##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630160.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1527458
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.86913551796514	33.0	31.0	34.0	30.0	34.0
2	32.08930851126512	33.0	31.0	34.0	30.0	34.0
3	32.20940412109531	34.0	31.0	34.0	30.0	34.0
4	35.844592126264686	37.0	35.0	37.0	35.0	37.0
5	35.77309752543114	37.0	35.0	37.0	35.0	37.0
6	35.83166476590518	37.0	35.0	37.0	35.0	37.0
7	35.807271296493916	37.0	35.0	37.0	35.0	37.0
8	35.81633275677629	37.0	35.0	37.0	35.0	37.0
9	37.56193230844973	39.0	37.0	39.0	35.0	39.0
10	37.46277933664952	39.0	37.0	39.0	35.0	39.0
11	37.51960512171202	39.0	37.0	39.0	35.0	39.0
12	37.45501807578343	39.0	37.0	39.0	35.0	39.0
13	37.453319174733444	39.0	37.0	39.0	35.0	39.0
14	38.706763131948634	40.0	38.0	41.0	35.0	41.0
15	38.72676302719944	40.0	38.0	41.0	35.0	41.0
16	38.68206392581662	40.0	38.0	41.0	34.0	41.0
17	38.65635258056195	40.0	38.0	41.0	34.0	41.0
18	38.60359499246461	40.0	38.0	41.0	34.0	41.0
19	38.650705289441674	40.0	38.0	41.0	34.0	41.0
20	38.62901173060077	40.0	38.0	41.0	34.0	41.0
21	38.56937801235779	40.0	38.0	41.0	34.0	41.0
22	38.551442985666384	40.0	38.0	41.0	34.0	41.0
23	38.49909719285244	40.0	38.0	41.0	34.0	41.0
24	38.47038020030665	40.0	38.0	41.0	34.0	41.0
25	38.44693798454688	40.0	38.0	41.0	34.0	41.0
26	38.28750708693791	40.0	38.0	41.0	34.0	41.0
27	38.16957782145237	40.0	38.0	41.0	33.0	41.0
28	38.1105817639503	40.0	38.0	41.0	33.0	41.0
29	38.070583282813665	40.0	38.0	41.0	33.0	41.0
30	38.003799777146085	40.0	37.0	41.0	33.0	41.0
31	37.94021635946782	40.0	37.0	41.0	33.0	41.0
32	37.859256359258325	40.0	37.0	41.0	33.0	41.0
33	37.77969607020291	40.0	37.0	41.0	33.0	41.0
34	37.72539408612217	40.0	37.0	41.0	33.0	41.0
35	37.61000367931557	40.0	37.0	41.0	32.0	41.0
36	37.52373682287827	40.0	37.0	41.0	32.0	41.0
37	37.462027761156115	40.0	37.0	41.0	32.0	41.0
38	37.35141719117645	40.0	37.0	41.0	31.0	41.0
39	37.27001986306661	40.0	36.0	41.0	31.0	41.0
40	37.18459034552832	40.0	36.0	41.0	31.0	41.0
41	37.0588860708445	39.0	36.0	41.0	31.0	41.0
42	37.02771336429545	39.0	36.0	41.0	31.0	41.0
43	35.80778849565749	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	3.0
13	4.0
14	1.0
15	3.0
16	11.0
17	24.0
18	36.0
19	110.0
20	195.0
21	457.0
22	813.0
23	1292.0
24	2275.0
25	3762.0
26	5561.0
27	8298.0
28	12048.0
29	16892.0
30	22734.0
31	29588.0
32	38285.0
33	49394.0
34	65337.0
35	88147.0
36	122820.0
37	187031.0
38	347998.0
39	524335.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.504867564280005	19.043927885414853	12.569249039908135	25.881955510397013
2	17.90216163063076	21.755229931035746	35.05523556130512	25.287372877028368
3	19.486427777392244	23.366796337444303	30.976301803388374	26.17047408177508
4	14.870392508337382	15.656338832229757	34.573192847201035	34.900075812231826
5	14.094266421728127	37.31945493754984	34.40618334513944	14.180095295582596
6	34.215736210095464	35.08319050343774	16.03474530887265	14.666327977594145
7	28.44647774275954	31.325967718916004	21.340554044693864	18.887000493630595
8	26.49519659460358	34.83696442062564	20.18196245003136	18.48587653473942
9	26.25721951110931	14.884860991267846	19.58521936446043	39.27270013316242
10	16.456753639052597	27.911929493315036	33.25138890889308	22.37992795873929
11	34.87251367959053	22.358454373213537	21.857818676520075	20.911213270675855
12	20.724825167042233	26.202226182323834	29.324406955870476	23.748541694763457
13	29.44820741388634	20.012727027518924	25.73916926030045	24.799896298294293
14	22.361793253889797	21.242744481353988	24.96998280803793	31.425479456718286
15	24.86575735633975	28.350435822130628	22.7949966545725	23.988810166957126
16	24.70882996455549	27.256854198282376	23.834043227375155	24.20027260978698
17	23.588864636539924	27.329065676437587	25.32449337395856	23.75757631306393
