##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630169.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	569763
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.61699513657433	31.0	31.0	34.0	30.0	34.0
2	31.833235924410676	33.0	31.0	34.0	30.0	34.0
3	31.939711774895876	33.0	31.0	34.0	30.0	34.0
4	35.635476505143366	37.0	35.0	37.0	33.0	37.0
5	35.592658701951514	37.0	35.0	37.0	33.0	37.0
6	35.67045245128237	37.0	35.0	37.0	33.0	37.0
7	35.66316521079818	37.0	35.0	37.0	33.0	37.0
8	35.66266500281696	37.0	35.0	37.0	33.0	37.0
9	37.37367642335497	39.0	37.0	39.0	34.0	39.0
10	37.24036134322517	39.0	37.0	39.0	34.0	39.0
11	37.322560433022154	39.0	37.0	39.0	34.0	39.0
12	37.23328296151207	39.0	37.0	39.0	34.0	39.0
13	37.26957348932802	39.0	37.0	39.0	34.0	39.0
14	38.3794384682754	40.0	38.0	41.0	34.0	41.0
15	38.37065762431046	40.0	38.0	41.0	34.0	41.0
16	38.32389256585633	40.0	38.0	41.0	34.0	41.0
17	38.325168534987355	40.0	38.0	41.0	34.0	41.0
18	38.324817511842646	40.0	38.0	41.0	34.0	41.0
19	38.37270759947557	40.0	38.0	41.0	34.0	41.0
20	38.35939504671241	40.0	38.0	41.0	34.0	41.0
21	38.31438334886611	40.0	38.0	41.0	34.0	41.0
22	38.276488645278825	40.0	38.0	41.0	34.0	41.0
23	38.22722254691863	40.0	38.0	41.0	34.0	41.0
24	38.18856787822305	40.0	38.0	41.0	33.0	41.0
25	38.17465858611388	40.0	38.0	41.0	33.0	41.0
26	37.986561078904735	40.0	37.0	41.0	33.0	41.0
27	37.83473479323859	40.0	37.0	41.0	33.0	41.0
28	37.808392261343755	40.0	37.0	41.0	33.0	41.0
29	37.73843861395001	40.0	37.0	41.0	33.0	41.0
30	37.635336095885485	40.0	37.0	41.0	33.0	41.0
31	37.53701977839909	40.0	37.0	41.0	32.0	41.0
32	37.42668969378496	39.0	36.0	41.0	32.0	41.0
33	37.31183492083551	39.0	36.0	41.0	31.0	41.0
34	37.28908335571106	39.0	36.0	41.0	31.0	41.0
35	37.12583653203174	39.0	36.0	41.0	31.0	41.0
36	37.0538487055144	39.0	36.0	41.0	31.0	41.0
37	36.98081483002582	39.0	35.0	41.0	31.0	41.0
38	36.880648620566795	39.0	35.0	41.0	31.0	41.0
39	36.77210875399069	39.0	35.0	41.0	30.0	41.0
40	36.6478553363416	39.0	35.0	41.0	30.0	41.0
41	36.504248257608864	39.0	35.0	40.0	30.0	41.0
42	36.448958602085426	39.0	35.0	40.0	30.0	41.0
43	35.2101944141687	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	5.0
12	3.0
13	1.0
14	1.0
15	0.0
16	3.0
17	4.0
18	15.0
19	35.0
20	91.0
21	150.0
22	299.0
23	594.0
24	970.0
25	1610.0
26	2528.0
27	3689.0
28	5245.0
29	7363.0
30	9977.0
31	12758.0
32	16588.0
33	21435.0
34	28711.0
35	37407.0
36	52067.0
37	81453.0
38	138417.0
39	148344.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.059307115414654	20.039209285264224	12.99540335192001	23.906080247401114
2	19.137255314929188	21.77150850441324	35.29485066597866	23.79638551467891
3	19.088112074669645	25.75193545386415	29.69796213513338	25.461990336332825
4	13.233572555606454	16.067031379714024	38.14357899688116	32.555817067798365
5	12.710899093131706	39.1504888874848	34.98015841674521	13.15845360263829
6	30.927596211056173	38.42036074648582	16.43437710065413	14.217665941803872
7	28.627868078481754	30.84510577204908	22.43722389835774	18.08980225111143
8	28.848661636505003	32.95703652220309	19.507584732599344	18.68671710869256
9	27.744167311671696	13.071224351177596	20.198222769818326	38.986385567332384
10	18.169308993388476	27.07423963999066	32.80083122280668	21.955620143814183
