##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630168.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2207873
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.73189128179021	31.0	31.0	34.0	30.0	34.0
2	31.942112612455517	33.0	31.0	34.0	30.0	34.0
3	32.05586145579932	33.0	31.0	34.0	30.0	34.0
4	35.72293107438698	37.0	35.0	37.0	33.0	37.0
5	35.65234458684897	37.0	35.0	37.0	33.0	37.0
6	35.70830342143773	37.0	35.0	37.0	33.0	37.0
7	35.6863012501172	37.0	35.0	37.0	33.0	37.0
8	35.69683084126669	37.0	35.0	37.0	33.0	37.0
9	37.416081450337046	39.0	37.0	39.0	34.0	39.0
10	37.30211157978742	39.0	37.0	39.0	34.0	39.0
11	37.37516288301003	39.0	37.0	39.0	34.0	39.0
12	37.30216185441826	39.0	37.0	39.0	34.0	39.0
13	37.3087677597398	39.0	37.0	39.0	34.0	39.0
14	38.50570526475028	40.0	38.0	41.0	34.0	41.0
15	38.497343370746414	40.0	38.0	41.0	34.0	41.0
16	38.45681839489862	40.0	38.0	41.0	34.0	41.0
17	38.440049767355276	40.0	38.0	41.0	34.0	41.0
18	38.376018457583385	40.0	38.0	41.0	34.0	41.0
19	38.41162557810164	40.0	38.0	41.0	34.0	41.0
20	38.394267695650974	40.0	38.0	41.0	34.0	41.0
21	38.33784461334506	40.0	38.0	41.0	34.0	41.0
22	38.2852600670419	40.0	38.0	41.0	34.0	41.0
23	38.21997053272539	40.0	38.0	41.0	33.0	41.0
24	38.18052487620438	40.0	38.0	41.0	33.0	41.0
25	38.15603524296914	40.0	38.0	41.0	33.0	41.0
26	37.95698620346369	40.0	37.0	41.0	33.0	41.0
27	37.830903770280266	40.0	37.0	41.0	33.0	41.0
28	37.73277946693492	40.0	37.0	41.0	33.0	41.0
29	37.650364853413215	40.0	37.0	41.0	32.0	41.0
30	37.570077626747555	40.0	37.0	41.0	32.0	41.0
31	37.50132412507422	40.0	37.0	41.0	32.0	41.0
32	37.391666549661146	40.0	36.0	41.0	32.0	41.0
33	37.27515441331997	39.0	36.0	41.0	31.0	41.0
34	37.192261058493855	39.0	36.0	41.0	31.0	41.0
35	37.03463514432216	39.0	36.0	41.0	31.0	41.0
36	36.93334942725419	39.0	35.0	41.0	31.0	41.0
37	36.845704440427504	39.0	35.0	41.0	30.0	41.0
38	36.72006406165572	39.0	35.0	41.0	30.0	41.0
39	36.62026348435802	39.0	35.0	41.0	30.0	41.0
40	36.47598888160687	39.0	35.0	41.0	30.0	41.0
41	36.32489685774499	39.0	35.0	40.0	30.0	41.0
42	36.25338730986791	39.0	35.0	40.0	29.0	41.0
43	35.00957346731447	38.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	8.0
11	12.0
12	14.0
13	10.0
14	4.0
15	9.0
16	30.0
17	59.0
18	105.0
19	288.0
20	553.0
21	974.0
22	1627.0
23	2907.0
24	4689.0
25	7147.0
26	10889.0
27	15856.0
28	21684.0
29	29667.0
30	38914.0
31	50534.0
32	64033.0
33	81457.0
34	107952.0
35	142093.0
36	194863.0
37	296277.0
38	518337.0
39	616877.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.38287256558688	19.12437898375495	12.66331894995772	25.829429500700446
2	18.477421482123294	21.26209252072017	34.672420016912206	25.588065980244334
3	19.83003551381805	23.517204114548253	29.608904135337493	27.0438562362962
4	14.59626527431605	15.736502960088735	34.40125405763828	35.265977707956935
5	14.756374121156426	36.86638678945754	33.26649675955093	15.110742329835094
6	34.21324505530889	35.080505083399274	15.918623942590902	14.78762591870094
7	29.201724918054616	30.520369604592297	21.558984597393057	18.71892087996003
8	27.84132058320383	33.299469670583406	19.801274801585055	19.057934944627704
9	27.318373837625625	14.017925849901694	19.789906394072485	38.873793918400196
10	17.676922540381625	26.637084651155206	31.977201587228976	23.708791221234193
11	35.49434229233294	22.3459410935321	21.20330290736831	20.95641370676665
12	22.08075373900582	25.79681892934965	28.95918379363306	23.16324353801147
13	29.954847946417207	19.4671070301598	25.188994113338946	25.38905091008405
14	22.808558282111335	20.714325506947183	25.66374968125431	30.81336652968717
15	25.462107648401876	27.357234768485323	22.50337768522012	24.677279897892678
16	24.454395701202017	26.05140784818692	24.22734459817209	25.266851852438975
17	23.355736493901595	26.633189499577192	25.90103688029157	24.110037126229635
