##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630172.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	25517
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.077477759924758	31.0	31.0	34.0	28.0	34.0
2	31.283928361484502	31.0	31.0	34.0	28.0	34.0
3	31.216835834933573	31.0	31.0	34.0	28.0	34.0
4	35.09370223772387	37.0	35.0	37.0	32.0	37.0
5	34.98467688207862	35.0	35.0	37.0	32.0	37.0
6	35.063839793079126	36.0	35.0	37.0	32.0	37.0
7	35.07540071324999	36.0	35.0	37.0	32.0	37.0
8	35.0819845593134	37.0	35.0	37.0	32.0	37.0
9	36.5834933573696	38.0	35.0	39.0	32.0	39.0
10	36.45013128502567	38.0	35.0	39.0	32.0	39.0
11	36.576204099227965	38.0	35.0	39.0	32.0	39.0
12	36.40090919778971	38.0	35.0	39.0	32.0	39.0
13	36.45926245248266	38.0	35.0	39.0	32.0	39.0
14	37.31927734451542	39.0	36.0	40.0	32.0	41.0
15	37.32915311361054	39.0	36.0	40.0	32.0	41.0
16	37.08715758122036	39.0	36.0	40.0	32.0	41.0
17	37.25919974918682	39.0	36.0	40.0	32.0	41.0
18	37.32660579221695	39.0	36.0	40.0	32.0	41.0
19	37.34228161617745	39.0	36.0	40.0	32.0	41.0
20	37.33777481678881	39.0	36.0	40.0	32.0	41.0
21	37.27624720774386	39.0	36.0	40.0	32.0	41.0
22	37.23744954344163	39.0	36.0	40.0	32.0	41.0
23	37.21068307402908	39.0	36.0	40.0	32.0	41.0
24	37.103891523298195	39.0	36.0	40.0	31.0	41.0
25	37.019046126112	39.0	36.0	40.0	31.0	41.0
26	36.76223694007916	39.0	35.0	40.0	31.0	41.0
27	36.61084767018066	38.0	35.0	40.0	30.0	41.0
28	36.37966845632324	38.0	35.0	40.0	30.0	41.0
29	36.30951914410001	38.0	35.0	40.0	30.0	41.0
30	36.124505231806246	38.0	35.0	40.0	30.0	41.0
31	35.88787866912254	38.0	35.0	40.0	30.0	41.0
32	35.71583650115609	38.0	34.0	40.0	29.0	41.0
33	35.653485911353215	38.0	34.0	40.0	29.0	41.0
34	35.50578046008543	38.0	34.0	40.0	28.0	41.0
35	35.36152369008896	38.0	34.0	40.0	27.0	41.0
36	35.19806403574088	38.0	34.0	40.0	27.0	41.0
37	35.04647881804287	38.0	34.0	40.0	26.0	41.0
38	34.899243641493904	38.0	33.0	40.0	26.0	41.0
39	34.69796606184113	38.0	33.0	40.0	25.0	41.0
40	34.44958263118705	37.0	33.0	40.0	24.0	41.0
41	34.2271426891876	37.0	33.0	40.0	23.0	41.0
42	34.01939883215112	37.0	33.0	40.0	23.0	41.0
43	32.77673707724262	36.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	7.0
20	9.0
21	27.0
22	47.0
23	76.0
24	84.0
25	152.0
26	252.0
27	336.0
28	442.0
29	617.0
30	757.0
31	954.0
32	1158.0
33	1394.0
34	1782.0
35	2328.0
36	3171.0
37	4349.0
38	5431.0
39	2143.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.641493906023435	20.02586510953482	15.185954461731393	21.14668652271035
2	21.14668652271035	22.792648038562525	32.46855037817925	23.59211506054787
3	21.389661794098053	24.195634283027	32.6057138378336	21.808990085041344
4	15.362307481286985	19.60653681859153	32.74287729748795	32.28827840263354
5	14.151350080338599	36.64223850766156	33.51883058353255	15.687580828467295
6	30.258259199749187	33.59720970333503	17.20029784065525	18.94423325626053
7	25.351726300113647	32.993690480855896	21.083983226868362	20.57059999216209
8	28.298781204687074	32.053141043226084	20.77830465963867	18.86977309244817
9	26.34322216561508	13.120664654935926	20.45303131245836	40.08308186699063
10	22.004937884547555	26.515656229180546	27.260257867304148	24.219148018967747
11	33.04463690872751	22.98075792608849	18.12517145432457	25.849433710859426
12	20.135595877258297	29.944742720539246	27.150527099580675	22.769134302621783
13	33.58153387937454	18.39557941764314	25.9748403025434	22.048046400438924
14	22.871027158365013	23.44319473292315	26.66457655680527	27.02120155190657
15	30.17596112395658	24.481718070306073	23.38049143708116	21.96182936865619
16	21.63655602147588	25.383077948034643	28.05580593329937	24.92456009719011
17	20.60978955206333	29.047301798800802	25.551593055609985	24.791315593525884
