##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630162.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1373341
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.683049584917367	31.0	31.0	34.0	30.0	34.0
2	31.903135492204775	33.0	31.0	34.0	30.0	34.0
3	32.01888897222176	33.0	31.0	34.0	30.0	34.0
4	35.69845071253243	37.0	35.0	37.0	33.0	37.0
5	35.603603183768634	37.0	35.0	37.0	33.0	37.0
6	35.655302652436646	37.0	35.0	37.0	33.0	37.0
7	35.63957312859661	37.0	35.0	37.0	33.0	37.0
8	35.639532352125215	37.0	35.0	37.0	33.0	37.0
9	37.35251914855815	39.0	37.0	39.0	34.0	39.0
10	37.22214584724406	39.0	37.0	39.0	34.0	39.0
11	37.298876972288745	39.0	37.0	39.0	34.0	39.0
12	37.226402619597025	39.0	37.0	39.0	34.0	39.0
13	37.22925405998947	39.0	37.0	39.0	34.0	39.0
14	38.43324272704303	40.0	38.0	41.0	34.0	41.0
15	38.43133060179518	40.0	38.0	41.0	34.0	41.0
16	38.37767677510538	40.0	38.0	41.0	34.0	41.0
17	38.34757136064532	40.0	38.0	41.0	34.0	41.0
18	38.29498646002704	40.0	38.0	41.0	33.0	41.0
19	38.32868966993631	40.0	38.0	41.0	34.0	41.0
20	38.30972278552814	40.0	38.0	41.0	34.0	41.0
21	38.239716137507	40.0	38.0	41.0	34.0	41.0
22	38.20139353590987	40.0	38.0	41.0	33.0	41.0
23	38.14682952012647	40.0	38.0	41.0	33.0	41.0
24	38.094764519518456	40.0	38.0	41.0	33.0	41.0
25	38.0752580750156	40.0	37.0	41.0	33.0	41.0
26	37.88651908011193	40.0	37.0	41.0	33.0	41.0
27	37.76290375078003	40.0	37.0	41.0	33.0	41.0
28	37.68084255840319	40.0	37.0	41.0	32.0	41.0
29	37.61449996759727	40.0	37.0	41.0	32.0	41.0
30	37.54015936318802	40.0	37.0	41.0	32.0	41.0
31	37.4803482893178	40.0	37.0	41.0	32.0	41.0
32	37.379117058327104	39.0	37.0	41.0	32.0	41.0
33	37.28498821487162	39.0	36.0	41.0	31.0	41.0
34	37.22093056276628	39.0	36.0	41.0	31.0	41.0
35	37.06703506266834	39.0	36.0	41.0	31.0	41.0
36	36.972973209130146	39.0	36.0	41.0	31.0	41.0
37	36.90801774650287	39.0	36.0	41.0	30.0	41.0
38	36.778379149825135	39.0	35.0	41.0	30.0	41.0
39	36.69025536993362	39.0	35.0	40.0	30.0	41.0
40	36.57649556810726	39.0	35.0	40.0	30.0	41.0
41	36.44415188944333	39.0	35.0	40.0	30.0	41.0
42	36.39035607325493	39.0	35.0	40.0	30.0	41.0
43	35.13241212488377	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	6.0
11	2.0
12	3.0
13	2.0
14	5.0
15	6.0
16	17.0
17	38.0
18	86.0
19	147.0
20	329.0
21	616.0
22	1109.0
23	1805.0
24	3058.0
25	4552.0
26	6879.0
27	9695.0
28	13497.0
29	18162.0
30	24245.0
31	31252.0
32	40200.0
33	50497.0
34	66861.0
35	88210.0
36	123559.0
37	188688.0
38	345463.0
39	354348.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.23910885934375	19.241907144693123	12.668594325808375	25.850389670154755
2	18.62174070387471	21.58604454392609	34.313619123000045	25.47859562919916
3	20.0125096389025	23.464237942361002	29.839275169094932	26.683977249641565
4	15.172488114750815	15.907192751108429	34.09058638750317	34.82973274663758
5	14.477758983384318	36.74214925499202	34.161217061166894	14.618874700456768
6	34.396264292699335	34.75036425767526	15.957944894967818	14.895426554657584
7	28.942484058948214	30.823371617100197	21.428909498806195	18.805234825145394
8	27.042446122266796	33.80733554157344	20.226294853208344	18.92392348295143
9	26.906354648991037	14.446230033181854	19.619817656357743	39.027597661469365
10	17.099758909112886	26.912762380210015	33.022388467248845	22.96509024342825
