FastQCFastQC Report
Fri 10 Feb 2017
ERR1630166.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630166.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences419021
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT56731.3538700924297349No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT48711.162471570637271No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT36640.8744191818548473No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17890.42694757541984774No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA7400.17660212733967987No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA6500.15512349023079988No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6490.15488483870736788No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5820.1388951866374239No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5230.12481474675493591No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG5150.12290553456747991No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA5110.12195092847375191No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4930.11765520105197591No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4860.11598464038795192No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT4810.11479138277079191No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC4640.11073430687244792No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC4570.1090637462084239No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC4520.10787048859126393No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA4510.10763183706783191No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4440.10596127640380792No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA4400.10500667031007993No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA4280.10214285202889593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCAT401.5968562E-632.37524
AACGTGA352.3847137E-531.7142856
ATTAGAC303.5956095E-430.8333343
TTAGGTT250.00549240629.64
AGACTAT250.00549240629.66
TAGGTAT250.00549240629.631
GCTTGGG250.00549240629.618
AGTTGTG250.00549240629.635
ACCACCA250.00549240629.619
TATGTAC250.00549240629.610
CTGTGCG250.00549240629.69
TACGTAG250.00549240629.614
TCTCGTC454.0002687E-628.77777712
TAAACGT405.9312595E-527.7500024
ATCAATC604.309004E-827.75000236
CGTGCGC358.8599534E-426.4285710
GCGTTTT358.8599534E-426.4285713
ATATAAC358.8599534E-426.428573
ACGTGCG358.8599534E-426.428579
ACATATA358.8599534E-426.428571
Produced by FastQC (version 0.11.5)