18	20.41384175255712	22.882784026335383	29.207978994395894	27.495395226711604
19	22.29494062781675	27.471881490770862	28.561351256025397	21.671826625387
20	23.725359564212095	21.80507112905122	32.343143786495276	22.12642552024141
21	26.974174080025083	23.059137045890974	28.14986087706235	21.816827997021594
22	26.276599913782967	24.39941999451346	26.86836226829173	22.455617823411846
23	25.89254222675079	24.524826586197438	27.362150723047378	22.22048046400439
24	23.00819061801936	26.276599913782967	28.330132852608063	22.385076615589607
25	23.349139789160166	25.58294470353098	28.51824274013403	22.549672767174826
26	25.477132891797627	24.579691970059176	27.511071050672104	22.432104087471096
27	23.650899400399734	25.175373280558063	27.534584786612847	23.63914253242936
28	22.243994199945135	26.45295293333856	29.568522945487324	21.734529921228983
29	23.74887330015284	25.535917231649492	28.800407571422976	21.914801896774698
30	22.439941999451346	27.440529842849866	30.179880079946702	19.939648077752086
31	23.662656268370107	25.488889759767996	27.989183681467257	22.85927029039464
32	19.994513461613824	26.67633342477564	29.047301798800802	24.281851314809735
33	21.213308774542462	25.818082062938437	30.583532546929497	22.385076615589607
34	20.44519340047811	26.605792216953404	28.537837520084647	24.411176862483835
35	21.63655602147588	26.723360896657127	29.01203119488968	22.62805188697731
36	20.33938158874476	26.362816945565704	28.416349884390797	24.88145158129874
37	21.205470862562215	27.060391111807814	28.388917192459928	23.345220833170043
38	20.51573460830035	24.67766586981228	29.356899322020613	25.449700199866754
39	21.170200258651096	23.772387036093583	30.983266057922172	24.07414664733315
40	20.23748873300153	24.857937845358	31.759219343966766	23.14535407767371
41	19.29693929537171	25.100913116745698	31.720029784065524	23.882117803817064
42	18.881529960418543	24.724693341693772	32.82909432927068	23.564682368617003
43	18.3563898577419	26.65673864482502	31.44178390876671	23.54508758866638
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	4.0
2	5.0
3	16.5
4	28.0
5	28.0
6	48.0
7	68.0
8	71.5
9	75.0
10	95.5
11	116.0
12	116.0
13	238.0
14	360.0
15	519.5
16	679.0
17	541.5
18	404.0
19	404.0
20	484.0
21	564.0
22	390.5
23	217.0
24	212.0
25	207.0
26	207.0
27	213.5
28	220.0
29	241.0
30	262.0
31	289.5
32	317.0
33	317.0
34	409.0
35	501.0
36	523.5
37	546.0
38	631.0
39	716.0
40	716.0
41	951.0
42	1186.0
43	1519.5
44	1853.0
45	2310.0
46	2767.0
47	2767.0
48	3108.5
49	3450.0
50	2967.0
51	2484.0
52	2272.0
53	2060.0
54	2060.0
55	1941.5
56	1823.0
57	1624.5
58	1426.0
59	1231.5
60	1037.0
61	1037.0
62	896.5
63	756.0
64	636.5
65	517.0
66	411.0
67	305.0
68	305.0
69	260.5
70	216.0
71	184.0
72	152.0
73	124.5
74	97.0
75	97.0
76	70.0
77	43.0
78	39.5
79	36.0
80	23.5
81	11.0
82	11.0
83	9.0
84	7.0
85	4.5
86	2.0
87	1.5
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	25517.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.76208018183955
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.82859818919762	51.98495120899792
2	8.585700905401186	10.777128972841634
3	3.2344676865438653	6.090057608653055
4	1.667187012176085	4.185444997452679
5	1.0427724008741805	3.2723282517537324
6	0.5057758351545426	1.9046126112003763
7	0.39338120512019986	1.728259591644786
8	0.2684982828598189	1.3481208606027355
9	0.20605682172962847	1.1639299290668965
>10	1.2113643459256946	12.873770427558098
>50	0.031220730565095226	1.203119488968139
>100	0.024976584452076178	3.468276051259944