11	35.21135682980411	22.009901401035865	21.408084372344526	21.370657396815503
12	21.000610918919627	25.873908956333498	29.35046721826553	23.775012906481347
13	29.520854616588306	19.863602703188793	25.766069752523222	24.849472927699676
14	22.9460126800263	21.19342537650882	24.950176249016085	30.910385694448795
15	25.164034278449414	27.87770844968584	22.31965695337138	24.638600318493367
16	25.03544276330496	26.648589097682223	24.0851325344543	24.230835604558518
17	23.549286011267412	26.922665237548433	25.5102702096566	24.01777854152756
18	23.440354580544817	25.42704251893739	26.865723807852525	24.26687909266526
19	24.16035056114978	25.52781865538129	26.914073052504804	23.397757730964123
20	24.553188173949515	24.983598392533246	26.644220190032918	23.81899324348432
21	24.504474853659797	25.618255043721845	25.787477400004803	24.08979270261355
22	24.385058044578876	25.352115752751864	26.092572784181062	24.170253418488198
23	23.70984336737926	25.584905715332173	26.450240690403916	24.255010226884654
24	24.03700173518449	25.986553958558	26.35361501622685	23.622829290030662
25	24.490639979437006	25.269252137670108	26.151334592064167	24.088773290828716
26	24.216272579060846	26.219926442158208	26.08631068321706	23.477490295563886
27	24.700129101221037	25.32291688662903	25.602308530801892	24.374645481348043
28	23.8191388737393	25.634347186896772	26.471866783267956	24.074647156095974
29	23.574552860505875	25.839321770776525	26.65536090453864	23.93076446417896
30	23.35836474699292	26.68972964471315	26.491089976924886	23.460815631369048
31	24.187146528065497	25.965801647223813	26.13043665047501	23.716615174235677
32	23.41210231107933	25.810341350036154	26.325508377016344	24.452047961868175
33	23.28678747667185	25.806336518024292	26.549633339425533	24.35724266587832
34	24.15809329219764	25.584323194312265	26.314658923020573	23.94292459046952
35	24.253117033569957	25.55599810971929	26.872568429836434	23.318316426874315
36	23.511276514718485	26.066796229050176	26.425483547057873	23.99644370917347
37	24.514377710998215	25.068428016057194	26.378444974700383	24.03874929824421
38	23.734163619960373	25.272310373024613	26.950043725484058	24.043482281530952
39	23.956322573927377	25.270999700729824	26.68841897241836	24.084258752924438
40	23.272006005791717	25.227601884746758	27.45123024798648	24.049161861475046
41	22.812032845447707	25.41036785474256	27.796519582536312	23.981079717273424
42	23.757318830501674	24.948355870828877	27.439070121695924	23.85525517697353
43	23.178875457734094	24.643988637927507	27.618486595827257	24.55864930851114
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	206.0
1	200.5
2	195.0
3	478.0
4	761.0
5	761.0
6	1034.5
7	1308.0
8	1270.0
9	1232.0
10	1742.0
11	2252.0
12	2252.0
13	3816.0
14	5380.0
15	7602.5
16	9825.0
17	9476.0
18	9127.0
19	9127.0
20	11531.0
21	13935.0
22	14340.0
23	14745.0
24	16818.5
25	18892.0
26	18892.0
27	21331.5
28	23771.0
29	29065.0
30	34359.0
31	38058.5
32	41758.0
33	41758.0
34	45659.0
35	49560.0
36	54742.0
37	59924.0
38	65954.5
39	71985.0
40	71985.0
41	77325.5
42	82666.0
43	80700.0
44	78734.0
45	83806.5
46	88879.0
47	88879.0
48	92042.5
49	95206.0
50	98829.5
51	102453.0
52	108202.5
53	113952.0
54	113952.0
55	104698.0
56	95444.0
57	89938.0
58	84432.0
59	75315.5
60	66199.0
61	66199.0
62	61790.5
63	57382.0
64	51510.0
65	45638.0
66	40093.0
67	34548.0
68	34548.0
69	29161.0
