FastQCFastQC Report
Fri 10 Feb 2017
ERR1630169.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630169.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569763
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT51510.9040601092033004No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT31980.5612860083929634No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT30800.5405756428550117No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13010.22834055563453576No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTAGCG251.2328444E-437.015
ACCTTAT250.00549404629.61
TTAGACT454.003592E-628.7777794
AGCGGAA358.8638806E-426.42857218
ATTAGAC509.080584E-625.9000023
AGCTAGT509.080584E-625.9000026
AAGTACG451.3220678E-424.66666835
CGTTAAA451.3220678E-424.66666817
TTCGGTC1150.024.13043628
CCTACAT1002.0008883E-1124.0500011
TACATAC1002.0008883E-1124.0500013
TACACTT701.9193794E-723.7857155
GCTAGTC551.9004756E-523.5454547
GCGGAAT400.001929998423.12519
CTCTATA400.001929998423.1252
AAGGAGC502.699785E-422.26
GTACGGG502.699785E-422.237
TCCGGGT502.699785E-422.213
TCGGTCA1250.022.19999929
GCAGTCG1900.021.4210539
Produced by FastQC (version 0.11.5)