.103773	1
TCGTAGC	65	0.0015802722	17.076923	2
CGAGCCG	380	0.0	17.039474	15
ATAACGA	260	0.0	16.365385	12
CGGTCCA	240	0.0	16.1875	10
TACCACG	115	1.2428063E-6	16.086956	4
GCTTCGG	300	0.0	16.033333	33
>>END_MODULE