70	23774.0
71	21254.5
72	18735.0
73	15078.0
74	11421.0
75	11421.0
76	8656.0
77	5891.0
78	4952.0
79	4013.0
80	2970.0
81	1927.0
82	1927.0
83	1409.0
84	891.0
85	806.5
86	722.0
87	674.5
88	627.0
89	627.0
90	549.5
91	472.0
92	275.5
93	79.0
94	55.0
95	31.0
96	31.0
97	18.0
98	5.0
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1373341.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.52116369880747
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.48100780696043	44.89562004168409
2	8.412284763157315	8.836459702535382
3	2.211302485068379	3.4842053941756825
4	1.0087669629718365	2.119264591847707
5	0.5644778633971363	1.4823517133917037
6	0.3602015217414714	1.1350921852766027
7	0.26205277089056517	0.9634321534368624
8	0.17729351270586788	0.7449329282849191
9	0.13821471057318338	0.6533277695637713
>10	1.067455166670796	11.472959013397812
>50	0.16487296151008055	6.107045978966472
>100	0.14259995584685914	14.16081000573799
>500	0.00863397275550636	2.908986291810103
>1k	8.355457505328737E-4	1.0355122298908164
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3910	0.2847071484795109	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3535	0.25740147567137367	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2604	0.18961059197970498	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1782	0.12975655718426815	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	7.281512748836596E-5	0.0	0.0	0.0
11	0.0	1.4563025497673192E-4	0.0	0.0	0.0
12	0.0	1.4563025497673192E-4	0.0	2.184453824650979E-4	0.0
13	0.0	1.4563025497673192E-4	0.0	2.184453824650979E-4	0.0
14	0.0	1.4563025497673192E-4	0.0	2.9126050995346384E-4	0.0
15	0.0	1.4563025497673192E-4	0.0	3.640756374418298E-4	0.0
16	0.0	1.4563025497673192E-4	0.0	5.825210199069277E-4	0.0
17	0.0	1.4563025497673192E-4	0.0	8.737815298603916E-4	0.0
18	0.0	1.4563025497673192E-4	0.0	9.465966573487575E-4	0.0
19	0.0	1.4563025497673192E-4	0.0	0.0011650420398138554	0.0
20	0.0	1.4563025497673192E-4	0.0	0.0013834874222789532	0.0
21	0.0	1.4563025497673192E-4	0.0	0.002257268952139345	0.0
22	0.0	1.4563025497673192E-4	0.0	0.0030582353545113706	0.0
23	0.0	1.4563025497673192E-4	0.0	0.0046601681592554215	0.0
24	0.0	1.4563025497673192E-4	0.0	0.006917437111394767	0.0
25	0.0	1.4563025497673192E-4	0.0	0.008228109406185354	0.0
26	0.0	1.4563025497673192E-4	0.0	0.01172323552562692	0.0
27	0.0	1.4563025497673192E-4	0.0	0.02184453824650979	0.0
28	0.0	1.4563025497673192E-4	0.0	0.04594634544515892	0.0
29	0.0	1.4563025497673192E-4	0.0	0.07965974947227236	0.0
30	0.0	1.4563025497673192E-4	0.0	0.12757210335961716	0.0
31	0.0	1.4563025497673192E-4	0.0	0.263445131252908	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGTAA	40	0.0019313775	23.125002	1
TTGGCCG	210	0.0	19.38095	31
CTAGCGG	195	0.0	18.025642	29
GGTATCA	1685	0.0	18.005934	1
AACTCGA	95	3.6074216E-6	17.526316	26
TGCGTTA	85	2.7237422E-5	17.411764	37
GTACTAG	75	2.0676362E-4	17.266666	1
TCTAGCG	205	0.0	17.146343	28
TCGCCAT	240	0.0	16.958334	13
TTACTCG	80	3.383165E-4	16.1875	19
AATGCGT	95	7.061274E-5	15.578948	35
ACTCGAA	95	7.061274E-5	15.578948	27
GTGCGTG	95	7.061274E-5	15.578948	9
AAATGCG	95	7.061274E-5	15.578948	34
TCTATAC	120	1.9360705E-6	15.416667	3
AGGTCGC	260	0.0	14.942307	10
TAGACTT	75	0.004104948	14.8	5
AAGACGG	275	0.0	14.8	5
TGTCGTA	75	0.004104948	14.8	32
CAAGACG	340	0.0	14.691175	4
>>END_MODULE