##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630164.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1651639
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.838626963882543	33.0	31.0	34.0	30.0	34.0
2	32.05786252322693	33.0	31.0	34.0	30.0	34.0
3	32.16913805014292	34.0	31.0	34.0	30.0	34.0
4	35.81631518751979	37.0	35.0	37.0	35.0	37.0
5	35.744473822669484	37.0	35.0	37.0	35.0	37.0
6	35.78926569304794	37.0	35.0	37.0	35.0	37.0
7	35.767796110409115	37.0	35.0	37.0	35.0	37.0
8	35.76101799485239	37.0	35.0	37.0	34.0	37.0
9	37.51684902088168	39.0	37.0	39.0	35.0	39.0
10	37.40606149406741	39.0	37.0	39.0	34.0	39.0
11	37.469254479943864	39.0	37.0	39.0	35.0	39.0
12	37.39571056387019	39.0	37.0	39.0	34.0	39.0
13	37.39054296974096	39.0	37.0	39.0	34.0	39.0
14	38.63737172590378	40.0	38.0	41.0	34.0	41.0
15	38.64873861661053	40.0	38.0	41.0	34.0	41.0
16	38.6073264193931	40.0	38.0	41.0	34.0	41.0
17	38.567299512786995	40.0	38.0	41.0	34.0	41.0
18	38.45338236745439	40.0	38.0	41.0	34.0	41.0
19	38.48201998136397	40.0	38.0	41.0	34.0	41.0
20	38.45324492821979	40.0	38.0	41.0	34.0	41.0
21	38.40783609493358	40.0	38.0	41.0	34.0	41.0
22	38.35587619328437	40.0	38.0	41.0	34.0	41.0
23	38.29815776934306	40.0	38.0	41.0	34.0	41.0
24	38.24777811616219	40.0	38.0	41.0	33.0	41.0
25	38.22528772934037	40.0	38.0	41.0	33.0	41.0
26	38.02444480906542	40.0	37.0	41.0	33.0	41.0
27	37.90674414929655	40.0	37.0	41.0	33.0	41.0
28	37.81142065548222	40.0	37.0	41.0	33.0	41.0
29	37.74577132169923	40.0	37.0	41.0	32.0	41.0
30	37.68508614775989	40.0	37.0	41.0	32.0	41.0
31	37.63961737401454	40.0	37.0	41.0	32.0	41.0
32	37.55777382345658	40.0	37.0	41.0	32.0	41.0
33	37.448087021437495	40.0	37.0	41.0	32.0	41.0
34	37.365533267257554	40.0	36.0	41.0	31.0	41.0
35	37.20202296022315	40.0	36.0	41.0	31.0	41.0
36	37.11249673808865	39.0	36.0	41.0	31.0	41.0
37	37.02663717676805	39.0	36.0	41.0	31.0	41.0
38	36.91825453382973	39.0	36.0	41.0	30.0	41.0
39	36.845266429286305	39.0	35.0	41.0	30.0	41.0
40	36.702074121524134	39.0	35.0	41.0	30.0	41.0
41	36.5546096937648	39.0	35.0	41.0	30.0	41.0
42	36.52081114577701	39.0	35.0	41.0	30.0	41.0
43	35.21400560291928	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	4.0
11	2.0
12	1.0
13	2.0
14	1.0
15	8.0
16	8.0
17	38.0
18	88.0
19	182.0
20	335.0
21	685.0
22	1228.0
23	2124.0
24	3317.0
25	5313.0
26	7848.0
27	10950.0
28	15219.0
29	20308.0
30	27452.0
31	35701.0
32	45470.0
33	58408.0
34	78730.0
35	102765.0
36	137959.0
37	206382.0
38	379162.0
39	511945.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.36359216511598	17.239420963055487	11.420534390384338	27.97645248144419
2	19.83345028786557	19.082499262853446	33.700524146014956	27.383526303266027
3	20.971471368743412	21.1640679349422	28.32949573121003	29.53496496510436
4	15.702402280401467	14.293680398682762	31.926407647191667	38.0775096737241
5	16.18719344844727	35.39798951223603	32.219389345976936	16.195427693339767
6	37.64061032707511	32.053130254250476	14.82527356159548	15.480985857078938
7	31.719037876921046	28.65384021568878	19.791310328709848	19.835811578680328
8	28.948577746105535	32.27981417246747	18.579604865227815	20.19200321619918
9	28.097544318098567	13.295157113630761	17.777916360657507	40.829382207613165
10	18.832020798733865	25.42286783007667	30.7337741479827	25.011337223206766
