FastQCFastQC Report
Fri 10 Feb 2017
ERR1630171.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630171.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151348
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT41802.761846869466395No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT34062.250442688373814No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25941.7139308084679017No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14740.9739144223907816No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5450.36009725929645586No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4910.3244178978248804No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA4490.2966672833469884No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA3720.24579115680418637No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3610.2385231387266432No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3420.22596928931997778No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3100.20482596400348863No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2380.15725348204138806No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1840.12157412056981261No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1840.12157412056981261No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA1810.11959193382139176No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA1680.11100245791156804No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1670.11034172899542775No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA1600.10571662658244575No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1580.10439516875016518No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG1570.1037344398340249No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC1560.10307371091788461No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA1520.10043079525332346No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTGTG200.001836889437.07
CTAGTAC303.5848527E-430.8333343
TAGTTAC250.005481457329.635
GTTACAG250.005481457329.637
ACTGTGC250.005481457329.68
TCCCCGC250.005481457329.68
GCCTGCC358.833697E-426.4285716
CATAGCC509.02315E-625.90000233
ATGGGGC400.001923488223.1255
TCTTATA2150.020.65116337
GAATGTA450.003810701720.5555551
CAATGCT450.003810701720.5555554
CTTATAC1001.2676537E-820.3537
AGAGGGG555.1147793E-420.1818188
TACTGGT701.2103416E-418.52
CCCTTAT500.00700737118.51
TTTTGCG500.00700737118.52
ATACACT609.1863784E-418.54
CGCCTTA852.6979002E-517.41176425
TATGCCC650.001571377617.07692336
Produced by FastQC (version 0.11.5)