FastQCFastQC Report
Fri 10 Feb 2017
ERR1630167.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630167.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1798859
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT49430.2747852944560969No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG48640.27039362173466625No Hit
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG47910.2663354937768886No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT44240.24593367240011585No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT44210.24576690001828935No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC43850.2437656314363716No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG42670.23720591775119676No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT36270.2016278096282143No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA34450.1915102851307412No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG32100.17844644855433361No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA31930.17750140505731687No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC31810.17683431553001097No Hit
GGTTCAGACAGCAGCTCTGCCAAGAAGTACTTGGCCAGTTCCT31110.17294295995405978No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC30900.17177555328127442No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA30770.17105287296002633No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC30330.1686068780265713No Hit
GTCCATCGTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCG28790.16004589575947864No Hit
GTCCTGGCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCA28690.15948998782005705No Hit
CTTCTTGGCAGAGCTGCTGTCTGAACCCAACCAGACGGAGAAT28420.15798903638361872No Hit
ATTTCATCCTGCTCAGCAGCCTGGGACAGATCTTCAGGTTCCA28070.15604335859564314No Hit
CCCTCGGACCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGG26930.14970600808623688No Hit
GCCAAGAAGTACTTGGCCAGTTCCTGCTTCCCCGCGGCAGCAG26740.14864978300133586No Hit
GTTCCTGCTTCCCCGCGGCAGCAGCCAGGGACTTCTGCAGAAA26300.1462037880678808No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC24330.13525240166127528No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23510.13069395655801816No Hit
GGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTC22690.12613551145476104No Hit
CTCCAGCTCGGCTTTCGCGGCGCCGAGATGCTGTCCTGCCGCC22630.12580196669110807No Hit
GTTCGGAGCCATCGCTGCTGCCTGCTGATCCGCGCCTAGAGTT22050.12257770064246282No Hit
GAGTTTGACCAGCCACTCTCCAGCTCGGCTTTCGCGGCGCCGA21440.11918666221199105No Hit
CCCCAGACTCCGTCAGTTTCTGCAGAAGTCCCTGGCTGCTGCC20990.11668507648459384No Hit
GGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGCCAGCTTTG20470.11379435519960153No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC20300.11284931170258482No Hit
GTACATGGGAAGCCGCTTTAGGAGCGAGGTTCGGAGCCATCGC20260.11262694852681616No Hit
AGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAAGAAAT20170.11212663138133673No Hit
ATGCTGTCCTGCCGCCTCCAGTGCGCGCTGGCTGCGCTGTCCA19580.10884677453874929No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC19460.10817968501144336No Hit
CGCCTAGAGTTTGACCAGCCACTCTCCAGCTCGGCTTTCGCGG19230.1069010967507737No Hit
GCCAGGGACTTCTGCAGAAACTGACGGAGTCTGGGGTCCGAGG19220.10684550595683152No Hit
TAGCAGATCTCTGCAGCTCAAGCCTCATTTCATCCTGCTCAGC18520.10295415038088032No Hit
ATACTAGTTAAGAAAGCTAACAGGATGTGAAAGTCTTCCAGAA18090.10056374624136744No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATAC1002.8771683E-718.53
CGAATTA609.239584E-418.515
GTACTAA701.2196838E-418.51
GGTATCA22400.017.343751
TTTAGCG650.001580403717.07692326
TTAGCGG650.001580403717.07692327
GTTTAGG2003.6379788E-1215.7251
GTTCTAC957.063221E-515.5789481
ATTTGCG2150.015.48837216
CGGACCA3000.015.4166679
TGCGTTA855.3662434E-415.23529437
TTGCGCG2201.8189894E-1215.13636418
ACGGACC2950.015.0508478
GTACTAG1152.2111059E-514.4782611
TCTTTCG2201.8189894E-1114.29545410
TTTAGAC1304.447036E-614.2307693
CTTATAC8500.013.92941137
CTATACA1601.7848834E-713.8750014
GTTAACG800.00630044513.87500113
TATACTG1601.7848834E-713.8750015
Produced by FastQC (version 0.11.5)