FastQCFastQC Report
Fri 10 Feb 2017
ERR1630157.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630157.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2238910
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC121690.5435234109455047No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT68760.30711372944870496No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT44590.19915941239263749No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG43160.19277237584360246No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG42200.18848457508341113No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG40380.18035561947554837No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT40170.17941766305925652No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT38240.17079739694762183No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG37840.1690108132975421No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT36160.16150716196720727No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG34100.15230625616929666No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG32770.14636586553278158No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC31870.1423460523201022No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG31180.13926419552371466No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG29860.13336846947845157No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT29720.13274316520092366No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT29620.13229651928840372No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC29120.13006328972580408No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG28040.1252395138705888No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT26020.1162172664376862No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT25280.1129120866850387No Hit
TGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGA24690.1102768758011711No Hit
GGTCCCCAGAGGGCCAGCAGCGCCAGCAGGGGCAGGAGGCGCA24100.10764166491730352No Hit
GCTCACACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACG24010.10723968359603556No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGGC28650.020.7277497
TGCATCA28050.020.5775414
CAGGACA31550.020.112524
GGACAGG31750.020.0440946
ACAGGCT29750.019.774798
GGTATCA14350.019.595821
CCAGGAC31550.019.5261483
GCTGCAT29900.019.48996512
GCATCAG29800.019.43120815
CTGCATC30350.019.01812213
CGCACTA500.00703691418.527
TCCAGGA33700.018.335312
ATCAAGC31500.018.32380930
AGAGGCC32450.018.12942923
AGGCCAT32000.018.09531425
GGCCATC32550.018.01689726
GTCTATA852.7249827E-517.4117641
CATCAAG33450.017.36621929
CAAGCAG32150.017.26282932
CCATCAA33600.017.23363328
Produced by FastQC (version 0.11.5)