FastQCFastQC Report
Fri 10 Feb 2017
ERR1630162.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630162.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1373341
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT39100.2847071484795109No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT35350.25740147567137367No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT26040.18961059197970498No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17820.12975655718426815No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAA400.001931377523.1250021
TTGGCCG2100.019.3809531
CTAGCGG1950.018.02564229
GGTATCA16850.018.0059341
AACTCGA953.6074216E-617.52631626
TGCGTTA852.7237422E-517.41176437
GTACTAG752.0676362E-417.2666661
TCTAGCG2050.017.14634328
TCGCCAT2400.016.95833413
TTACTCG803.383165E-416.187519
AATGCGT957.061274E-515.57894835
ACTCGAA957.061274E-515.57894827
GTGCGTG957.061274E-515.5789489
AAATGCG957.061274E-515.57894834
TCTATAC1201.9360705E-615.4166673
AGGTCGC2600.014.94230710
TAGACTT750.00410494814.85
AAGACGG2750.014.85
TGTCGTA750.00410494814.832
CAAGACG3400.014.6911754
Produced by FastQC (version 0.11.5)