FastQCFastQC Report
Fri 10 Feb 2017
ERR1630156.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630156.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences833657
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18340.21999455411518165No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17320.20775930628543876No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12930.15509975925350594No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC9570.11479541346141159No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA9280.11131676456864154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGA759.260764E-619.7333341
TCGCCTA609.2339126E-418.535
TAACCCA801.6158021E-518.55
AGCCTTA609.2339126E-418.56
TCTAGCG1351.1496013E-917.81481628
CTAGCGG1351.1496013E-917.81481629
GTCCTAA852.7216633E-517.4117641
TACCCTT650.001579442217.0769235
CGGTCCA1751.3096724E-1015.85714310
GTCCTAT1302.5862755E-715.6538461
GAATAGA855.361957E-415.2352931
GTATAGT1353.9680344E-715.0740751
GGTATCA8600.015.0581391
AAGACGG1601.0946678E-815.0312515
TAGAACA750.004103148414.84
GCGTATA750.004103148414.89
CCGGTCC2006.184564E-1114.7999999
CGACGGT1152.2082948E-514.478267
TACGACG1152.2082948E-514.478265
CTAGGAC908.270653E-414.3888883
Produced by FastQC (version 0.11.5)