11	34.739356539473434	23.764091385365493	20.247190498505518	21.249361576655556
12	21.48524912990138	28.99696891514542	27.950568920761793	21.56721303419141
13	31.614899528400404	19.6300918101035	24.756784838608333	23.998223822887763
14	23.70775217063937	20.46517587137108	26.148767118959988	29.67830483902956
15	25.855311769981554	27.704677207891702	22.576229063663313	23.86378195846343
16	24.286589336267888	25.893397781182703	24.660253473812794	25.159759408736615
17	21.830831415869405	27.612182609260344	26.13367312373741	24.423312851132838
18	22.665564453992275	25.426010463999944	27.19183239346886	24.716592688538917
19	23.507317955009363	25.45514538501096	28.858490284556915	22.179046375422764
20	24.89842267749924	25.38897752223293	27.981458957496365	21.731140842771467
21	24.745376586405225	25.460059709036916	26.1984368939366	23.59612681062126
22	25.300519689765743	24.676751561614214	27.382437961046964	22.64029078757308
23	24.11897578466836	25.64680402202319	26.94681823846055	23.28740195484789
24	22.4661833077964	26.173163227517403	27.213946851585657	24.146706613100534
25	23.944341770174617	25.651367322904434	27.658342152789846	22.745948754131103
26	23.466774781795237	25.92411230634492	27.12689311169732	23.482219800162525
27	22.570612693347936	26.59210935073004	27.160415822017224	23.676862133904798
28	22.904611215540495	25.59660771232951	28.785828493601727	22.712952578528263
29	22.903207122961653	25.769837634244414	29.028560998169418	22.298394244624518
30	22.85511695213624	27.638860368258385	26.83396429743595	22.67205838216943
31	23.945219328036394	27.073011058984175	27.045455742124357	21.936313870855077
32	22.3422721377134	27.133738063019187	27.459487541311034	23.06450225795638
33	22.390186796966457	26.565431591732004	27.548296396922932	23.49608521437861
34	23.756368876181853	24.5825018472593	28.2670162857188	23.394112990840053
35	22.610980354989707	26.436079562905977	28.033936917630665	22.91900316447365
36	22.84563932722904	27.355760202048923	26.045390802842583	23.753209667879453
37	22.66960122015645	26.178955109405138	27.712048693930637	23.439394976507778
38	22.66100115311103	26.704787780182286	27.483708138296098	23.150502928410585
39	21.541588344627502	26.397115993843055	28.72755865157969	23.33373700994975
40	21.94631803047934	24.863495874600492	29.811167099302693	23.379018995617475
41	21.450497838574986	25.25593975038744	29.36080440463842	23.93275800639915
42	20.564515421324305	25.62346098289991	29.701121343435776	24.110902252340008
43	21.26252494458222	24.58373042826579	30.167104568039694	23.986640059112297
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	88.0
1	80.5
2	73.0
3	208.5
4	344.0
5	344.0
6	510.5
7	677.0
8	610.0
9	543.0
10	768.0
11	993.0
12	993.0
13	2057.5
14	3122.0
15	5907.5
16	8693.0
17	7181.0
18	5669.0
19	5669.0
20	5876.5
21	6084.0
22	4414.0
23	2744.0
24	2979.0
25	3214.0
26	3214.0
27	4089.5
28	4965.0
29	6655.0
30	8345.0
31	10052.0
32	11759.0
33	11759.0
34	15113.5
35	18468.0
36	22238.5
37	26009.0
38	29425.5
39	32842.0
40	32842.0
41	37556.5
42	42271.0
43	45642.0
44	49013.0
45	50545.0
46	52077.0
47	52077.0
48	53604.5
49	55132.0
50	53077.0
51	51022.0
52	47620.0
53	44218.0
54	44218.0
55	41821.0
56	39424.0
57	35170.0
58	30916.0
59	28179.5
60	25443.0
61	25443.0
62	22152.0
63	18861.0
64	15318.0
65	11775.0
66	9238.5
67	6702.0