11	37.785557255550394	20.454227588474236	19.948911354115516	21.81130380185985
12	24.018081433049232	23.355890724304768	27.405443925700474	25.220583916945532
13	31.14276182628286	17.93721267177634	23.700094269994835	27.219931231945964
14	23.69500841285535	19.08921985978776	23.859935494378615	33.35583623297827
15	27.221626517659125	25.415299590285773	20.574471782272035	26.78860210978307
16	26.29975436520935	24.31560407570904	21.662784664203254	27.72185689487836
17	25.248798314886002	25.107363049673687	24.06682089730262	25.57701773813769
18	25.32968766177113	22.885327847065852	24.965867238543048	26.81911725261997
19	26.265364283599503	23.174555698914837	24.07711370341824	26.48296631406742
20	25.700047044178543	24.084803035045795	24.097093856466216	26.118056064309453
21	28.20997808843216	23.01507775004102	23.31447731616897	25.460466845357853
22	27.271758538034035	23.732365244463228	23.534501183369976	25.46137503413276
23	26.31973451825732	23.89147991782708	23.371148295723216	26.417637268192383
24	26.80410186487483	23.69028583122583	23.676602453683888	25.82900985021545
25	26.478607007947865	23.250601372333783	24.17919412171788	26.091597498000468
26	27.187115344212625	23.686531984289545	24.148921162554288	24.97743150894354
27	27.562560583759527	23.36485152021719	23.030214229622818	26.042373666400465
28	25.74079444721274	22.84639682158147	24.857368952900725	26.555439778305068
29	25.79801034003193	23.047469816346066	25.006735733413898	26.1477841102081
30	25.46155667188774	23.659165229205655	24.775874146832326	26.103403952074274
31	25.539115993264872	23.659770688388928	24.399157443000558	26.40195587534564
32	25.184861825132487	23.43399495894684	24.626689004074134	26.754454211846536
33	26.277594559101598	23.600132958836646	24.14413803500644	25.978134447055318
34	26.68525022719856	24.024075478963624	23.817129530121292	25.473544763716525
35	26.966364925991698	23.471170152799733	24.581945570430342	24.980519350778227
36	26.395719645757943	24.12718517787483	22.94072736233523	26.536367814032
37	25.461253942296107	23.604734448629515	24.434274075630327	26.49973753344405
38	26.54587352320937	23.29655572434412	24.68002995812039	25.47754079432612
39	24.875653820235534	23.55738754049765	24.93262752938142	26.634331109885395
40	25.78983664105776	22.575151107475666	25.984612860316332	25.65039939115025
41	24.413506825644102	22.923229591938675	26.388756865150313	26.27450671726691
42	24.856158034534182	23.61593544352004	25.12970449353642	26.398202028409358
43	24.786954049886205	21.51644517960644	26.737259171041615	26.95934159946574
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	239.0
1	198.0
2	157.0
3	373.0
4	589.0
5	589.0
6	782.0
7	975.0
8	879.0
9	783.0
10	1121.5
11	1460.0
12	1460.0
13	2662.5
14	3865.0
15	5678.5
16	7492.0
17	6963.0
18	6434.0
19	6434.0
20	8133.0
21	9832.0
22	9700.0
23	9568.0
24	11472.5
25	13377.0
26	13377.0
27	15755.0
28	18133.0
29	21224.0
30	24315.0
31	28665.0
32	33015.0
33	33015.0
34	39388.5
35	45762.0
36	52119.0
37	58476.0
38	63153.0
39	67830.0
40	67830.0
41	73510.0
42	79190.0
43	82462.5
44	85735.0
45	88548.5
46	91362.0
47	91362.0
48	93769.5
49	96177.0
50	102982.5
51	109788.0
52	118917.5
53	128047.0
54	128047.0
55	131143.5
56	134240.0
57	127831.5
58	121423.0
59	110554.0
60	99685.0
61	99685.0
62	97784.5
63	95884.0
64	84715.0
65	73546.0
66	62578.5
