FastQCFastQC Report
Fri 10 Feb 2017
ERR1630161.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630161.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264202
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT45461.7206531366151658No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31811.2040029977063005No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25110.9504091566301541No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14690.5560139590162073No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5750.2176365054011703No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4540.1718382146993588No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT4270.1616187614022604No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA4100.155184290807791No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3500.13247439459201674No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAA3410.12906791015965055No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT2980.11279248453834566No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2720.10295152951151014No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9550.031.7696321
CGGATGA250.005488780329.59999832
CTTTAGG250.005488780329.5999982
GTTTAAG551.8954775E-523.5454541
ATGCAGT400.001927274623.1256
TCTATCG400.001927274623.12531
GTATCAA13900.021.8273372
CTATCGC450.003818130120.55555532
GTACTAT450.003818130120.5555551
ATAAATC450.003818130120.5555553
TACAGGG555.128698E-420.1818182
ACTATAT609.21117E-418.53
GTACACA609.21117E-418.519
TCTCGCT609.21117E-418.52
TACCACG500.00702090118.4999984
CGCCTAT500.00702090118.49999836
TAATTTC500.00702090118.49999833
CTTCTGG500.00702090118.4999981
CCACGTG500.00702090118.4999986
TATCGCC500.00702090118.49999833