FastQCFastQC Report
Fri 10 Feb 2017
ERR1630163.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630163.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146477
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21901.495115274070332No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17321.1824381984884997No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13220.9025307727493054No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7050.481304232063737No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA3830.26147449770271103No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA2590.17681956894256437No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2300.15702123882930424No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2230.1522423315605863No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2210.15087692948380974No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA1550.10581866095018332No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTTAA251.2283727E-437.023
GCTTAAA251.2283727E-437.024
GATGCAA251.2283727E-437.035
CGGTAAG251.2283727E-437.032
TCGGTAA200.00183669737.031
TGCAATT251.2283727E-437.037
ACATTAG303.584296E-430.83333217
CATTAGG303.584296E-430.83333218
CAGATGT303.584296E-430.8333324
AAACAGA303.584296E-430.83333230
GCTGTCC303.584296E-430.8333328
TGTGTAT303.584296E-430.8333328
AATACTG303.584296E-430.8333325
AACAGAT303.584296E-430.83333231
ACTGGTG303.584296E-430.8333328
AGATGCA303.584296E-430.83333234
CAGAACT250.005480889729.64
GCAGTCC250.005480889729.613
TATCTCT250.005480889729.629
CTGCCTA250.005480889729.69