##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630159.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	452872
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2277045169496	31.0	31.0	34.0	28.0	34.0
2	31.456179671077038	31.0	31.0	34.0	28.0	34.0
3	31.582902012047555	31.0	31.0	34.0	30.0	34.0
4	35.34104780158632	37.0	35.0	37.0	33.0	37.0
5	35.142181455245634	37.0	35.0	37.0	32.0	37.0
6	35.21584906993588	37.0	35.0	37.0	32.0	37.0
7	35.192663710717376	37.0	35.0	37.0	32.0	37.0
8	35.162571322581215	37.0	35.0	37.0	32.0	37.0
9	36.806342631030404	38.0	37.0	39.0	33.0	39.0
10	36.62370382801321	38.0	35.0	39.0	32.0	39.0
11	36.7398315638856	38.0	37.0	39.0	32.0	39.0
12	36.56880752177216	38.0	35.0	39.0	32.0	39.0
13	36.63721758024342	38.0	35.0	39.0	32.0	39.0
14	37.709003868642796	39.0	37.0	40.0	33.0	41.0
15	37.668716546838844	39.0	37.0	40.0	33.0	41.0
16	37.63093324383048	39.0	37.0	40.0	32.0	41.0
17	37.56792206186295	39.0	37.0	40.0	32.0	41.0
18	37.51112234803653	39.0	36.0	40.0	32.0	41.0
19	37.49136842198237	39.0	37.0	40.0	32.0	41.0
20	37.41104992139059	39.0	36.0	40.0	32.0	41.0
21	37.36752548181384	39.0	36.0	40.0	32.0	41.0
22	37.33598897701779	39.0	36.0	40.0	32.0	41.0
23	37.25256143016128	39.0	36.0	40.0	32.0	41.0
24	37.235686904909116	39.0	36.0	40.0	31.0	41.0
25	37.18376274090692	39.0	36.0	40.0	31.0	41.0
26	36.964137769612606	39.0	36.0	40.0	31.0	41.0
27	36.85099321662633	39.0	36.0	40.0	31.0	41.0
28	36.751868077514175	39.0	36.0	40.0	30.0	41.0
29	36.64833772015051	39.0	36.0	40.0	30.0	41.0
30	36.59112066985815	39.0	35.0	40.0	30.0	41.0
31	36.46459043614973	38.0	35.0	40.0	30.0	41.0
32	36.3546852090657	38.0	35.0	40.0	30.0	41.0
33	36.29329258598456	38.0	35.0	40.0	30.0	41.0
34	36.17299810984119	38.0	35.0	40.0	29.0	41.0
35	36.07249730608207	38.0	35.0	40.0	29.0	41.0
36	35.9678893815471	38.0	35.0	40.0	29.0	41.0
37	35.891673585472276	38.0	35.0	40.0	28.0	41.0
38	35.779617640304544	38.0	34.0	40.0	28.0	41.0
39	35.65451606634987	38.0	34.0	40.0	27.0	41.0
40	35.561074652440425	38.0	34.0	40.0	27.0	41.0
41	35.41740712607536	38.0	34.0	40.0	27.0	41.0
42	35.353868201169426	38.0	34.0	40.0	27.0	41.0
43	34.02928421275769	37.0	32.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	2.0
14	2.0
15	4.0
16	7.0
17	19.0
18	33.0
19	75.0
20	163.0
21	311.0
22	562.0
23	959.0
24	1554.0
25	2299.0
26	3283.0
27	4663.0
28	6343.0
29	8400.0
30	11102.0
31	14049.0
32	17714.0
33	22522.0
34	29057.0
35	38434.0
36	52703.0
37	77019.0
38	109701.0
39	51889.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.782887880019075	18.977326926813756	12.375240686109983	26.864544507057182
2	20.741401543924113	20.17921178611175	31.85403381087813	27.225352859086012
3	21.09646875938455	21.791146284159762	28.168444946916566	28.94394000953912
4	16.535356568743488	15.352903248600047	31.85624194032751	36.25549824232896
5	16.328675652281436	34.92598350085675	33.17007896270911	15.575261884152697
6	37.9910879895423	32.96869755692558	14.153005705806498	14.887208747725628
7	31.270204384461834	28.840599551308095	19.880231058665583	20.008965005564487
