FastQCFastQC Report
Fri 10 Feb 2017
ERR1630158.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630158.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences372575
Sequences flagged as poor quality0
Sequence length43
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT168434.5207005300946115No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT122483.287391800308663No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT101312.719184056901295No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52631.4126014896329597No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA24340.6532912836341677No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA17870.47963497282426354No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA16860.4525263369791317No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15970.4286385291552036No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13710.36797960142253233No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11400.3059786620143595No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11380.3054418573441589No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA9870.2649131047440113No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA8180.219553110112058No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA7900.21203784472924914No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA7830.21015902838354694No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7500.20130175132523653No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA6950.18653962289471918No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6850.18385559954371605No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA6840.18358719720861572No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA6450.17311950613970342No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA6370.1709722874589009No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5790.1554049520230826No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5610.15057370999127692No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA5120.13742199557136148No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA4940.1325907535395558No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG4870.13071193719385357No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA4850.13017513252365295No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA4680.1256122928269476No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4630.12427028115144603No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA4610.12373347648124539No Hit
ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA4530.12158625780044287No Hit
GTACATGGGAAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA4330.11621821109843655No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4260.11433939475273434No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA4220.11326578541233309No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA4080.10950815272092868No Hit
GAACATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA4060.10897134805072804No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC3940.10575052002952427No Hit
TATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAA3940.10575052002952427No Hit
GTACATGGGAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAA3920.10521371535932363No Hit
GTACATGGGAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA3890.10440850835402267No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT3770.1011876803328189No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAATAC200.0018403437.01
CAATTAG451.07664164E-732.88888517
CGTCATT551.848639E-830.27272826
TTAGGTC502.7124406E-729.59999820
ATTAGGT502.7124406E-729.59999819
TAGGTCG502.7124406E-729.59999821
TAAGTTC250.005491635829.5999983
AAGTTCC250.005491635829.5999984
CTGTGCG604.3062755E-827.7500029
GCCGGAA405.929522E-527.7515
ACAGAGG405.929522E-527.753
TAGTACT556.2426625E-726.909094
GGTCGTC601.3333556E-624.66666823
CATTGCG601.3333556E-624.66666829
GTCGTCA601.3333556E-624.66666824
ACTGTGC601.3333556E-624.6666688
GCCAATT601.3333556E-624.66666815
CGCCAAT601.3333556E-624.66666814
CTAGTAC751.3673343E-824.6666663
TTAGGAC451.3208686E-424.6666643
Produced by FastQC (version 0.11.5)