##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630166.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	419021
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.718453251746332	31.0	31.0	34.0	30.0	34.0
2	31.88017545660003	33.0	31.0	34.0	30.0	34.0
3	31.99222234685135	33.0	31.0	34.0	30.0	34.0
4	35.67064657857244	37.0	35.0	37.0	33.0	37.0
5	35.61705976550101	37.0	35.0	37.0	33.0	37.0
6	35.7111147174008	37.0	35.0	37.0	33.0	37.0
7	35.708312948515704	37.0	35.0	37.0	33.0	37.0
8	35.71323155641364	37.0	35.0	37.0	33.0	37.0
9	37.35794148741949	39.0	37.0	39.0	34.0	39.0
10	37.27462585407414	39.0	37.0	39.0	34.0	39.0
11	37.35871949138588	39.0	37.0	39.0	34.0	39.0
12	37.23997842590228	39.0	37.0	39.0	34.0	39.0
13	37.30092763847158	39.0	37.0	39.0	34.0	39.0
14	38.387815885122706	40.0	38.0	41.0	34.0	41.0
15	38.38927165941564	40.0	38.0	41.0	34.0	41.0
16	38.32388591502574	40.0	38.0	41.0	34.0	41.0
17	38.3714396175848	40.0	38.0	41.0	34.0	41.0
18	38.42055648762234	40.0	38.0	41.0	34.0	41.0
19	38.4363385128669	40.0	38.0	41.0	34.0	41.0
20	38.41279554008033	40.0	38.0	41.0	34.0	41.0
21	38.37091458423325	40.0	38.0	41.0	34.0	41.0
22	38.34763651463769	40.0	38.0	41.0	34.0	41.0
23	38.29729297576971	40.0	38.0	41.0	34.0	41.0
24	38.275795246538955	40.0	38.0	41.0	34.0	41.0
25	38.24366797845454	40.0	38.0	41.0	34.0	41.0
26	38.00174454263629	40.0	37.0	41.0	33.0	41.0
27	37.87013300049401	40.0	37.0	41.0	33.0	41.0
28	37.78773140248341	40.0	37.0	41.0	33.0	41.0
29	37.70206027860179	40.0	37.0	41.0	33.0	41.0
30	37.552886370850146	39.0	36.0	41.0	33.0	41.0
31	37.34190171852962	39.0	36.0	41.0	32.0	41.0
32	37.19427427264982	39.0	35.0	41.0	32.0	41.0
33	37.10243162037225	39.0	35.0	41.0	31.0	41.0
34	36.973061016035	39.0	35.0	41.0	31.0	41.0
35	36.83200126008004	39.0	35.0	41.0	31.0	41.0
36	36.65868775073326	39.0	35.0	41.0	31.0	41.0
37	36.52564668596562	39.0	35.0	40.0	30.0	41.0
38	36.35843310955776	39.0	35.0	40.0	30.0	41.0
39	36.20078468620905	39.0	35.0	40.0	30.0	41.0
40	35.99996658878672	38.0	35.0	40.0	29.0	41.0
41	35.7641645645445	38.0	35.0	40.0	28.0	41.0
42	35.601194212223255	38.0	35.0	40.0	27.0	41.0
43	34.46879512005365	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	4.0
12	3.0
13	0.0
14	0.0
15	1.0
16	4.0
17	4.0
18	13.0
19	26.0
20	56.0
21	147.0
22	312.0
23	495.0
24	809.0
25	1267.0
26	2042.0
27	3048.0
28	4324.0
29	5940.0
30	7774.0
31	9909.0
32	12896.0
33	16447.0
34	21763.0
35	28744.0
36	39599.0
37	62503.0
38	95057.0
39	105832.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.727402683875034	20.02930640707745	13.452547724338398	22.790743184709118
2	18.848697320659348	22.876896384668072	34.14363480589278	24.130771488779796
3	20.095890182114978	25.789160925108767	30.42592137386909	23.689027518907167
4	13.76064684108911	17.639927354476267	36.13613637502655	32.46328942940807
5	12.842554430446206	39.9326048097828	33.77587280828408	13.448967951486917
6	29.533364676233408	36.6399297409915	17.636347581624786	16.1903580011503
7	27.23658241472384	32.411740700346755	21.894368062698526	18.457308822230868
8	28.672071328167327	32.70766858940244	19.91356041821293	18.706699664217307
9	27.47571124120271	13.637025351951335	20.975798349008763	37.9114650578372
10	20.25459344519726	27.485973256710285	30.13906224270383	22.12037105538863
11	33.69306072965317	24.53194469966899	20.25459344519726	21.520401125480586
12	20.642163519250825	29.072528584486218	27.990482577245533	22.294825319017423
13	31.40510857451059	20.16891754828517	25.498722021092025	22.927251856112225
14	22.678099665649214	22.4594948701855	27.238252975387866	27.624152488777415
15	27.328940554292032	27.352089752064934	23.321742824345318	21.99722686929772
16	22.38002391288265	26.35261717193172	26.91989184312958	24.34746707205605