8	28.669027893091204	32.53126711300323	18.322616545072336	20.477088448833225
9	27.277464714091398	13.471797770672508	17.8962267483969	41.3545107668392
10	17.964457948382766	25.59906551961702	31.94942500309138	24.487051528908832
11	39.063134837216694	20.299775654047945	19.258863431609814	21.378226077125547
12	22.88770336872229	23.747548976311187	27.580861700436326	25.7838859545302
13	32.247080852867924	18.376274090692295	22.632001978483988	26.744643077955804
14	23.92927802999523	19.01508594039817	22.79959900369199	34.25603702591461
15	27.043182179512094	26.187752830821953	20.014485329187938	26.75457966047802
16	27.13989824939497	24.631904820788215	21.82228090939603	26.405916020420783
17	25.86426186648766	24.756664134678232	23.044922185518203	26.334151813315902
18	26.1775954353548	23.751965235209948	23.9557755833878	26.11466374604745
19	26.704455121977073	23.89483121058489	23.452322068928968	25.94839159850907
20	26.75325478280839	23.53843911745482	23.43244890388454	26.275857195852247
21	27.054443639703933	23.67026444558286	23.069874048296207	26.205417866417
22	27.057755833878005	23.567807239131586	23.109399565440125	26.26503736155028
23	26.587865887049762	23.617710964687593	23.405730537547033	26.38869261071561
24	26.688335776996592	23.963062410570757	22.82079704640605	26.527804766026602
25	26.984445936158565	23.452322068928968	23.248732533696057	26.31449946121641
26	26.661617410659083	23.99066402868802	23.18624247027858	26.161476090374325
27	26.770036566623684	23.460492147891678	23.194854175131162	26.574617110353476
28	26.683698705152892	23.581497641717746	23.42184988252751	26.312953770601847
29	26.374339769294636	23.7186224805243	23.666952251408787	26.24008549877228
30	26.24913882951474	24.109903018954583	23.30592308643502	26.335035065095656
31	26.836722075994984	23.876503736155026	23.17255206769242	26.11422212015757
32	26.192389902665653	23.74975710576057	23.273021957639244	26.78483103393453
33	26.083087494921305	23.708244272112207	23.54793407408716	26.66073415887933
34	26.76562030772492	23.146496140189722	23.538218304509883	26.549665247575472
35	26.350271158296383	23.61152820222933	23.975869561377166	26.062331078097124
36	26.379197654083274	23.666952251408787	23.51547457118126	26.43837552332668
37	25.972018583617444	23.15245808970305	24.31084279884824	26.564680527831264
38	25.832243989471635	23.25624017382395	24.498312989100672	26.41320284760374
39	25.786535709869458	22.871804836686746	24.85404264339593	26.48761681004787
40	25.667296719602888	22.869817520182302	25.131825328128038	26.331060432086772
41	25.17819604656503	22.730484551926374	25.62026356233108	26.471055839177517
42	25.127188256284338	22.836916391386527	25.598623893727147	26.43727145860199
43	24.940601317811655	22.23542192937519	25.91747778621774	26.906498966595414
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	6.5
2	12.0
3	15.0
4	18.0
5	18.0
6	32.5
7	47.0
8	42.5
9	38.0
10	56.5
11	75.0
12	75.0
13	149.0
14	223.0
15	312.5
16	402.0
17	435.0
18	468.0
19	468.0
20	649.0
21	830.0
22	934.0
23	1038.0
24	1401.0
25	1764.0
