FastQCFastQC Report
Fri 10 Feb 2017
ERR1630155.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630155.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89819
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9881.0999899798483617No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT8670.9652746078223983No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5810.6468564557610306No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3130.3484786069762522No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA2060.2293501374987475No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1920.21376323495028893No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA1560.17368262839710974No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA1550.172569278215077No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1550.172569278215077No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT1500.16700252730491322No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC1340.14918892439238915No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA1310.14584887384629086No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG1300.14473552366425813No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1260.1402821229361271No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG1230.13694207239002884No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC1200.13360202184393058No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC1150.1280352709337668No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC1130.1258085705697013No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA1120.12469522038766852No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC1120.12469522038766852No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1110.12358187020563578No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1070.11912846947750476No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC1000.11133501820327547No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG970.10799496765717721No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCG950.1057682672931117No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG930.10354156692904619No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCT920.10242821674701344No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA910.10131486656498069No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC900.10020151638294793No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCAGAT303.5733476E-430.83333416
CAGATAA303.5733476E-430.83333418
TCAGATA303.5733476E-430.83333417
CTATGTA250.005469735229.69
AGATAAC250.005469735229.619
CGTTACC250.005469735229.62
ACGATGA250.005469735229.624
ACAGGGC250.005469735229.634
CCTATGT250.005469735229.68
GACCTTA250.005469735229.629
AACAGGG250.005469735229.633
TCAACAA250.005469735229.618
ATAGAGC358.805615E-426.42857237
TATGTAT358.805615E-426.42857210
ATGTATT400.001917431623.12500211
GAGGCCA400.001917431623.1250029
ACTAACA603.6772908E-521.58333433
CCACTAA705.0160634E-621.14285931
TCGCGCG705.0160634E-621.1428599
CCTTATT450.00379881720.55555727
Produced by FastQC (version 0.11.5)