18	23.26954494212303	24.786932944059735	27.18507812722924	24.758443986588
19	24.047714700981444	25.155522985244172	27.119087012704078	23.677675301070305
20	24.056773193023332	25.167072562597575	26.860014140306077	23.916140104073015
21	25.739161627503034	24.833448300694833	26.080259145340335	23.347130926461805
22	25.078389925507487	25.106335373456716	26.291186132535703	23.52408856850009
23	24.114521079790368	25.329763079669892	26.244625483440398	24.311090357099342
24	24.105643757589316	25.641828130512938	26.367594512909033	23.88493359898871
25	24.28717593810876	24.96579286942682	26.62621446070494	24.120816731759483
26	24.31874478287474	25.731733664028685	26.592788625070373	23.356732928026204
27	24.62356304008428	25.393670741025414	25.9817480443848	24.00101817450551
28	23.65068099478548	25.242710971147343	27.24173899495125	23.864869039115927
29	23.487220505889606	25.394078373167297	27.389301830313613	23.729399290629487
30	23.308813505124615	26.017302625649215	26.967402563462663	23.70648130576351
31	23.755533040170334	25.802797534097294	26.46506388727975	23.97660553845262
32	23.14114081742926	25.59476926435533	26.77830654208824	24.48578337612716
33	23.38857352755344	25.560301702135945	26.852722054212357	24.198402716098254
34	24.12176787342388	25.463285252367324	26.698908859341092	23.716038014867703
35	24.109312446866284	25.435520974258935	27.219047472386322	23.236119106488463
36	23.54777652518963	25.839122087185267	26.17813615185294	24.434965235772165
37	23.345364520513634	25.015569283197	27.194453666492592	24.44461252979678
38	23.409543936630413	24.938617393301154	27.606932101619975	24.044906568448457
39	22.73282928864115	24.909222586625226	27.852734283176613	24.505213841557012
40	23.122933248425067	24.45924199444443	28.69087125935233	23.726953497778176
41	22.066939538641943	24.712789186696877	28.952254047221015	24.268017227440165
42	22.29100133929805	25.05837065809492	28.465314807509305	24.185313195097724
43	21.980566817022538	23.805264161480302	29.185193170078172	25.028975851418988
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	658.0
1	589.5
2	521.0
3	1242.5
4	1964.0
5	1964.0
6	2738.0
7	3512.0
8	3263.0
9	3014.0
10	4139.0
11	5264.0
12	5264.0
13	9552.0
14	13840.0
15	20599.5
16	27359.0
17	24337.5
18	21316.0
19	21316.0
20	24040.5
21	26765.0
22	23992.5
23	21220.0
24	24845.5
25	28471.0
26	28471.0
27	32420.5
28	36370.0
29	42267.5
30	48165.0
31	54748.5
32	61332.0
33	61332.0
34	71018.5
35	80705.0
36	90280.0
37	99855.0
38	106754.5
39	113654.0
40	113654.0
41	120505.5
42	127357.0
43	131146.5
44	134936.0
45	138326.5
46	141717.0
47	141717.0
48	143779.5
49	145842.0
50	147097.0
51	148352.0
52	151359.5
53	154367.0
54	154367.0
55	151407.0
56	148447.0
57	140923.5
58	133400.0
59	123136.0
60	112872.0
61	112872.0
62	105568.5
63	98265.0
64	86152.5
65	74040.0
66	62931.0
67	51822.0
68	51822.0
69	44625.5
70	37429.0
71	33605.5
72	29782.0
73	29547.5
74	29313.0
75	29313.0
76	26590.5
77	23868.0
78	18606.0
79	13344.0
80	8785.0
81	4226.0
82	4226.0
83	2953.0
84	1680.0
85	1444.0
86	1208.0
87	1031.0
88	854.0
89	854.0
90	733.5
91	613.0
92	357.0
93	101.0
94	72.0
95	43.0
96	43.0
97	24.5
98	6.0
99	5.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2207873.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.6264558472205
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.94707070007314	39.674897742117956
2	11.691890073234621	11.604541329798671
3	3.472660039199829	5.1700743032327185
4	1.4482887730644323	2.8749375540202875
5	0.7716314658714382	1.914666743569748
6	0.4853355249536983	1.4451289200121373
7	0.3159779271103206	1.0976605253905607
8	0.23110026375742346	0.917494962851103
9	0.18295227808511624	0.8171345835485485
>10	1.187533960571423	11.42628281712805
>50	0.1440656950835803	5.013495275414166
>100	0.10805755753832008	10.737311550015633