18	22.503204670766724	26.107755499660218	27.06260990482226	24.326429924750794
19	24.19994526854421	26.31823591876176	26.912818552130403	22.56900026056363
20	24.21677060842262	25.475855964615725	27.22307258202844	23.084300844933217
21	23.605951849412556	26.62187765555583	25.8318723002531	23.940298194778514
22	23.955879637934398	25.798418025241936	26.01465965021624	24.231042686607424
23	23.126593333499187	26.61808049713969	26.22775879925995	24.027567370101174
24	24.602051251163697	26.372312692067474	26.08759127910555	22.93804477766328
25	24.402765902564916	25.67631974168848	26.30271994385443	23.618194411892176
26	24.320472314132367	26.005952373158543	25.89236496191712	23.78121035079197
27	24.1578491847239	25.646989966336225	25.930271077829964	24.26488977110991
28	23.211309247128234	26.1209146176196	26.72767434521931	23.940101790032852
29	23.490007581223182	26.731864313126774	26.222521339375614	23.555606766274426
30	23.050126419187958	27.558204546377052	26.053285916863178	23.33838311757181
31	24.00354052288181	26.348547717842326	26.28969176239216	23.35821999688371
32	22.97994445673793	26.645184352041102	26.31666468079646	24.058206510424508
33	23.277170305173694	26.073581073914966	26.53316817876498	24.116080442146366
34	23.469319614680074	26.09891728610541	26.456112050216767	23.975651048997744
35	23.893357460565202	26.14566161557306	26.330543949489936	23.6304369743718
36	23.28659773296549	26.60760557737103	26.395750325049853	23.71004636461363
37	24.517269869286096	25.16606021245756	26.22239040287851	24.094279515377835
38	23.59325100919305	26.00470847643601	27.067454555215264	23.334585959155667
39	23.583692644904147	25.660934703278258	27.20591990090726	23.549452750910334
40	23.41773063481942	24.887820156102492	27.838605054934405	23.855844154143682
41	22.544777008598597	25.876979923506898	27.88057020225761	23.697672865636896
42	23.407844929287744	24.91269809055306	27.48265418754558	24.19680279261361
43	22.63787285804258	25.147074420376864	27.7054426373753	24.50961008420526
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	125.0
1	140.5
2	156.0
3	426.0
4	696.0
5	696.0
6	970.0
7	1244.0
8	1212.0
9	1180.0
10	1788.5
11	2397.0
12	2397.0
13	3913.0
14	5429.0
15	8648.0
16	11867.0
17	11134.0
18	10401.0
19	10401.0
20	12146.0
21	13891.0
22	14894.0
23	15897.0
24	18059.5
25	20222.0
26	20222.0
27	22767.0
28	25312.0
29	33785.5
30	42259.0
31	43971.0
32	45683.0
33	45683.0
34	50733.5
35	55784.0
36	59732.0
37	63680.0
38	75341.0
39	87002.0
40	87002.0
41	95129.0
42	103256.0
43	96645.5
44	90035.0
45	97503.0
46	104971.0
47	104971.0
48	110972.0
49	116973.0
50	120566.0
51	124159.0
52	129503.0
53	134847.0
54	134847.0
55	116252.5
56	97658.0
57	90695.0
58	83732.0
59	75526.0
60	67320.0
61	67320.0
62	61972.5
63	56625.0
64	50586.5
65	44548.0
66	38729.0
67	32910.0
68	32910.0
69	28377.0
70	23844.0
71	20775.5
72	17707.0
73	14315.0
74	10923.0
75	10923.0
76	8636.5
77	6350.0
78	5129.5
79	3909.0
80	2988.5
81	2068.0
82	2068.0
83	1561.5
84	1055.0
85	839.0
86	623.0
87	516.0
88	409.0
89	409.0
90	328.5
91	248.0
92	146.5
93	45.0
94	30.5
95	16.0
96	16.0
97	8.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1527458.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.31069231415991
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.22734205810113	41.872251984104665
2	9.838801294658905	9.899938093514848
3	2.7923957344041566	4.214620878389404
4	1.201069671110336	2.4170658668440543
5	0.6626126790368904	1.6668251309243094
6	0.41144462865833326	1.242003847004591
7	0.28360887270122315	0.9987991112425891
8	0.218212661615795	0.8782744062084924
9	0.15287703421503776	0.6922214487264656
>10	0.9579437195368954	9.305644532458231
>50	0.11785746211557716	4.195751924346366
>100	0.11587012763256581	12.565654902587195
>500	0.01422275900149057	4.816354088017954
>1k	0.005741297211610872	5.2345937856308105
