FastQCFastQC Report
Fri 10 Feb 2017
ERR1630170.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630170.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128866
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18661.448015768317477No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18411.4286157714214767No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12660.9824158428134653No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7870.6107119022860956No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3470.2692719569164869No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2570.1994319680908851No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA1950.15131997578880388No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1700.13191997889280338No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1630.12648797976192325No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1600.12415998013440319No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA1530.11872798100352304No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT1520.11795198112768301No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA1480.11484798162432294No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTT1480.11484798162432294No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1440.11174398212096286No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1390.10786398274176276No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATAT200.001835878237.02
AGCTTTT200.001835878237.07
TAGCTTT200.001835878237.06
TCTCTGC303.5819237E-430.8333328
CATGCTG250.005478474329.59999834
TGCATCC250.005478474329.59999812
CGCCGGA250.005478474329.59999814
GCTTTTC250.005478474329.5999988
TTCTCTG250.005478474329.5999987
CATCCTA358.8265457E-426.42857214
ACTAAAA400.001921946423.12522
ACGGTAG450.003807675320.5555575
TCAGTAC450.003807675320.5555573
GCGCGCC555.1091117E-420.18181811
CGCGCCT555.1091117E-420.18181812
GCGCCTT555.1091117E-420.18181813
GTATCGC555.1091117E-420.1818186
TCGCGCG555.1091117E-420.1818189
CGCGCGC656.841541E-519.92307710
TGTATCG609.176285E-418.55
Produced by FastQC (version 0.11.5)