67	51611.0
68	51611.0
69	45100.0
70	38589.0
71	35966.5
72	33344.0
73	37771.5
74	42199.0
75	42199.0
76	40747.5
77	39296.0
78	29760.5
79	20225.0
80	12551.5
81	4878.0
82	4878.0
83	3326.5
84	1775.0
85	1440.5
86	1106.0
87	886.5
88	667.0
89	667.0
90	555.5
91	444.0
92	257.5
93	71.0
94	58.0
95	45.0
96	45.0
97	26.0
98	7.0
99	5.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1651639.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.51950559614554
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.47889238551306	35.38360994332299
2	11.538971934834349	10.274186512532482
3	3.5316067217045966	4.71676155630939
4	1.5790404644283846	2.811924031706389
5	0.8696034405209594	1.9357157618350134
6	0.5233239470830766	1.39788740344572
7	0.3690843535128614	1.1502017059165914
8	0.26387407194130547	0.939803457797493
9	0.23015308038981364	0.9221671215346
>10	1.309866105078492	11.401307782451948
>50	0.15955189554003377	4.9239341522870355
>100	0.12010084615360804	10.518470859409245
>500	0.015967884926519942	4.955518277063497
>1k	0.009689913075067657	7.754146616443363
>5k	2.729552978892298E-4	0.9143648179441953
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	9358	0.566588703705834	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	5691	0.3445668212000322	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	3544	0.21457473455155757	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	3510	0.21251617332843317	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	3499	0.21185016822683408	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	3404	0.2060983059857511	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3275	0.19828788252154375	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3190	0.1931414794637327	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	3142	0.19023527538402762	No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG	3050	0.18466505089792623	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	3046	0.18442286722461748	No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT	2787	0.16874147437787557	No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC	2687	0.16268688254515665	No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG	2643	0.16002286213876035	No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG	2632	0.15935685703716126	No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC	2490	0.15075933663470042	No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG	2455	0.14864022949324884	No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG	2452	0.14845859173826725	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	2440	0.14773204071834098	No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT	2396	0.14506802031194468	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2344	0.14191963255893086	No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT	2124	0.12859953052694928	No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG	2085	0.12623823971218892	No Hit
GCTCACACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACG	1913	0.11582434175991244	No Hit
TGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGA	1803	0.10916429074392166	No Hit
GAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGA	1797	0.1088010152339585	No Hit
CTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCA	1788	0.10825610196901381	No Hit