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4184	0.27391915195049554	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	4162	0.2724788504823046	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3713	0.24308360688149855	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	3045	0.19935081684733721	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	2813	0.1841621831827782	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2697	0.17656786635049868	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	2545	0.16661669257027034	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	2513	0.16452170861653806	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	2141	0.14016752015440032	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	2005	0.13126383835103814	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	1954	0.12792495767477732	No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC	1872	0.12255656129333833	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	1858	0.12164000581358046	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	1850	0.1211162598251474	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1828	0.11967595835695646	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1819	0.11908674411996927	No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC	1772	0.11600973643792498	No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC	1700	0.11129602254202735	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	1700	0.11129602254202735	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	1685	0.11031399881371534	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	1685	0.11031399881371534	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	1611	0.10546934842070943	No Hit
GATGAACACCATTCTTGATAATCTTGCCGCCAGGGACTTTATA	1600	0.10474919768661396	No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA	1549	0.10141031701035315	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	6.546824855413373E-5	0.0
10	0.0	0.0	0.0	6.546824855413373E-5	0.0
11	0.0	0.0	0.0	1.3093649710826745E-4	0.0
12	0.0	0.0	0.0	1.3093649710826745E-4	0.0
13	0.0	0.0	0.0	1.3093649710826745E-4	0.0
14	0.0	0.0	0.0	2.618729942165349E-4	0.0
15	0.0	0.0	0.0	3.273412427706687E-4	0.0
16	0.0	0.0	0.0	8.510872312037385E-4	0.0
17	0.0	0.0	0.0	0.0014403014681909421	0.0
18	6.546824855413373E-5	0.0	0.0	0.0015057697167450757	0.0
19	6.546824855413373E-5	0.0	0.0	0.0018331109595157444	0.0
20	6.546824855413373E-5	0.0	0.0	0.0020949839537322793	0.0
21	6.546824855413373E-5	0.0	0.0	0.0028151346878277504	0.0
22	1.3093649710826745E-4	0.0	0.0	0.004255436156018692	0.0
23	1.3093649710826745E-4	0.0	0.0	0.006154015364088571	0.0
24	1.3093649710826745E-4	0.0	0.0	0.008707277057699787	0.0
25	1.3093649710826745E-4	0.0	0.0	0.010147578525890728	0.0
26	1.3093649710826745E-4	0.0	0.0	0.012242562479623008	0.0
27	1.3093649710826745E-4	0.0	0.0	0.022913886993946805	0.0
28	1.3093649710826745E-4	0.0	0.0	0.04595871048500188	0.0
29	1.3093649710826745E-4	0.0	0.0	0.07738346979098606	0.0
30	1.3093649710826745E-4	0.0	0.0	0.12124719632225567	0.0
31	1.3093649710826745E-4	0.0	0.0	0.24177424191041585	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTACGC	35	8.870738E-4	26.42857	33
GCTAGAC	70	1.21953075E-4	18.5	3
GCTTAGG	1075	0.0	18.241861	1
CTTAGGA	1150	0.0	17.856522	2
TAGGACA	1140	0.0	17.364035	4
TACGCTA	75	2.0678269E-4	17.266666	9
CGTCCTA	65	0.0015802557	17.076923	33
TTAGGAC	1190	0.0	16.945377	3
CCGAATT	90	4.447496E-5	16.444445	14
GTATTGG	160	6.2937033E-10	16.1875	1
ATACGCT	80	3.3834766E-4	16.1875	8
TATACGC	80	3.3834766E-4	16.1875	7
CGAATTA	95	7.062105E-5	15.578948	15
CTACAGT	155	7.212293E-9	15.5161295	4
CGTTATT	85	5.365586E-4	15.235294	2
GCCCGTA	195	4.1836756E-11	15.179486	18
TCGCCAT	670	0.0	14.634329	13
AGGACAC	1505	0.0	14.627906	5
CTAACAC	115	2.2106755E-5	14.47826	3
GGTATCA	2155	0.0	14.422275	1
>>END_MODULE