FastQCFastQC Report
Fri 10 Feb 2017
ERR1630159.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630159.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences452872
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA803.274181E-1127.751
CGCGTAA451.3214829E-424.66666610
TCGCGTA502.698599E-422.29
GCGTAAC502.698599E-422.211
CGTAGTA855.1632014E-821.7647062
TAACGAA450.00382228220.55555513
TATAGAC450.00382228220.5555552
CGTAACT555.136482E-420.18181812
CCGATAA500.007028459618.59
TAGTTAG609.2250365E-418.518
GTATATG500.007028459618.51
GTAACTA701.21712765E-418.513
TAATCTC500.007028459618.55
ACGAACG500.007028459618.515
TCTTATA2450.018.1224537
TAGACCA953.5966314E-617.5263164
GTCGCGT650.001577935617.0769238
GTTACGC650.001577935617.07692319
ATTAAAC1005.8619808E-616.6500023
GCTATAG803.3769067E-416.18753
Produced by FastQC (version 0.11.5)