FastQCFastQC Report
Fri 10 Feb 2017
ERR1630164.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630164.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1651639
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC93580.566588703705834No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT56910.3445668212000322No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT35440.21457473455155757No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG35100.21251617332843317No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG34990.21185016822683408No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG34040.2060983059857511No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT32750.19828788252154375No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31900.1931414794637327No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT31420.19023527538402762No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG30500.18466505089792623No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT30460.18442286722461748No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT27870.16874147437787557No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC26870.16268688254515665No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG26430.16002286213876035No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG26320.15935685703716126No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC24900.15075933663470042No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG24550.14864022949324884No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG24520.14845859173826725No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT24400.14773204071834098No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT23960.14506802031194468No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23440.14191963255893086No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT21240.12859953052694928No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG20850.12623823971218892No Hit
GCTCACACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACG19130.11582434175991244No Hit
TGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGA18030.10916429074392166No Hit
GAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGA17970.1088010152339585No Hit
CTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCA17880.10825610196901381No Hit
GGTCCCCAGAGGGCCAGCAGCGCCAGCAGGGGCAGGAGGCGCA17590.10650027033752532No Hit
GCCCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGCTGGCCC17570.10637917850087095No Hit
CACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACGAGGCT17550.10625808666421659No Hit
TTGTGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCT16750.10141441319804147No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTA656.904464E-519.92307715
TCTAGCG1601.8189894E-1218.528
ATCCGTA701.2196069E-418.512
TCCGTAT701.2196069E-418.513
CAGGACA22800.017.4451754
TACGTAG852.7242817E-517.41176414
CGAATTA752.0679552E-417.26666615
TGCATCA21150.017.14420914
GACAGGC21500.016.8651167
GCTGCAT21450.016.73193612
GCATCAG21800.016.7178915
GGTATCA14600.016.345891
CTGCATC22350.016.30648813
CCAGGAC25800.016.133723
ACAGGCT23100.015.9372298
TCCAGGA24900.015.8995982
CTAGCGG1902.7284841E-1115.57894829
ATCAGAA23450.015.54157817
GGACAGG24800.015.3669356
ATCAAGC23150.015.34341230
Produced by FastQC (version 0.11.5)