26	1764.0
27	2260.5
28	2757.0
29	3649.5
30	4542.0
31	5555.0
32	6568.0
33	6568.0
34	8047.0
35	9526.0
36	11525.5
37	13525.0
38	15836.5
39	18148.0
40	18148.0
41	20697.5
42	23247.0
43	25384.0
44	27521.0
45	29599.0
46	31677.0
47	31677.0
48	32980.5
49	34284.0
50	35462.5
51	36641.0
52	37535.0
53	38429.0
54	38429.0
55	37644.5
56	36860.0
57	35937.0
58	35014.0
59	33746.0
60	32478.0
61	32478.0
62	30324.0
63	28170.0
64	25047.0
65	21924.0
66	19229.5
67	16535.0
68	16535.0
69	14017.0
70	11499.0
71	9762.0
72	8025.0
73	6292.5
74	4560.0
75	4560.0
76	3618.0
77	2676.0
78	2122.0
79	1568.0
80	1257.5
81	947.0
82	947.0
83	696.5
84	446.0
85	327.5
86	209.0
87	155.5
88	102.0
89	102.0
90	78.5
91	55.0
92	35.0
93	15.0
94	9.5
95	4.0
96	4.0
97	3.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	452872.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.31731642109958
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.27162153246653	62.3893215252473
2	8.1780342058404	11.82826974732672
3	2.301914629524941	4.994048659131401
4	1.02554512561858	2.966586854139006
5	0.5768579781083014	2.0858410466446884
6	0.353336211509847	1.5331395966474104
7	0.2329771005695727	1.1793795090532089
8	0.18393502754668184	1.0641350070413647
9	0.1357282401623998	0.8833951881992935
>10	0.7052307794264933	8.973107887860673
>50	0.028405111860506586	1.4251159918090235
>100	0.006414057365801909	0.6776589868999116
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	2.2081294493808405E-4	0.0
13	0.0	0.0	0.0	2.2081294493808405E-4	0.0
14	0.0	0.0	0.0	2.2081294493808405E-4	0.0
15	0.0	0.0	0.0	2.2081294493808405E-4	0.0
16	0.0	0.0	0.0	2.2081294493808405E-4	0.0
17	0.0	0.0	0.0	2.2081294493808405E-4	0.0
18	0.0	0.0	0.0	2.2081294493808405E-4	0.0
19	0.0	0.0	0.0	4.416258898761681E-4	0.0
20	0.0	0.0	0.0	4.416258898761681E-4	0.0
21	0.0	0.0	0.0	6.624388348142521E-4	0.0
22	0.0	0.0	0.0	0.0013248776696285043	0.0
23	0.0	0.0	0.0	0.0017665035595046724	0.0
24	0.0	0.0	0.0	0.002870568284195093	0.0
25	0.0	0.0	0.0	0.0030913812291331767	0.0
26	0.0	0.0	0.0	0.004195445953823597	0.0
27	0.0	0.0	0.0	0.01081983430196612	0.0
28	0.0	2.2081294493808405E-4	0.0	0.028926495786889012	0.0
29	0.0	2.2081294493808405E-4	0.0	0.05939868218834461	0.0
30	0.0	2.2081294493808405E-4	0.0	0.0995866381670759	0.0
31	0.0	2.2081294493808405E-4	0.0	0.23052871451535975	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATAGA	80	3.274181E-11	27.75	1
CGCGTAA	45	1.3214829E-4	24.666666	10
TCGCGTA	50	2.698599E-4	22.2	9
GCGTAAC	50	2.698599E-4	22.2	11
CGTAGTA	85	5.1632014E-8	21.764706	2
TAACGAA	45	0.003822282	20.555555	13
TATAGAC	45	0.003822282	20.555555	2
CGTAACT	55	5.136482E-4	20.181818	12
CCGATAA	50	0.0070284596	18.5	9
TAGTTAG	60	9.2250365E-4	18.5	18
GTATATG	50	0.0070284596	18.5	1
GTAACTA	70	1.21712765E-4	18.5	13
TAATCTC	50	0.0070284596	18.5	5
ACGAACG	50	0.0070284596	18.5	15
TCTTATA	245	0.0	18.12245	37
TAGACCA	95	3.5966314E-6	17.526316	4
GTCGCGT	65	0.0015779356	17.076923	8
GTTACGC	65	0.0015779356	17.076923	19
ATTAAAC	100	5.8619808E-6	16.650002	3
GCTATAG	80	3.3769067E-4	16.1875	3
>>END_MODULE