FastQCFastQC Report
Fri 10 Feb 2017
ERR1630160.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630160.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1527458
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT41840.27391915195049554No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG41620.2724788504823046No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT37130.24308360688149855No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT30450.19935081684733721No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG28130.1841621831827782No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT26970.17656786635049868No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC25450.16661669257027034No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA25130.16452170861653806No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT21410.14016752015440032No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC20050.13126383835103814No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT19540.12792495767477732No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC18720.12255656129333833No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC18580.12164000581358046No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC18500.1211162598251474No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA18280.11967595835695646No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18190.11908674411996927No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC17720.11600973643792498No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC17000.11129602254202735No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC17000.11129602254202735No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA16850.11031399881371534No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG16850.11031399881371534No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA16110.10546934842070943No Hit
GATGAACACCATTCTTGATAATCTTGCCGCCAGGGACTTTATA16000.10474919768661396No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA15490.10141031701035315No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACGC358.870738E-426.4285733
GCTAGAC701.21953075E-418.53
GCTTAGG10750.018.2418611
CTTAGGA11500.017.8565222
TAGGACA11400.017.3640354
TACGCTA752.0678269E-417.2666669
CGTCCTA650.001580255717.07692333
TTAGGAC11900.016.9453773
CCGAATT904.447496E-516.44444514
GTATTGG1606.2937033E-1016.18751
ATACGCT803.3834766E-416.18758
TATACGC803.3834766E-416.18757
CGAATTA957.062105E-515.57894815
CTACAGT1557.212293E-915.51612954
CGTTATT855.365586E-415.2352942
GCCCGTA1954.1836756E-1115.17948618
TCGCCAT6700.014.63432913
AGGACAC15050.014.6279065
CTAACAC1152.2106755E-514.478263
GGTATCA21550.014.4222751
Produced by FastQC (version 0.11.5)