GGTCCCCAGAGGGCCAGCAGCGCCAGCAGGGGCAGGAGGCGCA	1759	0.10650027033752532	No Hit
GCCCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGCTGGCCC	1757	0.10637917850087095	No Hit
CACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACGAGGCT	1755	0.10625808666421659	No Hit
TTGTGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCT	1675	0.10141441319804147	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	6.0545918327188934E-5	0.0	0.0	3.632755099631336E-4	0.0
3	6.0545918327188934E-5	0.0	0.0	6.054591832718893E-4	0.0
4	6.0545918327188934E-5	0.0	0.0	8.476428565806451E-4	0.0
5	6.0545918327188934E-5	0.0	0.0	9.68734693235023E-4	0.0
6	6.0545918327188934E-5	0.0	0.0	0.0010898265298894007	0.0
7	6.0545918327188934E-5	0.0	0.0	0.0012714642848709677	0.0
8	6.0545918327188934E-5	0.0	0.0	0.0013320102031981564	6.0545918327188934E-5
9	6.0545918327188934E-5	0.0	0.0	0.0015136479581797232	6.0545918327188934E-5
10	6.0545918327188934E-5	0.0	0.0	0.001816377549815668	6.0545918327188934E-5
11	1.2109183665437787E-4	0.0	0.0	0.001998015304797235	6.0545918327188934E-5
12	1.2109183665437787E-4	0.0	0.0	0.0022401989781059904	6.0545918327188934E-5
13	1.2109183665437787E-4	0.0	0.0	0.0022401989781059904	6.0545918327188934E-5
14	1.2109183665437787E-4	0.0	0.0	0.0023007448964331794	6.0545918327188934E-5
15	1.816377549815668E-4	0.0	0.0	0.0024823826514147464	6.0545918327188934E-5
16	1.816377549815668E-4	0.0	0.0	0.002724566324723502	6.0545918327188934E-5
17	1.816377549815668E-4	0.0	0.0	0.0032089336713410133	6.0545918327188934E-5
18	1.816377549815668E-4	0.0	0.0	0.0033300255079953913	1.2109183665437787E-4
19	1.816377549815668E-4	0.0	0.0	0.003632755099631336	1.2109183665437787E-4
20	1.816377549815668E-4	0.0	0.0	0.004238214282903225	1.2109183665437787E-4
21	1.816377549815668E-4	0.0	0.0	0.004904219384502304	1.2109183665437787E-4
22	1.816377549815668E-4	0.0	0.0	0.005751862241082949	1.2109183665437787E-4
23	1.816377549815668E-4	0.0	0.0	0.006781142852645161	1.2109183665437787E-4
24	1.816377549815668E-4	0.0	0.0	0.008355336729152072	1.2109183665437787E-4
25	1.816377549815668E-4	0.0	0.0	0.009990076523986174	1.2109183665437787E-4
26	1.816377549815668E-4	0.0	0.0	0.012593551012055297	1.2109183665437787E-4
27	1.816377549815668E-4	0.0	0.0	0.021130525496188938	1.2109183665437787E-4
28	1.816377549815668E-4	0.0	0.0	0.04674144894858986	1.2109183665437787E-4
29	1.816377549815668E-4	0.0	0.0	0.08076825504847003	1.2109183665437787E-4
30	2.4218367330875574E-4	0.0	0.0	0.12442186216237325	1.2109183665437787E-4
31	2.4218367330875574E-4	0.0	0.0	0.2559275967690276	1.2109183665437787E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATTA	65	6.904464E-5	19.923077	15
TCTAGCG	160	1.8189894E-12	18.5	28
ATCCGTA	70	1.2196069E-4	18.5	12
TCCGTAT	70	1.2196069E-4	18.5	13
CAGGACA	2280	0.0	17.445175	4
TACGTAG	85	2.7242817E-5	17.411764	14
CGAATTA	75	2.0679552E-4	17.266666	15
TGCATCA	2115	0.0	17.144209	14
GACAGGC	2150	0.0	16.865116	7
GCTGCAT	2145	0.0	16.731936	12
GCATCAG	2180	0.0	16.71789	15
GGTATCA	1460	0.0	16.34589	1
CTGCATC	2235	0.0	16.306488	13
CCAGGAC	2580	0.0	16.13372	3
ACAGGCT	2310	0.0	15.937229	8
TCCAGGA	2490	0.0	15.899598	2
CTAGCGG	190	2.7284841E-11	15.578948	29
ATCAGAA	2345	0.0	15.541578	17
GGACAGG	2480	0.0	15.366935	6
ATCAAGC	2315	0.0	15.343412	30
>>END_MODULE