FastQCFastQC Report
Fri 10 Feb 2017
ERR1630168.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630168.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2207873
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT123120.5576407700986424No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT95660.4332676743635164No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT85140.38562000622318404No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49990.22641700858699754No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC43790.19833568325714387No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT32810.14860456194717722No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAC702.0736479E-1029.07142828
TATACTG2450.017.3673465
CAGGACA15800.017.0949364
TCCGCGA1301.394983E-817.07692326
CCAGGAC17100.016.6608183
CTTATAC15150.016.60726237
GACAGGC14850.016.5690237
TCTAGCG3050.016.37704828
CTAGCGG3050.016.37704829
TGCATCA14150.016.34275614
GCTGCAT14100.016.26950312
GCATCAG14650.015.911262515
CAGCGTT957.064386E-515.5789489
ACAGGCT16100.015.2826098
CGGTCCA3400.015.23529310
TCCAGGA18750.015.0960012
TCTTATA25450.014.90176837
ATCAAGC15050.014.87375430
TAGTGCG750.00410599814.825
GTTATAC1252.9615494E-614.7999993
Produced by FastQC (version 0.11.5)