68	6702.0
69	4866.0
70	3030.0
71	2357.5
72	1685.0
73	1438.0
74	1191.0
75	1191.0
76	1101.0
77	1011.0
78	967.5
79	924.0
80	598.0
81	272.0
82	272.0
83	173.5
84	75.0
85	60.5
86	46.0
87	33.0
88	20.0
89	20.0
90	15.5
91	11.0
92	9.0
93	7.0
94	5.0
95	3.0
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	569763.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.361341529255675
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.38283009162525	24.815109985993065
2	11.439688847530192	7.63286733261744
3	4.056928511287825	4.060337328745437
4	2.0319861640639756	2.711591376082418
5	1.297398996277081	2.1641485507256606
6	0.9012382348370911	1.8039909930974236
7	0.6453208000769934	1.507013732511077
8	0.5079303935658295	1.3556191466271108
9	0.4417063798332894	1.326232565394255
>10	3.55552290426061	24.76086332063332
>50	0.46532448865819703	10.71515628973277
>100	0.27148329985278713	14.813910613946746
>500	5.281776261727376E-4	0.09004184943353577
>1k	0.0015845328785182129	1.335473927312657
>5k	5.281776261727376E-4	0.9076429871470506
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5151	0.9040601092033004	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3198	0.5612860083929634	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3080	0.5405756428550117	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1301	0.22834055563453576	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.7551157235552327E-4	0.0
4	0.0	0.0	0.0	1.7551157235552327E-4	0.0
5	0.0	0.0	0.0	1.7551157235552327E-4	0.0
6	0.0	0.0	0.0	1.7551157235552327E-4	0.0
7	0.0	0.0	0.0	3.5102314471104653E-4	0.0
8	0.0	0.0	0.0	3.5102314471104653E-4	0.0
9	0.0	0.0	0.0	5.265347170665698E-4	0.0
10	0.0	0.0	0.0	7.020462894220931E-4	0.0
11	0.0	0.0	0.0	7.020462894220931E-4	0.0
12	0.0	0.0	0.0	7.020462894220931E-4	0.0
13	0.0	0.0	0.0	8.775578617776163E-4	0.0
14	0.0	0.0	0.0	8.775578617776163E-4	0.0
15	0.0	0.0	0.0	8.775578617776163E-4	0.0
16	0.0	0.0	0.0	0.0015796041511997093	0.0
17	0.0	0.0	0.0	0.0015796041511997093	0.0
18	0.0	0.0	0.0	0.0017551157235552327	0.0
19	0.0	0.0	0.0	0.0017551157235552327	0.0
20	0.0	0.0	0.0	0.002106138868266279	0.0
21	0.0	0.0	0.0	0.0031592083023994186	0.0
22	0.0	0.0	0.0	0.005089835598310175	0.0
23	0.0	0.0	0.0	0.008951090190131687	0.0
24	0.0	0.0	0.0	0.012285810064886628	0.0
25	0.0	0.0	0.0	0.014742972077863953	0.0
26	0.0	0.0	0.0	0.01667359937377471	0.0
27	0.0	0.0	0.0	0.021763434972084883	0.0
28	0.0	0.0	0.0	0.043877893088880814	0.0
29	0.0	0.0	0.0	0.08968641347367239	0.0
30	0.0	0.0	0.0	0.17375645663196804	0.0
31	0.0	0.0	0.0	0.44632592850009567	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAGCG	25	1.2328444E-4	37.0	15
ACCTTAT	25	0.005494046	29.6	1
TTAGACT	45	4.003592E-6	28.777779	4
AGCGGAA	35	8.8638806E-4	26.428572	18
ATTAGAC	50	9.080584E-6	25.900002	3
AGCTAGT	50	9.080584E-6	25.900002	6
AAGTACG	45	1.3220678E-4	24.666668	35
CGTTAAA	45	1.3220678E-4	24.666668	17
TTCGGTC	115	0.0	24.130436	28
CCTACAT	100	2.0008883E-11	24.050001	1
TACATAC	100	2.0008883E-11	24.050001	3
TACACTT	70	1.9193794E-7	23.785715	5
GCTAGTC	55	1.9004756E-5	23.545454	7
GCGGAAT	40	0.0019299984	23.125	19
CTCTATA	40	0.0019299984	23.125	2
AAGGAGC	50	2.699785E-4	22.2	6
GTACGGG	50	2.699785E-4	22.2	37
TCCGGGT	50	2.699785E-4	22.2	13
TCGGTCA	125	0.0	22.199999	29
GCAGTCG	190	0.0	21.421053	9
>>END_MODULE