17	20.85862045100365	28.71240343562733	27.166180215311403	23.262795898057615
18	20.95169454514213	25.650743041518204	28.978022581207146	24.41953983213252
19	21.551425823526746	27.40053601132163	30.18345142606218	20.86458673908945
20	22.43014073280337	25.244796800160373	31.1822080516251	21.14285441541116
21	22.934650053338615	25.94500036990986	29.113815298039952	22.006534278711566
22	23.237021533526956	26.355003687166032	28.282592041926303	22.125382737380704
23	22.623448466783287	26.83373864317063	28.77230496800876	21.77050792203732
24	21.21087009958928	27.472847422921525	28.536755914381377	22.779526563107815
25	22.49362203803628	27.30770056870658	29.23314105975596	20.965536333501188
26	22.10080163046721	27.462108104367083	28.91239341226335	21.524696852902363
27	21.302750936110602	27.869486254865507	28.87707298679541	21.95068982222848
28	20.9388073628768	28.36993849950241	29.72166072822126	20.96959340939953
29	21.598917476689714	28.40836139477496	28.743905436720357	21.24881569181497
30	20.970070712446393	29.264404409325547	29.511170084554234	20.254354793673826
31	22.099369721326617	27.65732505053446	28.862992546912924	21.380312681226002
32	19.971075435360042	28.638898766410275	28.918598351872575	22.471427446357104
33	20.43071826949007	28.307889103410094	29.821178413492405	21.440214213607433
34	20.542168530932816	27.616992943074454	29.342920760534675	22.497917765458055
35	21.410144121655	28.944611367926665	29.015490870385975	20.629753640032362
36	20.968877454829233	28.883277926404645	28.20049591786569	21.94734870090043
37	21.16218518880915	28.59164576477074	28.589736552583283	21.656432493836824
38	20.692041687648114	28.433419804735323	29.1298049501099	21.744733557506667
39	20.441934891091375	27.673076051080976	29.845998171929335	22.038990885898322
40	20.354111130468404	28.107183172203783	30.203020850983602	21.335684846344215
41	20.01641922481212	27.147088093436842	30.138584939656965	22.697907742094074
42	19.192355514401427	27.867815694201486	30.177246486452947	22.762582304944143
43	19.715241002240937	27.401251965891927	30.290844611606577	22.59266242026056
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	73.0
1	76.0
2	79.0
3	284.0
4	489.0
5	489.0
6	691.0
7	893.0
8	888.0
9	883.0
10	1269.5
11	1656.0
12	1656.0
13	3296.0
14	4936.0
15	8024.0
16	11112.0
17	9472.5
18	7833.0
19	7833.0
20	8710.0
21	9587.0
22	6854.5
23	4122.0
24	4239.0
25	4356.0
26	4356.0
27	4857.0
28	5358.0
29	6012.5
30	6667.0
31	7832.0
32	8997.0
33	8997.0
34	11212.5
35	13428.0
36	15229.0
37	17030.0
38	18902.0
39	20774.0
40	20774.0
41	24248.0
42	27722.0
43	30440.5
44	33159.0
45	36643.0
46	40127.0
47	40127.0
48	43155.0
49	46183.0
50	42213.0
51	38243.0
52	34767.0
53	31291.0
54	31291.0
55	29422.0
56	27553.0
57	23658.0
58	19763.0
59	16319.5
60	12876.0
61	12876.0
62	10814.0
63	8752.0
64	6935.5
65	5119.0
66	4238.0
67	3357.0
68	3357.0
69	2925.5
70	2494.0
71	1984.5
72	1475.0
73	1236.5
74	998.0
75	998.0
76	818.5
77	639.0
78	522.0
79	405.0
80	309.0
81	213.0
82	213.0
83	193.0
84	173.0
85	134.0
86	95.0
87	90.5
88	86.0
89	86.0
90	54.0
91	22.0
92	12.5
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	419021.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.02078579121325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.09525214827195	19.361032596365956
2	11.658396395862601	6.53354856155037
3	4.771031629532441	4.010641658826948
4	2.7092522822902962	3.0366151142564797
5	1.8592225431637668	2.604843831009332
6	1.3203176850590315	2.2197803417633803
7	1.0502265243297113	2.059972072934528
8	0.8449403157295895	1.8940713274735133
9	0.666070144619266	1.6797427959868994
>10	5.2110158802073245	29.370418914443285
>50	0.566414759279424	10.921184679738008
>100	0.23849042495975745	11.49494127341554
>500	0.005962260623993936	0.9952433426103605