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	316	1.238390092879257	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	287	1.1247403691656543	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	150	0.5878433985186347	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	132	0.5173021906963985	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	69	0.2704079633185719	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	63	0.24689422737782651	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	59	0.23121840341732963	No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA	59	0.23121840341732963	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	57	0.22338049143708116	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	50	0.19594779950621155	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	50	0.19594779950621155	No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA	47	0.18419093153583885	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	42	0.1645961515852177	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT	40	0.15675823960496924	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	39	0.152839283614845	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	39	0.152839283614845	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	38	0.14892032762472077	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	37	0.14500137163459653	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	36	0.1410824156444723	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35	0.1371634596543481	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	33	0.12932554767409962	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	33	0.12932554767409962	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	32	0.1254065916839754	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	31	0.12148763569385117	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	30	0.11756867970372693	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	30	0.11756867970372693	No Hit
CCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA	29	0.1136497237136027	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	29	0.1136497237136027	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	28	0.10973076772347846	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	28	0.10973076772347846	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27	0.10581181173335424	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	26	0.10189285574323001	No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC	26	0.10189285574323001	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.023513735940745385	0.0
17	0.0	0.0	0.0	0.03527060391111808	0.0
18	0.0	0.0	0.0	0.03527060391111808	0.0
19	0.0	0.0	0.0	0.050946427871615005	0.0
20	0.0	0.0	0.0	0.050946427871615005	0.0
21	0.0	0.0	0.0	0.07837911980248462	0.0
22	0.0	0.0	0.0	0.12148763569385115	0.0
23	0.0	0.0	0.0	0.14500137163459653	0.0
24	0.0	0.0	0.0	0.22338049143708116	0.0
25	0.0	0.0	0.0	0.23905631539757807	0.0
26	0.0	0.0	0.0	0.26257005133832345	0.0
27	0.0	0.0	0.0	0.3017596112395658	0.0
28	0.0	0.0	0.0	0.4036524669827958	0.0
29	0.0	0.0	0.0	0.5055453227260258	0.0
30	0.0	0.0	0.0	0.7485205941137281	0.0
31	0.0	0.0	0.0	1.3833914645138534	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTGATT	25	0.0053973924	29.6	15
ACAAAAA	25	0.0053973924	29.6	23
CAGTCGG	25	0.0053973924	29.6	10
TCGGTGA	25	0.0053973924	29.6	13
GCAGTCG	25	0.0053973924	29.6	9
AGTCGGT	25	0.0053973924	29.6	11
CGGTGAT	25	0.0053973924	29.6	14
GTCGGTG	25	0.0053973924	29.6	12
TGGGAAC	55	1.8098986E-5	23.545454	20
TCTTATA	105	3.45608E-11	22.90476	37
GGGAACA	60	8.903752E-4	18.5	21
CTCTTAT	140	1.5970727E-9	17.178572	36
TCTATGG	75	0.003959968	14.8	2
AGTACTT	150	7.093513E-8	14.8	12
GAGTACT	150	7.093513E-8	14.8	11
TCTCTTA	175	3.058267E-8	13.742858	35
GGTATCA	315	0.0	13.507937	1
ATGGGAA	240	8.9858077E-10	12.333334	19
CATGGGA	240	8.9858077E-10	12.333334	18
GTACTTT	180	6.556347E-7	12.333333	13
>>END_MODULE