>500	0.009962556590409424	3.347766174483139
>1k	0.0031989860611406394	2.580075542115922
>5k	1.8279920349375083E-4	0.8200795647385088
>10k+	9.139960174687542E-5	0.558452411563082
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	12312	0.5576407700986424	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9566	0.4332676743635164	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8514	0.38562000622318404	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4999	0.22641700858699754	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	4379	0.19833568325714387	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	3281	0.14860456194717722	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	4.5292460209441396E-5	0.0	4.5292460209441396E-5	0.0
6	0.0	4.5292460209441396E-5	0.0	4.5292460209441396E-5	4.5292460209441396E-5
7	0.0	4.5292460209441396E-5	0.0	4.5292460209441396E-5	4.5292460209441396E-5
8	0.0	4.5292460209441396E-5	0.0	4.5292460209441396E-5	4.5292460209441396E-5
9	1.3587738062832417E-4	4.5292460209441396E-5	0.0	9.058492041888279E-5	4.5292460209441396E-5
10	1.3587738062832417E-4	4.5292460209441396E-5	0.0	9.058492041888279E-5	9.058492041888279E-5
11	1.3587738062832417E-4	4.5292460209441396E-5	0.0	1.3587738062832417E-4	9.058492041888279E-5
12	1.3587738062832417E-4	4.5292460209441396E-5	0.0	2.7175476125664835E-4	9.058492041888279E-5
13	1.3587738062832417E-4	4.5292460209441396E-5	0.0	3.1704722146608976E-4	9.058492041888279E-5
14	1.3587738062832417E-4	4.5292460209441396E-5	0.0	3.6233968167553117E-4	9.058492041888279E-5
15	1.3587738062832417E-4	4.5292460209441396E-5	0.0	5.888019827227382E-4	9.058492041888279E-5
16	1.3587738062832417E-4	4.5292460209441396E-5	0.0	0.0013134813460738005	1.3587738062832417E-4
17	1.3587738062832417E-4	4.5292460209441396E-5	0.0	0.0018116984083776557	1.3587738062832417E-4
18	1.3587738062832417E-4	4.5292460209441396E-5	0.0	0.0020834531696343043	1.3587738062832417E-4
19	1.3587738062832417E-4	4.5292460209441396E-5	0.0	0.002400500391100394	1.3587738062832417E-4
20	1.3587738062832417E-4	4.5292460209441396E-5	0.0	0.003215764674870339	1.8116984083776558E-4
21	1.3587738062832417E-4	4.5292460209441396E-5	0.0	0.0038498591178025184	2.2646230104720697E-4
22	1.3587738062832417E-4	4.5292460209441396E-5	0.0	0.005797434906808499	2.2646230104720697E-4
23	1.3587738062832417E-4	9.058492041888279E-5	0.0	0.008696152360212747	2.2646230104720697E-4
24	1.3587738062832417E-4	1.3587738062832417E-4	0.0	0.012863058699481357	2.2646230104720697E-4
25	1.3587738062832417E-4	1.3587738062832417E-4	0.0	0.014448294806811805	2.2646230104720697E-4
26	1.3587738062832417E-4	1.3587738062832417E-4	0.0	0.020019267412573098	2.2646230104720697E-4
27	1.3587738062832417E-4	1.3587738062832417E-4	0.0	0.03437697729896602	2.2646230104720697E-4
28	1.3587738062832417E-4	1.3587738062832417E-4	0.0	0.08188876805867004	2.2646230104720697E-4
29	1.3587738062832417E-4	1.3587738062832417E-4	0.0	0.1515485718607909	2.2646230104720697E-4
30	1.3587738062832417E-4	1.3587738062832417E-4	0.0	0.25042201249800145	2.2646230104720697E-4
31	1.3587738062832417E-4	1.3587738062832417E-4	0.0	0.5357192193572728	2.2646230104720697E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGAAC	70	2.0736479E-10	29.071428	28
TATACTG	245	0.0	17.367346	5
CAGGACA	1580	0.0	17.094936	4
TCCGCGA	130	1.394983E-8	17.076923	26
CCAGGAC	1710	0.0	16.660818	3
CTTATAC	1515	0.0	16.607262	37
GACAGGC	1485	0.0	16.569023	7
TCTAGCG	305	0.0	16.377048	28
CTAGCGG	305	0.0	16.377048	29
TGCATCA	1415	0.0	16.342756	14
GCTGCAT	1410	0.0	16.269503	12
GCATCAG	1465	0.0	15.9112625	15
CAGCGTT	95	7.064386E-5	15.578948	9
ACAGGCT	1610	0.0	15.282609	8
CGGTCCA	340	0.0	15.235293	10
TCCAGGA	1875	0.0	15.096001	2
TCTTATA	2545	0.0	14.901768	37
ATCAAGC	1505	0.0	14.873754	30
TAGTGCG	75	0.004105998	14.8	25
GTTATAC	125	2.9615494E-6	14.799999	3
>>END_MODULE