FastQCFastQC Report
Fri 10 Feb 2017
ERR1630172.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630172.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25517
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3161.238390092879257No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2871.1247403691656543No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1500.5878433985186347No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1320.5173021906963985No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA690.2704079633185719No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT630.24689422737782651No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA590.23121840341732963No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA590.23121840341732963No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA570.22338049143708116No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA500.19594779950621155No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA500.19594779950621155No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA470.18419093153583885No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA420.1645961515852177No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT400.15675823960496924No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA390.152839283614845No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG390.152839283614845No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT380.14892032762472077No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA370.14500137163459653No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA360.1410824156444723No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT350.1371634596543481No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT330.12932554767409962No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC330.12932554767409962No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA320.1254065916839754No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.12148763569385117No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC300.11756867970372693No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC300.11756867970372693No Hit
CCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA290.1136497237136027No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG290.1136497237136027No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC280.10973076772347846No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC280.10973076772347846No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270.10581181173335424No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC260.10189285574323001No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC260.10189285574323001No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGATT250.005397392429.615
ACAAAAA250.005397392429.623
CAGTCGG250.005397392429.610
TCGGTGA250.005397392429.613
GCAGTCG250.005397392429.69
AGTCGGT250.005397392429.611
CGGTGAT250.005397392429.614
GTCGGTG250.005397392429.612
TGGGAAC551.8098986E-523.54545420
TCTTATA1053.45608E-1122.9047637
GGGAACA608.903752E-418.521
CTCTTAT1401.5970727E-917.17857236
TCTATGG750.00395996814.82
AGTACTT1507.093513E-814.812
GAGTACT1507.093513E-814.811
TCTCTTA1753.058267E-813.74285835
GGTATCA3150.013.5079371
ATGGGAA2408.9858077E-1012.33333419
CATGGGA2408.9858077E-1012.33333418
GTACTTT1806.556347E-712.33333313
Produced by FastQC (version 0.11.5)