>1k	0.0025552545531402583	2.464002942232461
>5k	8.517515177134195E-4	1.353960547392944
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5673	1.3538700924297349	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4871	1.162471570637271	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3664	0.8744191818548473	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1789	0.42694757541984774	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	740	0.17660212733967987	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	650	0.15512349023079988	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	649	0.15488483870736788	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	582	0.1388951866374239	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	523	0.12481474675493591	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	515	0.12290553456747991	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	511	0.12195092847375191	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	493	0.11765520105197591	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	486	0.11598464038795192	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT	481	0.11479138277079191	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	464	0.11073430687244792	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	457	0.1090637462084239	No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC	452	0.10787048859126393	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	451	0.10763183706783191	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	444	0.10596127640380792	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	440	0.10500667031007993	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	428	0.10214285202889593	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	2.3865152343199983E-4	0.0	0.0	0.0
11	0.0	2.3865152343199983E-4	0.0	2.3865152343199983E-4	0.0
12	0.0	2.3865152343199983E-4	0.0	9.546060937279993E-4	0.0
13	0.0	2.3865152343199983E-4	0.0	9.546060937279993E-4	0.0
14	0.0	2.3865152343199983E-4	0.0	0.0016705606640239987	0.0
15	0.0	2.3865152343199983E-4	0.0	0.0023865152343199984	0.0
16	0.0	2.3865152343199983E-4	0.0	0.005727636562367996	0.0
17	0.0	2.3865152343199983E-4	0.0	0.009068757890415993	0.0
18	0.0	2.3865152343199983E-4	0.0	0.011216621601303992	0.0
19	0.0	2.3865152343199983E-4	0.0	0.01479639445278399	0.0
20	0.0	2.3865152343199983E-4	0.0	0.01575100054651199	0.0
21	0.0	2.3865152343199983E-4	0.0	0.02410380386663198	0.0
22	0.0	2.3865152343199983E-4	0.0	0.03412716785077598	0.0
23	0.0	2.3865152343199983E-4	0.0	0.05321928972533596	0.0
24	0.0	2.3865152343199983E-4	0.0	0.07398197226391995	0.0
25	0.0	2.3865152343199983E-4	0.0	0.08114151796687995	0.0
26	0.0	2.3865152343199983E-4	0.0	0.09021027585729594	0.0
27	0.0	2.3865152343199983E-4	0.0	0.10858644316155992	0.0
28	0.0	2.3865152343199983E-4	0.0	0.1369859744499679	0.0
29	0.0	2.3865152343199983E-4	0.0	0.18376167304263988	0.0
30	0.0	2.3865152343199983E-4	0.0	0.2718240851890478	0.0
31	0.0	2.3865152343199983E-4	0.0	0.5049866235821117	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTCAT	40	1.5968562E-6	32.375	24
AACGTGA	35	2.3847137E-5	31.714285	6
ATTAGAC	30	3.5956095E-4	30.833334	3
TTAGGTT	25	0.005492406	29.6	4
AGACTAT	25	0.005492406	29.6	6
TAGGTAT	25	0.005492406	29.6	31
GCTTGGG	25	0.005492406	29.6	18
AGTTGTG	25	0.005492406	29.6	35
ACCACCA	25	0.005492406	29.6	19
TATGTAC	25	0.005492406	29.6	10
CTGTGCG	25	0.005492406	29.6	9
TACGTAG	25	0.005492406	29.6	14
TCTCGTC	45	4.0002687E-6	28.777777	12
TAAACGT	40	5.9312595E-5	27.750002	4
ATCAATC	60	4.309004E-8	27.750002	36
CGTGCGC	35	8.8599534E-4	26.42857	10
GCGTTTT	35	8.8599534E-4	26.42857	13
ATATAAC	35	8.8599534E-4	26.42857	3
ACGTGCG	35	8.8599534E-4	26.42857	9
ACATATA	35	8.8599534E-4	26.42857	1
>>END_MODULE